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Article: An ultrastructural study of the application of dentine adhesives to acid-conditioned sclerotic dentine
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TitleAn ultrastructural study of the application of dentine adhesives to acid-conditioned sclerotic dentine
 
AuthorsKwong, SM1
Tay, FR1
Yip, HK1
Kei, LH1
Pashley, DH2
 
KeywordsAcid-conditioned sclerotic dentine
Dentine adhesives
Ultrastructural study
 
Issue Date2000
 
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
 
CitationJournal Of Dentistry, 2000, v. 28 n. 7, p. 515-528 [How to Cite?]
 
AbstractObjective: This in vitro study examined the ultrastructure of resin-infiltrated sclerotic dentine following the application of a self-etching primer, with or without the adjunctive use of phosphoric acid pre-conditioning. Materials and Methods: Non-carious, natural cervical sclerotic lesions were hand-cleaned with a slurry of pumice and chlorhexidine and bonded without further cavity preparation. One group was bonded using Clearfil Liner Bond 2V (Kuraray Co. Ltd, Osaka, Japan) alone. Specimens from the other group were pre-conditioned with K-etchant (40% phosphoric acid gel, Kuraray) prior to the application of the same self-etching primer. Artificially prepared wedge-shaped lesions were also made in sound bicuspids, bonded using the two methods, and used as controls for the two groups. For SEM examination, each specimen was cryofractured into two halves through a pre-formed slit made on the lingual surface, after the respective conditioning treatment. Different locations within the lesions were examined after rinsing of the phosphoric acid/self-etching primer and specimen dehydration. For TEM investigation, specimens were bonded with the adhesive. Both demineralised and undemineralised ultrathin sections were prepared from the occlusal, gingival and deepest part of the wedge-shaped bonded lesions following specimen fixation, dehydration and resin embedding. Results: A hypermineralised surface layer was present on the surface of etched sclerotic dentine. This layer was thicker in the deepest part of the natural lesions, where bacterial colonisation of the lesion surface was also apparent. Both treatment protocols were unable to effectively dissolve sclerotic casts that occluded the dentinal tubules. Depending upon the thickness of the surface layers at different locations in the natural lesion, self-etching primer treatment alone resulted in reduction of the thickness of the authentic hybrid layer (i.e. hybridised intertubular dentine). This was also true of phosphoric acid pre-conditioning along the deepest part of the natural lesions. Within this region, intertubular dentine completely devoid of an authentic hybrid layer could be seen in both treatment groups. Resin tags were also sparsely observed in such regions. Conclusions: Adhesive strategies that rely mostly on micromechanical retention alone may be compromised by the sporadic absence of the hybrid layer and resin tags in sclerotic dentine. Based on the ultrastructural features presented, it is further speculated that adaptive strategies such as removal of the surface layers and extended etching time may not be completely effective in improving bonding efficacy in highly sclerotic dentine. Interdisciplinary research should be continued to develop alternative procedures for bonding resins equally well to sound and sclerotic dentine. © 2000 Elsevier Science Ltd. All rights reserved.
 
ISSN0300-5712
2013 Impact Factor: 2.840
2013 SCImago Journal Rankings: 1.159
 
ISI Accession Number IDWOS:000089146800009
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorKwong, SM
 
dc.contributor.authorTay, FR
 
dc.contributor.authorYip, HK
 
dc.contributor.authorKei, LH
 
dc.contributor.authorPashley, DH
 
dc.date.accessioned2010-09-06T05:42:19Z
 
dc.date.available2010-09-06T05:42:19Z
 
dc.date.issued2000
 
dc.description.abstractObjective: This in vitro study examined the ultrastructure of resin-infiltrated sclerotic dentine following the application of a self-etching primer, with or without the adjunctive use of phosphoric acid pre-conditioning. Materials and Methods: Non-carious, natural cervical sclerotic lesions were hand-cleaned with a slurry of pumice and chlorhexidine and bonded without further cavity preparation. One group was bonded using Clearfil Liner Bond 2V (Kuraray Co. Ltd, Osaka, Japan) alone. Specimens from the other group were pre-conditioned with K-etchant (40% phosphoric acid gel, Kuraray) prior to the application of the same self-etching primer. Artificially prepared wedge-shaped lesions were also made in sound bicuspids, bonded using the two methods, and used as controls for the two groups. For SEM examination, each specimen was cryofractured into two halves through a pre-formed slit made on the lingual surface, after the respective conditioning treatment. Different locations within the lesions were examined after rinsing of the phosphoric acid/self-etching primer and specimen dehydration. For TEM investigation, specimens were bonded with the adhesive. Both demineralised and undemineralised ultrathin sections were prepared from the occlusal, gingival and deepest part of the wedge-shaped bonded lesions following specimen fixation, dehydration and resin embedding. Results: A hypermineralised surface layer was present on the surface of etched sclerotic dentine. This layer was thicker in the deepest part of the natural lesions, where bacterial colonisation of the lesion surface was also apparent. Both treatment protocols were unable to effectively dissolve sclerotic casts that occluded the dentinal tubules. Depending upon the thickness of the surface layers at different locations in the natural lesion, self-etching primer treatment alone resulted in reduction of the thickness of the authentic hybrid layer (i.e. hybridised intertubular dentine). This was also true of phosphoric acid pre-conditioning along the deepest part of the natural lesions. Within this region, intertubular dentine completely devoid of an authentic hybrid layer could be seen in both treatment groups. Resin tags were also sparsely observed in such regions. Conclusions: Adhesive strategies that rely mostly on micromechanical retention alone may be compromised by the sporadic absence of the hybrid layer and resin tags in sclerotic dentine. Based on the ultrastructural features presented, it is further speculated that adaptive strategies such as removal of the surface layers and extended etching time may not be completely effective in improving bonding efficacy in highly sclerotic dentine. Interdisciplinary research should be continued to develop alternative procedures for bonding resins equally well to sound and sclerotic dentine. © 2000 Elsevier Science Ltd. All rights reserved.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationJournal Of Dentistry, 2000, v. 28 n. 7, p. 515-528 [How to Cite?]
 
dc.identifier.epage528
 
dc.identifier.hkuros58029
 
dc.identifier.isiWOS:000089146800009
 
dc.identifier.issn0300-5712
2013 Impact Factor: 2.840
2013 SCImago Journal Rankings: 1.159
 
dc.identifier.issue7
 
dc.identifier.openurl
 
dc.identifier.pmid10960756
 
dc.identifier.scopuseid_2-s2.0-0034264414
 
dc.identifier.spage515
 
dc.identifier.urihttp://hdl.handle.net/10722/65947
 
dc.identifier.volume28
 
dc.languageeng
 
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofJournal of Dentistry
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Dentistry. Copyright © Elsevier Ltd.
 
dc.subjectAcid-conditioned sclerotic dentine
 
dc.subjectDentine adhesives
 
dc.subjectUltrastructural study
 
dc.titleAn ultrastructural study of the application of dentine adhesives to acid-conditioned sclerotic dentine
 
dc.typeArticle
 
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Author Affiliations
  1. Prince Philip Dental Hospital
  2. Medical College of Georgia