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Article: Feasibility of one-shot-per-crystal structure determination using Laue diffraction

TitleFeasibility of one-shot-per-crystal structure determination using Laue diffraction
Authors
KeywordsLaue diffraction
Microcrystallography
X-ray optics
Issue Date2010
PublisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1
Citation
Acta Crystallographica Section D: Biological Crystallography, 2010, v. 66 n. 1, p. 2-11 How to Cite?
AbstractCrystal size is an important factor in determining the number of diffraction patterns which may be obtained from a protein crystal before severe radiation damage sets in. As crystal dimensions decrease this number is reduced, eventually falling to one, at which point a complete data set must be assembled using data from multiple crystals. When only a single exposure is to be collected from each crystal, the polychromatic Laue technique may be preferable to monochromatic methods owing to its simultaneous recording of a large number of fully recorded reflections per image. To assess the feasibility of solving structures using single Laue images from multiple crystals, data were collected using a 'pink' beam at the CHESS D1 station from groups of lysozyme crystals with dimensions of the order of 20-30 m mounted on MicroMesh grids. Single-shot Laue data were used for structure determination by molecular replacement and correct solutions were obtained even when as few as five crystals were used. © 2010 International Union of Crystallography Printed in Singapore - all rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/65603
ISSN
2013 Impact Factor: 7.232
2015 SCImago Journal Rankings: 3.088
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
National Science Foundation
National Institutes of Health/National Institute of General Medical SciencesDMR-0225180
National Institutes of HealthRR-01646
Funding Information:

This work is based upon research conducted at the Cornell High Energy Synchrotron Source (CHESS), which is supported by the National Science Foundation and the National Institutes of Health/National Institute of General Medical Sciences under NSF award DMR-0225180, using the Macromolecular Diffraction at CHESS (MacCHESS) facility, which is supported by award RR-01646 from the National Institutes of Health through its National Center for Research Resources. We are very grateful to the staffs of CHESS and MacCHESS, particularly Bill Miller, Mike Cook and Scott Smith, for assistance in setting up the complex equipment needed to carry out the Laue experiment. We also wish to thank the anonymous reviewers of this paper for their very detailed and constructive comments, which led to a much improved final version.

References

 

DC FieldValueLanguage
dc.contributor.authorCornaby, Sen_HK
dc.contributor.authorSzebenyi, DMEen_HK
dc.contributor.authorSmilgies, DMen_HK
dc.contributor.authorSchuller, DJen_HK
dc.contributor.authorGillilan, Ren_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorBilderback, DHen_HK
dc.date.accessioned2010-09-03T07:28:52Z-
dc.date.available2010-09-03T07:28:52Z-
dc.date.issued2010en_HK
dc.identifier.citationActa Crystallographica Section D: Biological Crystallography, 2010, v. 66 n. 1, p. 2-11en_HK
dc.identifier.issn0907-4449en_HK
dc.identifier.urihttp://hdl.handle.net/10722/65603-
dc.description.abstractCrystal size is an important factor in determining the number of diffraction patterns which may be obtained from a protein crystal before severe radiation damage sets in. As crystal dimensions decrease this number is reduced, eventually falling to one, at which point a complete data set must be assembled using data from multiple crystals. When only a single exposure is to be collected from each crystal, the polychromatic Laue technique may be preferable to monochromatic methods owing to its simultaneous recording of a large number of fully recorded reflections per image. To assess the feasibility of solving structures using single Laue images from multiple crystals, data were collected using a 'pink' beam at the CHESS D1 station from groups of lysozyme crystals with dimensions of the order of 20-30 m mounted on MicroMesh grids. Single-shot Laue data were used for structure determination by molecular replacement and correct solutions were obtained even when as few as five crystals were used. © 2010 International Union of Crystallography Printed in Singapore - all rights reserved.en_HK
dc.languageeng-
dc.publisherWiley-Blackwell Publishing, Inc.. The Journal's web site is located at http://www.wiley.com/bw/editors.asp?ref=0907-4449&site=1en_HK
dc.relation.ispartofActa Crystallographica Section D: Biological Crystallographyen_HK
dc.subjectLaue diffractionen_HK
dc.subjectMicrocrystallographyen_HK
dc.subjectX-ray opticsen_HK
dc.subject.meshCrystallization-
dc.subject.meshCrystallography, X-Ray - instrumentation - methods-
dc.subject.meshFeasibility Studies-
dc.subject.meshMuramidase - chemistry - metabolism-
dc.subject.meshProtein Conformation-
dc.titleFeasibility of one-shot-per-crystal structure determination using Laue diffractionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0907-4449&volume=66&issue=1&spage=2&epage=11&date=2010&atitle=Feasibility+of+one-shot-per-crystal+structure+determination+using+Laue+diffraction-
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1107/S0907444909037731en_HK
dc.identifier.pmid20057043-
dc.identifier.pmcidPMC2803125-
dc.identifier.scopuseid_2-s2.0-74549211343en_HK
dc.identifier.hkuros170424-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-74549211343&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume66en_HK
dc.identifier.issue1en_HK
dc.identifier.spage2en_HK
dc.identifier.epage11en_HK
dc.identifier.isiWOS:000273758800002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridCornaby, S=15831307900en_HK
dc.identifier.scopusauthoridSzebenyi, DME=6603230617en_HK
dc.identifier.scopusauthoridSmilgies, DM=7003298142en_HK
dc.identifier.scopusauthoridSchuller, DJ=7102716051en_HK
dc.identifier.scopusauthoridGillilan, R=6701426897en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridBilderback, DH=7004586474en_HK
dc.identifier.citeulike6465047-

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