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Conference Paper: The -301T/C of Sclerostin(SOST) Modulates Bone Mineral Density by Wnt and Estrogen Signaling Pathways

TitleThe -301T/C of Sclerostin(SOST) Modulates Bone Mineral Density by Wnt and Estrogen Signaling Pathways
Authors
Issue Date2008
PublisherAmerican Society for Bone and Mineral Research
Citation
30th Annual Meeting of American Society for Bone and Mineral Research, Montreal, Canada, 12-16 September 2008, Presentation no. 1270 How to Cite?
AbstractOsteoporosis is a complex disease influenced by both genetic and environmental factors. Accumulating evidence shows that genes which cause monogenic diseases also contribute to similar complex disease in the general population. We sought to determine whether the allelic variation in seven monogenic bone disease genes (CLCN7, TCIRGI, SOST, CA2, CSTK, TGFB1 and SLC26A2) contributes to osteoporosis / bone mineral density (BMD) variation in the normal Chinese population. We conducted a gene-wide and tag SNP-based association study in 1, 243 case-control Chinese subjects. The cases were subjects with low BMD (Z-scores ≤ -1.28, equivalent to the lowest 10 percent of the population) at either the L1-4 lumbar spine or femoral neck. Control subjects had high BMD (Z-score ≥ +1.0) at the corresponding sites. Twenty-two tag SNPs (tSNPs) from seven monogenic bone disease genes were selected based on the CHB panel of the Phase II HapMap Project (r2 > 0.8 and minor allele frequency >0.2), and were genotyped using the high-throughput sequenom platform. Allelic and haplotype association analyses were conducted by Haploview and binary logistic regression analyses. Gene-gene interactions were investigated using multifactor dimensionality reduction method. AliBaba 2.1 was applied to predict the putative transcription factor binding sites at the promoter polymorphism. The promoter SNP rs1230399 (-301T/C) of SOST showed significant genotypic and allelic associations with BMD at all skeletal sites measured (P = 0.04-0.001), including the lumbar spine, femoral neck, trochanter and total hip. The haplotype CC consisting of rs1230399 and rs865429 showed consistent associations with high BMD at femoral neck and spine (P = 0.005, 0.002, respectively). Importantly, this association has been replicated in the Caucasian population. Functional analysis showed that the rs1230399 was located at the core consensus recognition site of two important transcription factors C/EBPα and FOXA1 which were involved in the Wnt and estrogen signaling pathway. T→C mutation abolishes the binding of both C/EBPα and FOXA1 to SOST. In addition, significant gene-gene interactions were identified for SOST/CLCN7 and TGFB1. In conclusion, the C-allele of -301T>C variant of SOST was associated with high BMD. The variant may mediate BMD by Wnt and estrogen signaling pathways.
Persistent Identifierhttp://hdl.handle.net/10722/62419

 

DC FieldValueLanguage
dc.contributor.authorHuang, Qen_HK
dc.contributor.authorLi, GHYen_HK
dc.contributor.authorKung, AWCen_HK
dc.date.accessioned2010-07-13T04:00:50Z-
dc.date.available2010-07-13T04:00:50Z-
dc.date.issued2008en_HK
dc.identifier.citation30th Annual Meeting of American Society for Bone and Mineral Research, Montreal, Canada, 12-16 September 2008, Presentation no. 1270-
dc.identifier.urihttp://hdl.handle.net/10722/62419-
dc.description.abstractOsteoporosis is a complex disease influenced by both genetic and environmental factors. Accumulating evidence shows that genes which cause monogenic diseases also contribute to similar complex disease in the general population. We sought to determine whether the allelic variation in seven monogenic bone disease genes (CLCN7, TCIRGI, SOST, CA2, CSTK, TGFB1 and SLC26A2) contributes to osteoporosis / bone mineral density (BMD) variation in the normal Chinese population. We conducted a gene-wide and tag SNP-based association study in 1, 243 case-control Chinese subjects. The cases were subjects with low BMD (Z-scores ≤ -1.28, equivalent to the lowest 10 percent of the population) at either the L1-4 lumbar spine or femoral neck. Control subjects had high BMD (Z-score ≥ +1.0) at the corresponding sites. Twenty-two tag SNPs (tSNPs) from seven monogenic bone disease genes were selected based on the CHB panel of the Phase II HapMap Project (r2 > 0.8 and minor allele frequency >0.2), and were genotyped using the high-throughput sequenom platform. Allelic and haplotype association analyses were conducted by Haploview and binary logistic regression analyses. Gene-gene interactions were investigated using multifactor dimensionality reduction method. AliBaba 2.1 was applied to predict the putative transcription factor binding sites at the promoter polymorphism. The promoter SNP rs1230399 (-301T/C) of SOST showed significant genotypic and allelic associations with BMD at all skeletal sites measured (P = 0.04-0.001), including the lumbar spine, femoral neck, trochanter and total hip. The haplotype CC consisting of rs1230399 and rs865429 showed consistent associations with high BMD at femoral neck and spine (P = 0.005, 0.002, respectively). Importantly, this association has been replicated in the Caucasian population. Functional analysis showed that the rs1230399 was located at the core consensus recognition site of two important transcription factors C/EBPα and FOXA1 which were involved in the Wnt and estrogen signaling pathway. T→C mutation abolishes the binding of both C/EBPα and FOXA1 to SOST. In addition, significant gene-gene interactions were identified for SOST/CLCN7 and TGFB1. In conclusion, the C-allele of -301T>C variant of SOST was associated with high BMD. The variant may mediate BMD by Wnt and estrogen signaling pathways.-
dc.languageengen_HK
dc.publisherAmerican Society for Bone and Mineral Research-
dc.relation.ispartofAnnual Meeting of American Society for Bone and Mineral Research-
dc.titleThe -301T/C of Sclerostin(SOST) Modulates Bone Mineral Density by Wnt and Estrogen Signaling Pathwaysen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailHuang, Q: qyhuang@hotmail.comen_HK
dc.identifier.emailKung, AWC: awckung@hku.hken_HK
dc.identifier.authorityHuang, Q=rp00521en_HK
dc.identifier.authorityKung, AWC=rp00368en_HK
dc.identifier.hkuros152659en_HK

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