File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: FGFR2-positive fibroblasts provide a suitable microenvironment for tumor development and progression in esophageal carcinoma

TitleFGFR2-positive fibroblasts provide a suitable microenvironment for tumor development and progression in esophageal carcinoma
Authors
Issue Date2009
PublisherAmerican Association for Cancer Research
Citation
The 100th Annual Meeting of the American Association for Cancer Research, Denver, CO, 18-22 April 2009. In Cancer Research, 2009, v. 69, Abstract no. 3496 How to Cite?
AbstractBackground & Aims: Tumor fibroblasts (TFs) have been suggested to play an essential role in the complex process of tumor-stroma interactions and tumorigenesis. The aim of the present study was to investigate the specific role of TF in the esophageal cancer microenvironment. Methods: An Affymetrix expressionmicroarray was used to compare gene expression profiles between six pairs of TFs and normal fibroblasts (NFs) from esophageal squamous cell carcinoma (ESCC). Differentially expressed genes were identified and a subset was validated by semi-quantitative reverse transcription-PCR (RT-PCR). Results: About 43% (126/292) of known deregulated genes in TFs were associated with cell proliferation, extracellular matrix remodeling, and immune response. Up-regulation of FGFR2, which showed the most significant change, was detected in all six tested TFs as compared to their paired NFs. A further study found that FGFR2-positive fibroblasts were only observed in tumor tissues and not in tumor-surrounding stromal tissues, suggesting that FGFR2 could be used as a TF-specific marker in ESCC. Moreover, conditioned medium (CM) from TFs was found to be able to promote ESCC tumor cell growth, migration and invasion in vitro. Conclusions: Our study provides new candidate genes for the esophageal cancer microenvironment. Based on our results, we hypothesize thatFGFR2(+)-TFs might provide cancer cells with a suitable microenvironment via secretion of proteins that could promote cancer development and progression through stimulation of cancer cell proliferation, induction of angiogenesis, inhibition of cell adhesion, enhancement of cell mobility, and promotion of the epithelial-mesenchymal transition (EMT).
Persistent Identifierhttp://hdl.handle.net/10722/62705
ISSN
2015 Impact Factor: 8.556
2015 SCImago Journal Rankings: 5.372

 

DC FieldValueLanguage
dc.contributor.authorGuan, XYen_HK
dc.contributor.authorZhang, Cen_HK
dc.contributor.authorFu, Len_HK
dc.contributor.authorFu, Jen_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorLi, Yen_HK
dc.contributor.authorYang, Hen_HK
dc.contributor.authorLiu, Hen_HK
dc.date.accessioned2010-07-13T04:07:24Z-
dc.date.available2010-07-13T04:07:24Z-
dc.date.issued2009en_HK
dc.identifier.citationThe 100th Annual Meeting of the American Association for Cancer Research, Denver, CO, 18-22 April 2009. In Cancer Research, 2009, v. 69, Abstract no. 3496-
dc.identifier.issn0008-5472-
dc.identifier.urihttp://hdl.handle.net/10722/62705-
dc.description.abstractBackground & Aims: Tumor fibroblasts (TFs) have been suggested to play an essential role in the complex process of tumor-stroma interactions and tumorigenesis. The aim of the present study was to investigate the specific role of TF in the esophageal cancer microenvironment. Methods: An Affymetrix expressionmicroarray was used to compare gene expression profiles between six pairs of TFs and normal fibroblasts (NFs) from esophageal squamous cell carcinoma (ESCC). Differentially expressed genes were identified and a subset was validated by semi-quantitative reverse transcription-PCR (RT-PCR). Results: About 43% (126/292) of known deregulated genes in TFs were associated with cell proliferation, extracellular matrix remodeling, and immune response. Up-regulation of FGFR2, which showed the most significant change, was detected in all six tested TFs as compared to their paired NFs. A further study found that FGFR2-positive fibroblasts were only observed in tumor tissues and not in tumor-surrounding stromal tissues, suggesting that FGFR2 could be used as a TF-specific marker in ESCC. Moreover, conditioned medium (CM) from TFs was found to be able to promote ESCC tumor cell growth, migration and invasion in vitro. Conclusions: Our study provides new candidate genes for the esophageal cancer microenvironment. Based on our results, we hypothesize thatFGFR2(+)-TFs might provide cancer cells with a suitable microenvironment via secretion of proteins that could promote cancer development and progression through stimulation of cancer cell proliferation, induction of angiogenesis, inhibition of cell adhesion, enhancement of cell mobility, and promotion of the epithelial-mesenchymal transition (EMT).-
dc.languageengen_HK
dc.publisherAmerican Association for Cancer Research-
dc.relation.ispartofCancer Research-
dc.titleFGFR2-positive fibroblasts provide a suitable microenvironment for tumor development and progression in esophageal carcinomaen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailGuan, XY: xyguan@hkucc.hku.hken_HK
dc.identifier.emailFu, L: gracefu@graduate.hku.hken_HK
dc.identifier.emailHu, L: lhuc@hkusua.hku.hken_HK
dc.identifier.authorityGuan, XY=rp00454en_HK
dc.identifier.hkuros156543en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats