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Article: An ultrastructural study of the application of dentine adhesives to acid-conditioned sclerotic dentine

TitleAn ultrastructural study of the application of dentine adhesives to acid-conditioned sclerotic dentine
Authors
KeywordsAcid-conditioned sclerotic dentine
Dentine adhesives
Ultrastructural study
Issue Date2000
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdent
Citation
Journal Of Dentistry, 2000, v. 28 n. 7, p. 515-528 How to Cite?
Abstract
Objective: This in vitro study examined the ultrastructure of resin-infiltrated sclerotic dentine following the application of a self-etching primer, with or without the adjunctive use of phosphoric acid pre-conditioning. Materials and Methods: Non-carious, natural cervical sclerotic lesions were hand-cleaned with a slurry of pumice and chlorhexidine and bonded without further cavity preparation. One group was bonded using Clearfil Liner Bond 2V (Kuraray Co. Ltd, Osaka, Japan) alone. Specimens from the other group were pre-conditioned with K-etchant (40% phosphoric acid gel, Kuraray) prior to the application of the same self-etching primer. Artificially prepared wedge-shaped lesions were also made in sound bicuspids, bonded using the two methods, and used as controls for the two groups. For SEM examination, each specimen was cryofractured into two halves through a pre-formed slit made on the lingual surface, after the respective conditioning treatment. Different locations within the lesions were examined after rinsing of the phosphoric acid/self-etching primer and specimen dehydration. For TEM investigation, specimens were bonded with the adhesive. Both demineralised and undemineralised ultrathin sections were prepared from the occlusal, gingival and deepest part of the wedge-shaped bonded lesions following specimen fixation, dehydration and resin embedding. Results: A hypermineralised surface layer was present on the surface of etched sclerotic dentine. This layer was thicker in the deepest part of the natural lesions, where bacterial colonisation of the lesion surface was also apparent. Both treatment protocols were unable to effectively dissolve sclerotic casts that occluded the dentinal tubules. Depending upon the thickness of the surface layers at different locations in the natural lesion, self-etching primer treatment alone resulted in reduction of the thickness of the authentic hybrid layer (i.e. hybridised intertubular dentine). This was also true of phosphoric acid pre-conditioning along the deepest part of the natural lesions. Within this region, intertubular dentine completely devoid of an authentic hybrid layer could be seen in both treatment groups. Resin tags were also sparsely observed in such regions. Conclusions: Adhesive strategies that rely mostly on micromechanical retention alone may be compromised by the sporadic absence of the hybrid layer and resin tags in sclerotic dentine. Based on the ultrastructural features presented, it is further speculated that adaptive strategies such as removal of the surface layers and extended etching time may not be completely effective in improving bonding efficacy in highly sclerotic dentine. Interdisciplinary research should be continued to develop alternative procedures for bonding resins equally well to sound and sclerotic dentine. © 2000 Elsevier Science Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/65947
ISSN
2013 Impact Factor: 2.840
2013 SCImago Journal Rankings: 1.159
ISI Accession Number ID
References

 

Author Affiliations
  1. Prince Philip Dental Hospital
  2. Medical College of Georgia
DC FieldValueLanguage
dc.contributor.authorKwong, SMen_HK
dc.contributor.authorTay, FRen_HK
dc.contributor.authorYip, HKen_HK
dc.contributor.authorKei, LHen_HK
dc.contributor.authorPashley, DHen_HK
dc.date.accessioned2010-09-06T05:42:19Z-
dc.date.available2010-09-06T05:42:19Z-
dc.date.issued2000en_HK
dc.identifier.citationJournal Of Dentistry, 2000, v. 28 n. 7, p. 515-528en_HK
dc.identifier.issn0300-5712en_HK
dc.identifier.urihttp://hdl.handle.net/10722/65947-
dc.description.abstractObjective: This in vitro study examined the ultrastructure of resin-infiltrated sclerotic dentine following the application of a self-etching primer, with or without the adjunctive use of phosphoric acid pre-conditioning. Materials and Methods: Non-carious, natural cervical sclerotic lesions were hand-cleaned with a slurry of pumice and chlorhexidine and bonded without further cavity preparation. One group was bonded using Clearfil Liner Bond 2V (Kuraray Co. Ltd, Osaka, Japan) alone. Specimens from the other group were pre-conditioned with K-etchant (40% phosphoric acid gel, Kuraray) prior to the application of the same self-etching primer. Artificially prepared wedge-shaped lesions were also made in sound bicuspids, bonded using the two methods, and used as controls for the two groups. For SEM examination, each specimen was cryofractured into two halves through a pre-formed slit made on the lingual surface, after the respective conditioning treatment. Different locations within the lesions were examined after rinsing of the phosphoric acid/self-etching primer and specimen dehydration. For TEM investigation, specimens were bonded with the adhesive. Both demineralised and undemineralised ultrathin sections were prepared from the occlusal, gingival and deepest part of the wedge-shaped bonded lesions following specimen fixation, dehydration and resin embedding. Results: A hypermineralised surface layer was present on the surface of etched sclerotic dentine. This layer was thicker in the deepest part of the natural lesions, where bacterial colonisation of the lesion surface was also apparent. Both treatment protocols were unable to effectively dissolve sclerotic casts that occluded the dentinal tubules. Depending upon the thickness of the surface layers at different locations in the natural lesion, self-etching primer treatment alone resulted in reduction of the thickness of the authentic hybrid layer (i.e. hybridised intertubular dentine). This was also true of phosphoric acid pre-conditioning along the deepest part of the natural lesions. Within this region, intertubular dentine completely devoid of an authentic hybrid layer could be seen in both treatment groups. Resin tags were also sparsely observed in such regions. Conclusions: Adhesive strategies that rely mostly on micromechanical retention alone may be compromised by the sporadic absence of the hybrid layer and resin tags in sclerotic dentine. Based on the ultrastructural features presented, it is further speculated that adaptive strategies such as removal of the surface layers and extended etching time may not be completely effective in improving bonding efficacy in highly sclerotic dentine. Interdisciplinary research should be continued to develop alternative procedures for bonding resins equally well to sound and sclerotic dentine. © 2000 Elsevier Science Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/jdenten_HK
dc.relation.ispartofJournal of Dentistryen_HK
dc.rightsJournal of Dentistry. Copyright © Elsevier Ltd.en_HK
dc.subjectAcid-conditioned sclerotic dentineen_HK
dc.subjectDentine adhesivesen_HK
dc.subjectUltrastructural studyen_HK
dc.titleAn ultrastructural study of the application of dentine adhesives to acid-conditioned sclerotic dentineen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0300-5712&volume=28&spage=515&epage=528&date=2000&atitle=An+ultrastructural+study+of+the+application+of+dentine+adhesives+to+acid-conditioned+sclerotic+dentineen_HK
dc.identifier.emailYip, HK: kevin.h.k.yip@hkusua.hku.hken_HK
dc.identifier.authorityYip, HK=rp00027en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid10960756en_HK
dc.identifier.scopuseid_2-s2.0-0034264414en_HK
dc.identifier.hkuros58029en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0034264414&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume28en_HK
dc.identifier.issue7en_HK
dc.identifier.spage515en_HK
dc.identifier.epage528en_HK
dc.identifier.isiWOS:000089146800009-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridKwong, SM=7005600678en_HK
dc.identifier.scopusauthoridTay, FR=7102091962en_HK
dc.identifier.scopusauthoridYip, HK=25423244900en_HK
dc.identifier.scopusauthoridKei, LH=6602484458en_HK
dc.identifier.scopusauthoridPashley, DH=35448600800en_HK

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