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Article: The biological behaviour of human adenoid cystic carcinoma cells transduced with interleukin-2-gene

TitleThe biological behaviour of human adenoid cystic carcinoma cells transduced with interleukin-2-gene
Authors
Issue Date2002
PublisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijom
Citation
International Journal Of Oral And Maxillofacial Surgery, 2002, v. 31 n. 6, p. 650-656 How to Cite?
AbstractAdenoid cystic carcinoma (ACC) of the salivary glands is a highly infiltrative malignant tumour with a tendency for lung metastasis. Gene therapy could be a potentially effective therapy for ACC and its metastasis. The aims of the study were: To transduce interleukin-2 (IL-2) gene into an ACC cell line with predisposition for lung metastasis (ACC-M); to compare the bioactivity of the gene-transduced cells and the parent cell line in vitro and in vivo. The IL-2 gene was transduced via a bicistronic retroviral vector into the ACC-M cells. The growth rate and DNA cell cycles of the parent ACC-M, the control viral vector AmGCEN, and the gene transduced AmIL-2 cell cultures were compared quantitatively and by flow cytometry, respectively. The tumourigenic ability of the three cell lines was verified by inoculation in athymic nude mice. The tumours developed were extracted and compared quantitatively and histologically. There was no difference in the growth rate and the DNA count between the ACC-M, AmGCEN, and AmIL-2 cell cultures. In the animal experiment, both the ACC-M and AmGCEN cells stimulated lung metastasis in all the mice, whereas there was no tumour found in the 1 × 10 6 AmIL-2 cells inoculation. On 3 × 10 6 AmIL-2 cells stimulation, three out of six mice developed tumours but the mass and volume of the tumours were smaller than the other two groups. Under light microscopy, the ACC-M tumours were mainly poorly differentiated with minimal cellular matrix, whereas the AmIL-2 tumours were well differentiated with ample matrix. The transduction of IL-2 gene can reduce the tumourigenicity of ACC-M cells and induces tumour cell differentiation in mice. The IL-2 gene can be a potential effective gene for the treatment of adenoid cystic carcinoma of salivary glands and its lung metastasis.
Persistent Identifierhttp://hdl.handle.net/10722/65945
ISSN
2015 Impact Factor: 1.563
2015 SCImago Journal Rankings: 0.854
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSun, CXen_HK
dc.contributor.authorHe, RGen_HK
dc.contributor.authorCheung, LKen_HK
dc.contributor.authorZhang, ZYen_HK
dc.contributor.authorChen, WTen_HK
dc.contributor.authorLiu, XKen_HK
dc.contributor.authorZhou, XJen_HK
dc.contributor.authorTang, ZYen_HK
dc.contributor.authorChen, SSen_HK
dc.date.accessioned2010-09-06T05:42:18Z-
dc.date.available2010-09-06T05:42:18Z-
dc.date.issued2002en_HK
dc.identifier.citationInternational Journal Of Oral And Maxillofacial Surgery, 2002, v. 31 n. 6, p. 650-656en_HK
dc.identifier.issn0901-5027en_HK
dc.identifier.urihttp://hdl.handle.net/10722/65945-
dc.description.abstractAdenoid cystic carcinoma (ACC) of the salivary glands is a highly infiltrative malignant tumour with a tendency for lung metastasis. Gene therapy could be a potentially effective therapy for ACC and its metastasis. The aims of the study were: To transduce interleukin-2 (IL-2) gene into an ACC cell line with predisposition for lung metastasis (ACC-M); to compare the bioactivity of the gene-transduced cells and the parent cell line in vitro and in vivo. The IL-2 gene was transduced via a bicistronic retroviral vector into the ACC-M cells. The growth rate and DNA cell cycles of the parent ACC-M, the control viral vector AmGCEN, and the gene transduced AmIL-2 cell cultures were compared quantitatively and by flow cytometry, respectively. The tumourigenic ability of the three cell lines was verified by inoculation in athymic nude mice. The tumours developed were extracted and compared quantitatively and histologically. There was no difference in the growth rate and the DNA count between the ACC-M, AmGCEN, and AmIL-2 cell cultures. In the animal experiment, both the ACC-M and AmGCEN cells stimulated lung metastasis in all the mice, whereas there was no tumour found in the 1 × 10 6 AmIL-2 cells inoculation. On 3 × 10 6 AmIL-2 cells stimulation, three out of six mice developed tumours but the mass and volume of the tumours were smaller than the other two groups. Under light microscopy, the ACC-M tumours were mainly poorly differentiated with minimal cellular matrix, whereas the AmIL-2 tumours were well differentiated with ample matrix. The transduction of IL-2 gene can reduce the tumourigenicity of ACC-M cells and induces tumour cell differentiation in mice. The IL-2 gene can be a potential effective gene for the treatment of adenoid cystic carcinoma of salivary glands and its lung metastasis.en_HK
dc.languageengen_HK
dc.publisherChurchill Livingstone. The Journal's web site is located at http://www.elsevier.com/locate/ijomen_HK
dc.relation.ispartofInternational Journal of Oral and Maxillofacial Surgeryen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCarcinoma, Adenoid Cystic - genetics - pathology - secondaryen_HK
dc.subject.meshCell Cycle - geneticsen_HK
dc.subject.meshCell Differentiation - geneticsen_HK
dc.subject.meshCell Division - geneticsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshDNA, Neoplasm - geneticsen_HK
dc.subject.meshDisease Models, Animalen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshFlow Cytometryen_HK
dc.subject.meshGenes - geneticsen_HK
dc.subject.meshGenetic Vectors - geneticsen_HK
dc.subject.meshHistocompatibility Antigens Class I - analysisen_HK
dc.subject.meshHistocompatibility Antigens Class II - analysisen_HK
dc.subject.meshHumansen_HK
dc.subject.meshInterleukin-2 - genetics - secretionen_HK
dc.subject.meshLung Neoplasms - genetics - secondaryen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Nudeen_HK
dc.subject.meshRetroviridae - geneticsen_HK
dc.subject.meshSalivary Gland Neoplasms - geneticsen_HK
dc.subject.meshTransduction, Genetic - methodsen_HK
dc.subject.meshTumor Cells, Cultureden_HK
dc.titleThe biological behaviour of human adenoid cystic carcinoma cells transduced with interleukin-2-geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0901-5027&volume=31&issue=6&spage=650&epage=6&date=2002&atitle=The+biological+behaviour+of+human+adenoid+cystic+carcinoma+cells+transduced+with+interleukin-2-geneen_HK
dc.identifier.emailCheung, LK:lkcheung@hkucc.hku.hken_HK
dc.identifier.authorityCheung, LK=rp00013en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1054/ijom.2002.0318en_HK
dc.identifier.pmid12521324-
dc.identifier.scopuseid_2-s2.0-0036906602en_HK
dc.identifier.hkuros79083en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036906602&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue6en_HK
dc.identifier.spage650en_HK
dc.identifier.epage656en_HK
dc.identifier.isiWOS:000179904300014-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridSun, CX=55238379200en_HK
dc.identifier.scopusauthoridHe, RG=7202418252en_HK
dc.identifier.scopusauthoridCheung, LK=7102302747en_HK
dc.identifier.scopusauthoridZhang, ZY=15039832100en_HK
dc.identifier.scopusauthoridChen, WT=7409642454en_HK
dc.identifier.scopusauthoridLiu, XK=37045886800en_HK
dc.identifier.scopusauthoridZhou, XJ=7410093306en_HK
dc.identifier.scopusauthoridTang, ZY=7403306244en_HK
dc.identifier.scopusauthoridChen, SS=7410262848en_HK

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