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Conference Paper: Possible role of organic cation transport-3 in serotonin uptake in human brain vascular smooth muscle cells

TitlePossible role of organic cation transport-3 in serotonin uptake in human brain vascular smooth muscle cells
Authors
Issue Date2009
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR
Citation
The 10th International Symposium on Mechanisms of Vasodilatation (MOVD 2009), Matsushima, Miyagi, Japan, 1-3 June 2009. In Journal of Vascular Research, 2009, v. 46 suppl. 1, p. 202, abstract no. P17-6 How to Cite?
AbstractSerotonin (5HT) is a vasoconstrictor. It has been reported that 5HT can be taken up by vascular smooth muscle cells of rat aortas through the serotonin transporters (SERT). 7KLV +7XSWDNHPHFKDQLVPSOD\VDFUXFLDOUROHLQ¿QH WXQLQJWKHDYDLODELOLW\RI +7DW its cognate receptors. However, it is unclear if SERT or other transporters are responsible for the 5HT uptake in vascular smooth muscle cells of human resistance arteries. The aim of this work was to characterize the 5HT uptake in human brain vascular smooth muscle cells (HBVSMCs). The [3 H]5HT uptake in HBVSMCs was increased with time and was saturable with a Michaelis-menten constant of 50.36 ± 10.2 mM. The [3 H]5HT uptake was enhanced when the extracellular medium was changed alkaline. Moreover, the [3 H]5HT XSWDNHZDVUHVLVWDQWWRFLWDORSUDP D6(57LQKLELWRU XSWR ȝ0 EXWLWFRXOGEHLQKLELWHG by citalopram at higher concentrations (nearly 40% inhibition by 1 mM). Tetraammonium (TEA, at 1 mM) and 1-methy-4-phenylpyridinium (MPP, at 1 mM), which are substrates of organic cation transporters (OCTs), inhibited 5HT uptake by 17 and 26%, respectively. The ORZDI¿QLW\RI +7WUDQVSRUW S+GHSHQGHQFHDQGLQKLELWLRQE\RUJDQLFFDWLRQVDUHW\SLFDO characteristics of OCTs. In addition, the results of RT-PCR demonstrated the presence of mRNA of OCT-3, but the absence of OCT-1, OCT-2, organic anion transporters (OATs) and SERT. Therefore, we suggest that the 5HT uptake in HBVSMCs is different from that in rat aorta. It is partly mediated by OCT-3, but does not involve SERT.
DescriptionThis free access journal suppl. is Proceedings of the 10th International Symposium on Mechanisms of Vasodilatation 2009
Persistent Identifierhttp://hdl.handle.net/10722/62848
ISSN
2015 Impact Factor: 2.186
2015 SCImago Journal Rankings: 1.119

 

DC FieldValueLanguage
dc.contributor.authorHo, YWen_HK
dc.contributor.authorLi, WSRen_HK
dc.contributor.authorVanhoutte, PMGRen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorLeung, SWSen_HK
dc.contributor.authorLeung, GPHen_HK
dc.date.accessioned2010-07-13T04:10:31Z-
dc.date.available2010-07-13T04:10:31Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 10th International Symposium on Mechanisms of Vasodilatation (MOVD 2009), Matsushima, Miyagi, Japan, 1-3 June 2009. In Journal of Vascular Research, 2009, v. 46 suppl. 1, p. 202, abstract no. P17-6-
dc.identifier.issn1018-1172-
dc.identifier.urihttp://hdl.handle.net/10722/62848-
dc.descriptionThis free access journal suppl. is Proceedings of the 10th International Symposium on Mechanisms of Vasodilatation 2009-
dc.description.abstractSerotonin (5HT) is a vasoconstrictor. It has been reported that 5HT can be taken up by vascular smooth muscle cells of rat aortas through the serotonin transporters (SERT). 7KLV +7XSWDNHPHFKDQLVPSOD\VDFUXFLDOUROHLQ¿QH WXQLQJWKHDYDLODELOLW\RI +7DW its cognate receptors. However, it is unclear if SERT or other transporters are responsible for the 5HT uptake in vascular smooth muscle cells of human resistance arteries. The aim of this work was to characterize the 5HT uptake in human brain vascular smooth muscle cells (HBVSMCs). The [3 H]5HT uptake in HBVSMCs was increased with time and was saturable with a Michaelis-menten constant of 50.36 ± 10.2 mM. The [3 H]5HT uptake was enhanced when the extracellular medium was changed alkaline. Moreover, the [3 H]5HT XSWDNHZDVUHVLVWDQWWRFLWDORSUDP D6(57LQKLELWRU XSWR ȝ0 EXWLWFRXOGEHLQKLELWHG by citalopram at higher concentrations (nearly 40% inhibition by 1 mM). Tetraammonium (TEA, at 1 mM) and 1-methy-4-phenylpyridinium (MPP, at 1 mM), which are substrates of organic cation transporters (OCTs), inhibited 5HT uptake by 17 and 26%, respectively. The ORZDI¿QLW\RI +7WUDQVSRUW S+GHSHQGHQFHDQGLQKLELWLRQE\RUJDQLFFDWLRQVDUHW\SLFDO characteristics of OCTs. In addition, the results of RT-PCR demonstrated the presence of mRNA of OCT-3, but the absence of OCT-1, OCT-2, organic anion transporters (OATs) and SERT. Therefore, we suggest that the 5HT uptake in HBVSMCs is different from that in rat aorta. It is partly mediated by OCT-3, but does not involve SERT.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR-
dc.relation.ispartofJournal of Vascular Research-
dc.titlePossible role of organic cation transport-3 in serotonin uptake in human brain vascular smooth muscle cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailHo, YW: eywho@graduate.hku.hken_HK
dc.identifier.emailVanhoutte, PMGR: vanhoutt@hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailLeung, SWS: swsleung@hkucc.hku.hken_HK
dc.identifier.emailLeung, GPH: gphleung@hkucc.hku.hken_HK
dc.identifier.authorityVanhoutte, PMGR=rp00238en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityLeung, SWS=rp00235en_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1159/000222318-
dc.identifier.hkuros156242en_HK
dc.identifier.hkuros157520-

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