File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Characterization of a novel tumor suppressor gene UPK1A in esophageal squanous cell carcinoma

TitleCharacterization of a novel tumor suppressor gene UPK1A in esophageal squanous cell carcinoma
Authors
Issue Date2009
PublisherAmerican Association for Cancer Research
Citation
The 100th Annual Meeting of the American Association for Cancer Research, Denver, CO, 18-22 April 2009. In Cancer Research, 2009, v. 69, Abstract no. 4444 How to Cite?
AbstractBackground: Esophageal Squamous Cell Carcinoma (ESCC) has been ranked as the sixth leading cause of cancer death over the world. Like other types of cancer, genetic variation in multiple cancer-related genes is one of the main causes of ESCC. Hence, to have a better understanding of ESCC, it is important to identify these key genes and recognize their roles. Recently, our group performed an Affemetrix cDNA Microarray comparing differentially expressed genes between ESCC tumors and their adjacent nontumorous tissues. Uroplakin 1a (UPK1A) was found to be down-regulated. In this study, the role UPK1A gene in ESCC was characterized. Methods and Results: By reverse transcription-polymerase chain reaction (RT-PCR), 43/50 (86%) primary ESCCs and 8/9 (89%) ESCC cell lines were found no expression of UPK1A which was correlated with promoter hypermethylation. In addition, functional studies such as colony formation in soft agar, cell proliferation assay, foci formation and tumor formation in nude mice showed that UPK1A could suppress the tumorigenicity of ESCC cells in vitro and in vivo. Wound healing assay and cell adhesion assay also suggested that UPK1A inhibit cell mobility and increase cell adhesion ability respectively. Conclusion: Our study indicated that UPK1A has an important suppressive ability in the development and progression which maybe a novel tumor-suppressor gene in ESCC.
Persistent Identifierhttp://hdl.handle.net/10722/62723
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorKong, KLen_HK
dc.contributor.authorFu, Len_HK
dc.contributor.authorKwong, DLWen_HK
dc.contributor.authorGuan, XYen_HK
dc.date.accessioned2010-07-13T04:07:47Z-
dc.date.available2010-07-13T04:07:47Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 100th Annual Meeting of the American Association for Cancer Research, Denver, CO, 18-22 April 2009. In Cancer Research, 2009, v. 69, Abstract no. 4444-
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/62723-
dc.description.abstractBackground: Esophageal Squamous Cell Carcinoma (ESCC) has been ranked as the sixth leading cause of cancer death over the world. Like other types of cancer, genetic variation in multiple cancer-related genes is one of the main causes of ESCC. Hence, to have a better understanding of ESCC, it is important to identify these key genes and recognize their roles. Recently, our group performed an Affemetrix cDNA Microarray comparing differentially expressed genes between ESCC tumors and their adjacent nontumorous tissues. Uroplakin 1a (UPK1A) was found to be down-regulated. In this study, the role UPK1A gene in ESCC was characterized. Methods and Results: By reverse transcription-polymerase chain reaction (RT-PCR), 43/50 (86%) primary ESCCs and 8/9 (89%) ESCC cell lines were found no expression of UPK1A which was correlated with promoter hypermethylation. In addition, functional studies such as colony formation in soft agar, cell proliferation assay, foci formation and tumor formation in nude mice showed that UPK1A could suppress the tumorigenicity of ESCC cells in vitro and in vivo. Wound healing assay and cell adhesion assay also suggested that UPK1A inhibit cell mobility and increase cell adhesion ability respectively. Conclusion: Our study indicated that UPK1A has an important suppressive ability in the development and progression which maybe a novel tumor-suppressor gene in ESCC.-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Research-
dc.titleCharacterization of a novel tumor suppressor gene UPK1A in esophageal squanous cell carcinomaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailKong, KL: karlok@hkusua.hku.hken_HK
dc.identifier.emailFu, L: gracefu@graduate.hku.hken_HK
dc.identifier.emailKwong, DLW: dlwkwong@hkucc.hku.hken_HK
dc.identifier.emailGuan, XY: xyguan@hkucc.hku.hken_HK
dc.identifier.authorityKwong, DLW=rp00414en_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.identifier.hkuros156547en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats