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Article: Functional characterization of THY1 as a tumor suppressor gene with antiinvasive activity in nasopharyngeal carcinoma
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TitleFunctional characterization of THY1 as a tumor suppressor gene with antiinvasive activity in nasopharyngeal carcinoma
 
AuthorsLung, HL2 5
Cheung, AKL2 5
Cheng, Y5 4
Kwong, FM2 5
Lo, PHY2 5
Law, EWL2 5
Chua, D2
Zabarovsky, ER1
Wang, N3
Tsao, SW2
Stanbridge, EJ6
Lung, ML2 5
 
KeywordsAntiinvasive
Nasopharyngeal carcinoma
Tetracycline-regulated gene expression
THY1
Tumor suppressor gene
 
Issue Date2010
 
PublisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
 
CitationInternational Journal Of Cancer, 2010, v. 127 n. 2, p. 304-312 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ijc.25047
 
AbstractTHY1 was previously identified as a candidate tumor suppressor gene (TSG) associated with lymph node metastases in nasopharyngeal carcinoma (NPC) through functional studies. It was identified by oligonucleotide microarray analysis as an interesting differentially expressed gene. However, direct functional evidence is still lacking for THY1 being a TSG in NPC, as in vivo tumorigenicity assays have not been previously reported in our last study of THY1. In this study, a tetracycline-inducible expression vector, pETE-Bsd, was used to obtain stable transfectants of THY1. The stringent in vivo tumorigenicity assay results show that the activation of THY1 suppresses tumor formation of HONE1 cells in nude mice, and the tumor formation ability was restored in the presence of doxycycline (a tetracycline analog), when the gene is shut off. Functional inactivation of this gene is observed in all the tumors derived from the tumorigenic transfectant. The tumor suppressive effect could be repressed by knockdown of THY1 expression in nontumorigenic microcell hybrids. Further studies indicate that expression of THY1 inhibits HONE1 cell growth in vitro by arresting cells in G0/G1 phase. It greatly reduces the ability for anchorage-independent growth. The invasiveness of HONE1 cells was also inhibited by the expression of THY1. These findings suggest that THY1 is a TSG in NPC, which is involved in invasion and shows an association with tumor metastasis. Taken together, THY1 clearly plays an important functional role in tumor suppression in NPC. © 2009 UICC.
 
ISSN0020-7136
2012 Impact Factor: 6.198
2012 SCImago Journal Rankings: 2.309
 
DOIhttp://dx.doi.org/10.1002/ijc.25047
 
ISI Accession Number IDWOS:000278919000006
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, People's Republic of ChinaCA03/04.SC01
Swedish Cancer Society
Swedish Research Council
Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
Swedish Institute
Royal Swedish Academy of Sciences
INTAS
Karolinska Institute
Funding Information:

Grant sponsor: Research Grants Council of the Hong Kong Special Administrative Region, People's Republic of China; Grant number: CA03/04.SC01; Grant sponsors: the Swedish Cancer Society, the Swedish Research Council, the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), the Swedish Institute, the Royal Swedish Academy of Sciences, INTAS, Karolinska Institute

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorLung, HL
 
dc.contributor.authorCheung, AKL
 
dc.contributor.authorCheng, Y
 
dc.contributor.authorKwong, FM
 
dc.contributor.authorLo, PHY
 
dc.contributor.authorLaw, EWL
 
dc.contributor.authorChua, D
 
dc.contributor.authorZabarovsky, ER
 
dc.contributor.authorWang, N
 
dc.contributor.authorTsao, SW
 
dc.contributor.authorStanbridge, EJ
 
dc.contributor.authorLung, ML
 
dc.date.accessioned2010-08-10T06:26:24Z
 
dc.date.available2010-08-10T06:26:24Z
 
dc.date.issued2010
 
dc.description.abstractTHY1 was previously identified as a candidate tumor suppressor gene (TSG) associated with lymph node metastases in nasopharyngeal carcinoma (NPC) through functional studies. It was identified by oligonucleotide microarray analysis as an interesting differentially expressed gene. However, direct functional evidence is still lacking for THY1 being a TSG in NPC, as in vivo tumorigenicity assays have not been previously reported in our last study of THY1. In this study, a tetracycline-inducible expression vector, pETE-Bsd, was used to obtain stable transfectants of THY1. The stringent in vivo tumorigenicity assay results show that the activation of THY1 suppresses tumor formation of HONE1 cells in nude mice, and the tumor formation ability was restored in the presence of doxycycline (a tetracycline analog), when the gene is shut off. Functional inactivation of this gene is observed in all the tumors derived from the tumorigenic transfectant. The tumor suppressive effect could be repressed by knockdown of THY1 expression in nontumorigenic microcell hybrids. Further studies indicate that expression of THY1 inhibits HONE1 cell growth in vitro by arresting cells in G0/G1 phase. It greatly reduces the ability for anchorage-independent growth. The invasiveness of HONE1 cells was also inhibited by the expression of THY1. These findings suggest that THY1 is a TSG in NPC, which is involved in invasion and shows an association with tumor metastasis. Taken together, THY1 clearly plays an important functional role in tumor suppression in NPC. © 2009 UICC.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationInternational Journal Of Cancer, 2010, v. 127 n. 2, p. 304-312 [How to Cite?]
DOI: http://dx.doi.org/10.1002/ijc.25047
 
dc.identifier.doihttp://dx.doi.org/10.1002/ijc.25047
 
dc.identifier.eissn1097-0215
 
dc.identifier.epage312
 
dc.identifier.hkuros173235
 
dc.identifier.hkuros168362
 
dc.identifier.hkuros186156
 
dc.identifier.hkuros186345
 
dc.identifier.isiWOS:000278919000006
Funding AgencyGrant Number
Research Grants Council of the Hong Kong Special Administrative Region, People's Republic of ChinaCA03/04.SC01
Swedish Cancer Society
Swedish Research Council
Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
Swedish Institute
Royal Swedish Academy of Sciences
INTAS
Karolinska Institute
Funding Information:

Grant sponsor: Research Grants Council of the Hong Kong Special Administrative Region, People's Republic of China; Grant number: CA03/04.SC01; Grant sponsors: the Swedish Cancer Society, the Swedish Research Council, the Swedish Foundation for International Cooperation in Research and Higher Education (STINT), the Swedish Institute, the Royal Swedish Academy of Sciences, INTAS, Karolinska Institute

 
dc.identifier.issn0020-7136
2012 Impact Factor: 6.198
2012 SCImago Journal Rankings: 2.309
 
dc.identifier.issue2
 
dc.identifier.openurl
 
dc.identifier.pmid19921696
 
dc.identifier.scopuseid_2-s2.0-77953709847
 
dc.identifier.spage304
 
dc.identifier.urihttp://hdl.handle.net/10722/65479
 
dc.identifier.volume127
 
dc.languageeng
 
dc.publisherJohn Wiley & Sons, Inc.. The Journal's web site is located at http://www3.interscience.wiley.com/journal/29331/home
 
dc.publisher.placeUnited States
 
dc.relation.ispartofInternational Journal of Cancer
 
dc.relation.referencesReferences in Scopus
 
dc.rightsInternational Journal of Cancer. Copyright © John Wiley & Sons, Inc.
 
dc.subject.meshAntigens, Thy-1 - physiology
 
dc.subject.meshCell Movement
 
dc.subject.meshGene Expression Regulation, Neoplastic - physiology
 
dc.subject.meshGenes, Tumor Suppressor - physiology
 
dc.subject.meshNasopharyngeal Neoplasms - genetics - metabolism - pathology
 
dc.subjectAntiinvasive
 
dc.subjectNasopharyngeal carcinoma
 
dc.subjectTetracycline-regulated gene expression
 
dc.subjectTHY1
 
dc.subjectTumor suppressor gene
 
dc.titleFunctional characterization of THY1 as a tumor suppressor gene with antiinvasive activity in nasopharyngeal carcinoma
 
dc.typeArticle
 
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<contributor.author>Chua, D</contributor.author>
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Author Affiliations
  1. Karolinska University Hospital
  2. The University of Hong Kong
  3. University of Rochester School of Medicine and Dentistry
  4. City of Hope National Med Center
  5. Hong Kong University of Science and Technology
  6. UC Irvine