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Conference Paper: Rapid, non-genomic vascular actions of genistein involves a G-protein coupled receptor

TitleRapid, non-genomic vascular actions of genistein involves a G-protein coupled receptor
Authors
Issue Date2009
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR
Citation
The 10th International Symposium on Mechanisms of Vasodilatation (MOVD 2009), Matsushima, Miyagi, Japan, 1-3 June 2009. In Journal of Vascular Research, 2009, v. 46 suppl. 1, p. 187, abstract no. P15-5 How to Cite?
AbstractBackground: Genistein enhances endothelial function in a receptor-mediated manner. The present studies were designed to identify the putative receptor and related signaling pathways in the rapid vascular actions of genistein. Methods: Isometric tension was measured in isolated aortic rings from 32-weeksROGPDOHVSRQWDQHRXVO\K\SHUWHQVLYH UDWV (VWURJHQ UHFHSWRU Į HVWURJHQ UHFHSWRU Į HVWURJHQ UHFHSWRU Į DQG* SURWHLQ FRXSOHG UHFHSWRU LGHQWLILHG DVWKH SRWHQWLDO candidates of the putative receptor, were cloned and expressed using a cell-free expression system (MembraneMax Protein Expression Kit). Results: Genistein acutely potentiated acetylcholine-induced relaxation. This effect was insensitive to the transcription and translation inhibitors, actinomycin D and cycloheximide respectively. The potentiation of acetylcholine and A23187-induced relaxation by genistein ZDVLQKLELWHG E\1) DQG*3 DQWDJRQLVW $ WKH VHOHFWLYH*i/o and Gq Į VXEXQLW DQWDJRQLVWV UHVSHFWLYHO\ EXWQRWE\1) DVHOHFWLYH*sĮ VXEXQLWDQWDJRQLVW ,QRUGHU to identify the putative receptors, the cDNA of candidates were successfully amplified IURP0&) FHOOXVLQJ3&5DQGIXUWKHUFORQHGLQWRWKHH[SUHVVLRQYHFWRU S(;3 17 7232 ZKLFKZDVFRQ¿UPHGE\VHTXHQFLQJ 6ROXEOHUHFHSWRUSURWHLQVZHUHH[SUHVVHGin vitro ZKRVHPROHFXODUVL]HVZHUHFRQ¿UPHGE\:HVWHUQEORWWLQJDQGSXUL¿HG IRUXVHLQ radioligand binding assay. Conclusion: Our results demonstrate that rapid effect of genistein in potentiating UHOD[DWLRQLVQRQ JHQRPLFDQG*Įi DQG*Įq EXWQRW*Įs, were involved in the effect of genistein in potentiating acetylcholine and A23187-induced relaxations. Involvement of G-proteins suggests that genistein exerts its effect through a putative G-protein coupled receptor. Radioligand binding assays will be performed to confirm the identity of this putative receptor.
DescriptionThis free access journal suppl. is Proceedings of the 10th International Symposium on Mechanisms of Vasodilatation 2009
Persistent Identifierhttp://hdl.handle.net/10722/62842
ISSN
2015 Impact Factor: 2.186
2015 SCImago Journal Rankings: 1.119

 

DC FieldValueLanguage
dc.contributor.authorLin, HYAen_HK
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorLeung, SWSen_HK
dc.contributor.authorMan, RYKen_HK
dc.date.accessioned2010-07-13T04:10:24Z-
dc.date.available2010-07-13T04:10:24Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 10th International Symposium on Mechanisms of Vasodilatation (MOVD 2009), Matsushima, Miyagi, Japan, 1-3 June 2009. In Journal of Vascular Research, 2009, v. 46 suppl. 1, p. 187, abstract no. P15-5-
dc.identifier.issn1018-1172-
dc.identifier.urihttp://hdl.handle.net/10722/62842-
dc.descriptionThis free access journal suppl. is Proceedings of the 10th International Symposium on Mechanisms of Vasodilatation 2009-
dc.description.abstractBackground: Genistein enhances endothelial function in a receptor-mediated manner. The present studies were designed to identify the putative receptor and related signaling pathways in the rapid vascular actions of genistein. Methods: Isometric tension was measured in isolated aortic rings from 32-weeksROGPDOHVSRQWDQHRXVO\K\SHUWHQVLYH UDWV (VWURJHQ UHFHSWRU Į HVWURJHQ UHFHSWRU Į HVWURJHQ UHFHSWRU Į DQG* SURWHLQ FRXSOHG UHFHSWRU LGHQWLILHG DVWKH SRWHQWLDO candidates of the putative receptor, were cloned and expressed using a cell-free expression system (MembraneMax Protein Expression Kit). Results: Genistein acutely potentiated acetylcholine-induced relaxation. This effect was insensitive to the transcription and translation inhibitors, actinomycin D and cycloheximide respectively. The potentiation of acetylcholine and A23187-induced relaxation by genistein ZDVLQKLELWHG E\1) DQG*3 DQWDJRQLVW $ WKH VHOHFWLYH*i/o and Gq Į VXEXQLW DQWDJRQLVWV UHVSHFWLYHO\ EXWQRWE\1) DVHOHFWLYH*sĮ VXEXQLWDQWDJRQLVW ,QRUGHU to identify the putative receptors, the cDNA of candidates were successfully amplified IURP0&) FHOOXVLQJ3&5DQGIXUWKHUFORQHGLQWRWKHH[SUHVVLRQYHFWRU S(;3 17 7232 ZKLFKZDVFRQ¿UPHGE\VHTXHQFLQJ 6ROXEOHUHFHSWRUSURWHLQVZHUHH[SUHVVHGin vitro ZKRVHPROHFXODUVL]HVZHUHFRQ¿UPHGE\:HVWHUQEORWWLQJDQGSXUL¿HG IRUXVHLQ radioligand binding assay. Conclusion: Our results demonstrate that rapid effect of genistein in potentiating UHOD[DWLRQLVQRQ JHQRPLFDQG*Įi DQG*Įq EXWQRW*Įs, were involved in the effect of genistein in potentiating acetylcholine and A23187-induced relaxations. Involvement of G-proteins suggests that genistein exerts its effect through a putative G-protein coupled receptor. Radioligand binding assays will be performed to confirm the identity of this putative receptor.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR-
dc.relation.ispartofJournal of Vascular Research-
dc.titleRapid, non-genomic vascular actions of genistein involves a G-protein coupled receptoren_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLeung, GPH: leung_pak_heng@hotmail.comen_HK
dc.identifier.emailLeung, SWS: swsleung@HKUCC.hku.hken_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.identifier.authorityLeung, SWS=rp00235en_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1159/000222318-
dc.identifier.hkuros156239en_HK
dc.identifier.volume46-
dc.identifier.issuesuppl. 1-
dc.identifier.spage187, abstract no. P15-5-
dc.identifier.epage187, abstract no. P15-5-

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