File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Conference Paper: Immobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds

TitleImmobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffolds
Authors
Issue Date2010
Citation
Ceramic Transactions, 2010, v. 218, p. 43-51 How to Cite?
AbstractIn bone tissue engineering, an effective and sustained delivery of growth factors such as bFGF, VEGF and BMP-2 at the target site is of significant importance. Heparin, a sulfated polysaccharide, is known to have the binding affinity with a number of growth factors and thus capable of blocking their degradation and prolonging their release time. In this study, the surface modification of three dimensional Ca-P/PHBV scaffolds produced via selective laser sintering was achieved through physical entrapment of gelatin by using a miscible mixture of a solvent and a non-solvent for PHBV, with gelatin being dissolved in the mixture. The amount of gelatin entrapped on the surface of scaffold struts was determined quantitatively by bicinchoninic acid (BCA) kit assay. After surface modification, a hydrophilicity/hydrophobicity balance was obtained for the surface of scaffold struts. Heparin was subsequently surface immobilized through covalent conjugation onto gelatin-modified scaffolds. The amount of heparin conjugated to the scaffold surface was determined using the toluidine blue method. Ca-P/PHBV scaffolds with entrapped gelatin and immobilized heparin should have enhanced binding of growth factors and hence promote cell proliferation and bone tissue regeneration.
DescriptionProceedings of the 8th Pacific Rim Conference on Ceramics and Glass Technology
Persistent Identifierhttp://hdl.handle.net/10722/62269
ISSN
2015 SCImago Journal Rankings: 0.150
References

 

DC FieldValueLanguage
dc.contributor.authorDuan, Ben_HK
dc.contributor.authorWang, Men_HK
dc.date.accessioned2010-07-13T03:57:34Z-
dc.date.available2010-07-13T03:57:34Z-
dc.date.issued2010en_HK
dc.identifier.citationCeramic Transactions, 2010, v. 218, p. 43-51en_HK
dc.identifier.issn1042-1122en_HK
dc.identifier.urihttp://hdl.handle.net/10722/62269-
dc.descriptionProceedings of the 8th Pacific Rim Conference on Ceramics and Glass Technologyen_HK
dc.description.abstractIn bone tissue engineering, an effective and sustained delivery of growth factors such as bFGF, VEGF and BMP-2 at the target site is of significant importance. Heparin, a sulfated polysaccharide, is known to have the binding affinity with a number of growth factors and thus capable of blocking their degradation and prolonging their release time. In this study, the surface modification of three dimensional Ca-P/PHBV scaffolds produced via selective laser sintering was achieved through physical entrapment of gelatin by using a miscible mixture of a solvent and a non-solvent for PHBV, with gelatin being dissolved in the mixture. The amount of gelatin entrapped on the surface of scaffold struts was determined quantitatively by bicinchoninic acid (BCA) kit assay. After surface modification, a hydrophilicity/hydrophobicity balance was obtained for the surface of scaffold struts. Heparin was subsequently surface immobilized through covalent conjugation onto gelatin-modified scaffolds. The amount of heparin conjugated to the scaffold surface was determined using the toluidine blue method. Ca-P/PHBV scaffolds with entrapped gelatin and immobilized heparin should have enhanced binding of growth factors and hence promote cell proliferation and bone tissue regeneration.en_HK
dc.languageengen_HK
dc.relation.ispartofCeramic Transactionsen_HK
dc.titleImmobilization of heparin on gelatin modified three-dimensional osteoconductive Ca-P/PHBV nanocomposite scaffoldsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailWang, M:memwang@hku.hken_HK
dc.identifier.authorityWang, M=rp00185en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-77956026942en_HK
dc.identifier.hkuros157761en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956026942&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume218en_HK
dc.identifier.spage43en_HK
dc.identifier.epage51en_HK
dc.identifier.scopusauthoridDuan, B=7005042335en_HK
dc.identifier.scopusauthoridWang, M=15749714100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats