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Article: Detection of SARS coronavirus in humans and animals by conventional and quantitative (real time) reverse transcription polymerase chain reactions

TitleDetection of SARS coronavirus in humans and animals by conventional and quantitative (real time) reverse transcription polymerase chain reactions
Authors
KeywordsClinical diagnosis
Molecular detection
RT-PCR
SARS coronavirus
Issue Date2008
PublisherJohn Wiley & Sons, Inc.
Citation
Methods In Molecular Biology, 2008, v. 454, p. 61-72 How to Cite?
AbstractSevere acute respiratory syndrome is a novel human disease caused by a coronavirus of animal origin. Soon after the discovery SARS-CoV, several molecular assays were described for the detection of this virus. Of these, conventional and quantitative RT-PCR approaches were the primary tools for SARS-CoV RNA detection. In this chapter we describe a two-step conventional RT-PCR and a one-step quantitative RT-PCR that were used routinely in our laboratories during the SARS outbreak. © 2008 Humana Press.
Persistent Identifierhttp://hdl.handle.net/10722/64532
ISSN
2015 SCImago Journal Rankings: 0.549
References

 

DC FieldValueLanguage
dc.contributor.authorPeiris, JSMen_HK
dc.contributor.authorPoon, LLMen_HK
dc.date.accessioned2010-07-13T04:53:14Z-
dc.date.available2010-07-13T04:53:14Z-
dc.date.issued2008en_HK
dc.identifier.citationMethods In Molecular Biology, 2008, v. 454, p. 61-72en_HK
dc.identifier.issn1064-3745en_HK
dc.identifier.urihttp://hdl.handle.net/10722/64532-
dc.description.abstractSevere acute respiratory syndrome is a novel human disease caused by a coronavirus of animal origin. Soon after the discovery SARS-CoV, several molecular assays were described for the detection of this virus. Of these, conventional and quantitative RT-PCR approaches were the primary tools for SARS-CoV RNA detection. In this chapter we describe a two-step conventional RT-PCR and a one-step quantitative RT-PCR that were used routinely in our laboratories during the SARS outbreak. © 2008 Humana Press.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc.en_HK
dc.relation.ispartofMethods in Molecular Biologyen_HK
dc.subjectClinical diagnosisen_HK
dc.subjectMolecular detectionen_HK
dc.subjectRT-PCRen_HK
dc.subjectSARS coronavirusen_HK
dc.subject.meshAnimals-
dc.subject.meshHumans-
dc.subject.meshRNA, Viral - genetics - isolation and purification-
dc.subject.meshReverse Transcriptase Polymerase Chain Reaction - methods-
dc.subject.meshSARS Virus - genetics - isolation and purification-
dc.titleDetection of SARS coronavirus in humans and animals by conventional and quantitative (real time) reverse transcription polymerase chain reactionsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0898-7750&volume=454&spage=61&epage=72&date=2008&atitle=Detection+of+SARS+coronavirus+in+humans+and+animals+by+conventional+and+quantitative+(real+time)+reverse+transcription+polymerase+chain+reactions.en_HK
dc.identifier.emailPeiris, JSM: malik@hkucc.hku.hken_HK
dc.identifier.emailPoon, LLM: llmpoon@hkucc.hku.hken_HK
dc.identifier.authorityPeiris, JSM=rp00410en_HK
dc.identifier.authorityPoon, LLM=rp00484en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/978-1-59745-181-9-6en_HK
dc.identifier.pmid19057863-
dc.identifier.scopuseid_2-s2.0-62449089954en_HK
dc.identifier.hkuros164362en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62449089954&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume454en_HK
dc.identifier.spage61en_HK
dc.identifier.epage72en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridPeiris, JSM=7005486823en_HK
dc.identifier.scopusauthoridPoon, LLM=7005441747en_HK

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