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Conference Paper: Diabetes- and hypertension-induced upregulation of concentrative nucleoside transporter-2 in endothelial cells

TitleDiabetes- and hypertension-induced upregulation of concentrative nucleoside transporter-2 in endothelial cells
Authors
Issue Date2009
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVR
Citation
The 10th International Symposium on Mechanisms of Vasodilatation (MOVD 2009), Matsushima, Miyagi, Japan, 1-3 June 2009. In Journal of Vascular Research, 2009, v. 46 suppl. 1, p. 178, abstract no. P14-4 How to Cite?
AbstractAdenosine modulates a variety of vascular functions and adenosine homeostasis is controlled by nucleoside transporters. Nucleoside transporters are divided into 2 classes. The equilibrative nucleoside transporters (ENTs) are Na+ -independent and are VXEGLYLGHGLQWR W\SHVEDVHGRQWKHLUVHQVLWLYLWLHVWR > QLWUREHQ]\O WKLRO@ ȕ ' ribofuranosylpurine (NBMPR). The concentrative nucleoside transporters (CNTs) are Na+ -dependent and are subdivided into 3 types based on the substrate selectivity. In this study, the nucleoside transporters in vascular cells were characterized and their changes in diabetes and hypertension were investigated. )XQFWLRQDOVWXGLHVVKRZHGWKDW RIWKH>3 H]adenosine transport in human brain vascular endothelial cells (HBECs) was Na+ -dependent. In the Na+ -independent component, 80% was inhibited by NBMPR (10 nM, which inhibits ENT1 but not ENT2). However, the [3 H]adenosine transport in vascular smooth muscle cells (HBSMCs) was totally Na+ -independent and 95% of it was inhibited by NBMPR (10 nM). RT-PCR revealed the presence of mRNA of ENT1, ENT2 and CNT2 in HBECs, but no CNT2 was found in HBSMCs. The unique presence of CNT2 in endothelial cells was confirmed by the observation that mRNA of ENT1, ENT2, and CNT2 were present in rat basilar arteries but no CNT2 was detected after removal of endothelia. The mRNA expression of CNT2, but not ENT1 and ENT2, was higher in basilar arteries of spontaneously hypertensive rats and streptozotocin-induced diabetic rats compared with those in normal rats. Such increase in CNT2 in hypertension and diabetes may affect the availability of adenosine in the vicinity of adenosine receptors of endothelial cells and, thus, alter vascular functions.
DescriptionThis free access journal suppl. is Proceedings of the 10th International Symposium on Mechanisms of Vasodilatation 2009
Persistent Identifierhttp://hdl.handle.net/10722/62821
ISSN
2015 Impact Factor: 2.186
2015 SCImago Journal Rankings: 1.119

 

DC FieldValueLanguage
dc.contributor.authorLeung, GPHen_HK
dc.contributor.authorHo, YWen_HK
dc.contributor.authorLi, WSRen_HK
dc.contributor.authorSeto, SWen_HK
dc.contributor.authorKwan, YWen_HK
dc.date.accessioned2010-07-13T04:09:57Z-
dc.date.available2010-07-13T04:09:57Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 10th International Symposium on Mechanisms of Vasodilatation (MOVD 2009), Matsushima, Miyagi, Japan, 1-3 June 2009. In Journal of Vascular Research, 2009, v. 46 suppl. 1, p. 178, abstract no. P14-4en_HK
dc.identifier.issn1018-1172en_HK
dc.identifier.urihttp://hdl.handle.net/10722/62821-
dc.descriptionThis free access journal suppl. is Proceedings of the 10th International Symposium on Mechanisms of Vasodilatation 2009-
dc.description.abstractAdenosine modulates a variety of vascular functions and adenosine homeostasis is controlled by nucleoside transporters. Nucleoside transporters are divided into 2 classes. The equilibrative nucleoside transporters (ENTs) are Na+ -independent and are VXEGLYLGHGLQWR W\SHVEDVHGRQWKHLUVHQVLWLYLWLHVWR > QLWUREHQ]\O WKLRO@ ȕ ' ribofuranosylpurine (NBMPR). The concentrative nucleoside transporters (CNTs) are Na+ -dependent and are subdivided into 3 types based on the substrate selectivity. In this study, the nucleoside transporters in vascular cells were characterized and their changes in diabetes and hypertension were investigated. )XQFWLRQDOVWXGLHVVKRZHGWKDW RIWKH>3 H]adenosine transport in human brain vascular endothelial cells (HBECs) was Na+ -dependent. In the Na+ -independent component, 80% was inhibited by NBMPR (10 nM, which inhibits ENT1 but not ENT2). However, the [3 H]adenosine transport in vascular smooth muscle cells (HBSMCs) was totally Na+ -independent and 95% of it was inhibited by NBMPR (10 nM). RT-PCR revealed the presence of mRNA of ENT1, ENT2 and CNT2 in HBECs, but no CNT2 was found in HBSMCs. The unique presence of CNT2 in endothelial cells was confirmed by the observation that mRNA of ENT1, ENT2, and CNT2 were present in rat basilar arteries but no CNT2 was detected after removal of endothelia. The mRNA expression of CNT2, but not ENT1 and ENT2, was higher in basilar arteries of spontaneously hypertensive rats and streptozotocin-induced diabetic rats compared with those in normal rats. Such increase in CNT2 in hypertension and diabetes may affect the availability of adenosine in the vicinity of adenosine receptors of endothelial cells and, thus, alter vascular functions.-
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/JVRen_HK
dc.relation.ispartofJournal of Vascular Research-
dc.rightsJournal of Vascular Research. Copyright © S Karger AG.en_HK
dc.titleDiabetes- and hypertension-induced upregulation of concentrative nucleoside transporter-2 in endothelial cellsen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1018-1172&volume=46 &issue=Supp 1&spage=178&epage=&date=2009&atitle=Diabetes-+and+hypertension-induced+upregulation+of+concentrative+nucleoside+transporter-2+in+endothelial+cellsen_HK
dc.identifier.emailLeung, GPH: leung_pak_heng@hotmail.comen_HK
dc.identifier.emailHo, YW: eywho@graduate.hku.hken_HK
dc.identifier.authorityLeung, GPH=rp00234en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1159/000222318-
dc.identifier.hkuros157523en_HK
dc.identifier.volume46-
dc.identifier.issuesuppl. 1-
dc.identifier.spage178, abstract no. P14-4-
dc.identifier.epage178, abstract no. P14-4-

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