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Article: Effects of remifentanil on intracellular Ca2+ and its transients induced by electrical stimulation and caffeine in rat ventricular myocytes

TitleEffects of remifentanil on intracellular Ca2+ and its transients induced by electrical stimulation and caffeine in rat ventricular myocytes
Authors
Zhang, YIrwin, MGLi, RChen, ZWWong, TM
KeywordsCytosolic-calcium
Opioid receptor
Remifentanil
Ventricular myocyte
Issue Date2009
PublisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/
Citation
Chinese Medical Journal, 2009, v. 122 n. 12, p. 1439-1443 How to Cite?
DOI: http://dx.doi.org/10.3760/cma.j.issn.0366-6999.2009.12.016
AbstractBackground: Preconditioning with remifentanil confers cardioprotection. Since Ca2+ overload is a precipitating factor of injury, we determined the effects of remefentanil on intracellular Ca2+ ([Ca2+]i) and its transients induced by electrical stimulation and caffeine, which reflects Ca2+ handling by Ca2+ handling proteins, in rat ventricular myocytes. Methods: Freshly isolated adult male Sprague-Dawley rat myocytes were loaded with Fura-2/AM and [Ca]i was determined by spectrofluorometry. Remifentanil at 0.1-1000 μg/L was administered. Ten minutes after administration, either 0.2 Hz electrical stimulation was applied or 10 mmol/L caffeine was added. The [Ca2+]i, and the amplitude, time resting and 50% decay (t50) of both transients induced by electrical stimulation (E[Ca2+]i) and caffeine (C[Ca2+]i) were determined. Results: Remifentanil (0.1-1000.0 μg/L) decreased the [Ca2+]i in a dose-dependent manner. It also decreased the amplitude of both transients dose-dependently. Furthermore, it increased the time to peak and t50 of both transients dose-dependently. Conclusion: Remifentanil reduced the [Ca2+]i and suppressed the transients induced by electrical stimulation and caffeine in rat ventricular myocytes.
Persistent Identifierhttp://hdl.handle.net/10722/63422
ISSN
0366-6999
2021 Impact Factor: 6.133
2020 SCImago Journal Rankings: 0.537
ISI Accession Number ID
WOS:000267615200016
Funding AgencyGrant Number
National Natural Science Foundation of China30672032
Excellent Youth Foundation of Anhui Scientific Committee08040106814
Funding Information:

This study was supported by grants from the National Natural Science Foundation of China (No. 30672032) and the Excellent Youth Foundation of Anhui Scientific Committee (No. 08040106814).

References
References in Scopus

 

DC FieldValueLanguage
dc.contributor.authorZhang, Yen_HK
dc.contributor.authorIrwin, MGen_HK
dc.contributor.authorLi, Ren_HK
dc.contributor.authorChen, ZWen_HK
dc.contributor.authorWong, TMen_HK
dc.date.accessioned2010-07-13T04:23:17Z-
dc.date.available2010-07-13T04:23:17Z-
dc.date.issued2009en_HK
dc.identifier.citationChinese Medical Journal, 2009, v. 122 n. 12, p. 1439-1443en_HK
dc.identifier.issn0366-6999en_HK
dc.identifier.urihttp://hdl.handle.net/10722/63422-
dc.description.abstractBackground: Preconditioning with remifentanil confers cardioprotection. Since Ca2+ overload is a precipitating factor of injury, we determined the effects of remefentanil on intracellular Ca2+ ([Ca2+]i) and its transients induced by electrical stimulation and caffeine, which reflects Ca2+ handling by Ca2+ handling proteins, in rat ventricular myocytes. Methods: Freshly isolated adult male Sprague-Dawley rat myocytes were loaded with Fura-2/AM and [Ca]i was determined by spectrofluorometry. Remifentanil at 0.1-1000 μg/L was administered. Ten minutes after administration, either 0.2 Hz electrical stimulation was applied or 10 mmol/L caffeine was added. The [Ca2+]i, and the amplitude, time resting and 50% decay (t50) of both transients induced by electrical stimulation (E[Ca2+]i) and caffeine (C[Ca2+]i) were determined. Results: Remifentanil (0.1-1000.0 μg/L) decreased the [Ca2+]i in a dose-dependent manner. It also decreased the amplitude of both transients dose-dependently. Furthermore, it increased the time to peak and t50 of both transients dose-dependently. Conclusion: Remifentanil reduced the [Ca2+]i and suppressed the transients induced by electrical stimulation and caffeine in rat ventricular myocytes.en_HK
dc.languageengen_HK
dc.publisherChinese Medical Association. The Journal's web site is located at http://www.cmj.org/en_HK
dc.relation.ispartofChinese Medical Journalen_HK
dc.subjectCytosolic-calcium-
dc.subjectOpioid receptor-
dc.subjectRemifentanil-
dc.subjectVentricular myocyte-
dc.subject.meshAnimalsen_HK
dc.subject.meshCaffeine - pharmacologyen_HK
dc.subject.meshCalcium - metabolismen_HK
dc.subject.meshCalcium Signaling - drug effectsen_HK
dc.subject.meshCells, Cultureden_HK
dc.subject.meshElectric Stimulationen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMyocytes, Cardiac - drug effects - metabolismen_HK
dc.subject.meshPiperidines - pharmacologyen_HK
dc.subject.meshRatsen_HK
dc.subject.meshRats, Sprague-Dawleyen_HK
dc.titleEffects of remifentanil on intracellular Ca2+ and its transients induced by electrical stimulation and caffeine in rat ventricular myocytesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0578-1337&volume=122&issue=12&spage=1439&epage=1443&date=2009&atitle=Effects+of+remifentanil+on+intracellular+Ca2++and+its+transients+induced+by+electrical+stimulation+and+caffeine+in+rat+ventricular+myocytesen_HK
dc.identifier.emailIrwin, MG:mgirwin@hku.hken_HK
dc.identifier.authorityIrwin, MG=rp00390en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.3760/cma.j.issn.0366-6999.2009.12.016en_HK
dc.identifier.pmid19567168-
dc.identifier.scopuseid_2-s2.0-68349097409en_HK
dc.identifier.hkuros160868en_HK
dc.identifier.hkuros177949-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68349097409&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume122en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1439en_HK
dc.identifier.epage1443en_HK
dc.identifier.isiWOS:000267615200016-
dc.publisher.placeChinaen_HK
dc.identifier.issnl0366-6999-

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