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Article: Drug carrier systems based on collagen-alginate composite structures for improving the performance of GDNF-secreting HEK293 cells

TitleDrug carrier systems based on collagen-alginate composite structures for improving the performance of GDNF-secreting HEK293 cells
Authors
KeywordsAlginate
Collagen
GDNF
HEK293 cells
Microencapsulation
Microsphere
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterials
Citation
Biomaterials, 2009, v. 30 n. 6, p. 1214-1221 How to Cite?
AbstractGlial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor. Development of drug delivery technologies facilitating controlled release of GDNF is critical to applying GDNF in treating neurodegenerative diseases. We previously developed 3D collagen microspheres and demonstrated enhanced GDNF secretion after encapsulation of HEK293 cells, which were transduced to overexpress GDNF in these microspheres. However, the entrapped HEK293 cells were able to migrate out of the collagen microspheres, making it undesirable for clinical applications. In this report, we investigate two new carrier designs, namely collagen-alginate composite gel and collagen microspheres embedded in alginate gel in preventing cell leakage, maintaining cell growth and controlling GDNF secretion in the HEK293 cells. We demonstrated that inclusion of alginate gel in both designs is efficient in preventing cell leakage to the surrounding yet permitting the GDNF secretion, although the cellular growth rate is reduced in an alginate concentration dependent manner. Differential patterns of GDNF secretion in the two designs were demonstrated. The collagen-alginate composite gel maintains a more or less constant GDNF secretion over time while the collagen microspheres embedded in alginate gel continue to increase the secretion level of GDNF over time. This study contributes towards the development of cell-based GDNF delivery devices for the future therapeutics of neurodegenerative diseases. © 2008 Elsevier Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/62256
ISSN
2015 Impact Factor: 8.387
2015 SCImago Journal Rankings: 3.565
ISI Accession Number ID
Funding AgencyGrant Number
University Research Committee10208239
Strategic Research Theme on Nanobiotechnology, the University of Hong Kong
Funding Information:

This work was supported by funds from University Research Committee, HKU (Grant No. 10208239) and Strategic Research Theme on Nanobiotechnology, the University of Hong Kong. The authors thank Ms. Carrie Wong for assistance in the GDNF analysis.

References

 

DC FieldValueLanguage
dc.contributor.authorLee, Men_HK
dc.contributor.authorLo, ACen_HK
dc.contributor.authorCheung, PTen_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorChan, BPen_HK
dc.date.accessioned2010-07-13T03:57:18Z-
dc.date.available2010-07-13T03:57:18Z-
dc.date.issued2009en_HK
dc.identifier.citationBiomaterials, 2009, v. 30 n. 6, p. 1214-1221en_HK
dc.identifier.issn0142-9612en_HK
dc.identifier.urihttp://hdl.handle.net/10722/62256-
dc.description.abstractGlial cell line-derived neurotrophic factor (GDNF) is a potent neurotrophic factor. Development of drug delivery technologies facilitating controlled release of GDNF is critical to applying GDNF in treating neurodegenerative diseases. We previously developed 3D collagen microspheres and demonstrated enhanced GDNF secretion after encapsulation of HEK293 cells, which were transduced to overexpress GDNF in these microspheres. However, the entrapped HEK293 cells were able to migrate out of the collagen microspheres, making it undesirable for clinical applications. In this report, we investigate two new carrier designs, namely collagen-alginate composite gel and collagen microspheres embedded in alginate gel in preventing cell leakage, maintaining cell growth and controlling GDNF secretion in the HEK293 cells. We demonstrated that inclusion of alginate gel in both designs is efficient in preventing cell leakage to the surrounding yet permitting the GDNF secretion, although the cellular growth rate is reduced in an alginate concentration dependent manner. Differential patterns of GDNF secretion in the two designs were demonstrated. The collagen-alginate composite gel maintains a more or less constant GDNF secretion over time while the collagen microspheres embedded in alginate gel continue to increase the secretion level of GDNF over time. This study contributes towards the development of cell-based GDNF delivery devices for the future therapeutics of neurodegenerative diseases. © 2008 Elsevier Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/biomaterialsen_HK
dc.relation.ispartofBiomaterialsen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in <Journal title>. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in PUBLICATION, [VOL#, ISSUE#, (DATE)] DOI#-
dc.subjectAlginateen_HK
dc.subjectCollagenen_HK
dc.subjectGDNFen_HK
dc.subjectHEK293 cellsen_HK
dc.subjectMicroencapsulationen_HK
dc.subjectMicrosphereen_HK
dc.subject.meshAlginates - pharmacology-
dc.subject.meshCollagen - pharmacology-
dc.subject.meshDrug Carriers-
dc.subject.meshGlial Cell Line-Derived Neurotrophic Factor - secretion-
dc.subject.meshMicrospheres-
dc.titleDrug carrier systems based on collagen-alginate composite structures for improving the performance of GDNF-secreting HEK293 cellsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0142-9612&volume=30&issue=6&spage=1214&epage=1221&date=2009&atitle=Drug+carrier+systems+based+on+collagen-alginate+composite+structures+for+improving+the+performance+of+GDNF-secreting+HEK293+cells-
dc.identifier.emailLo, AC: amylo@hkucc.hku.hken_HK
dc.identifier.emailCheung, PT: ptcheung@hku.hken_HK
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.emailChan, BP: bpchan@hkucc.hku.hken_HK
dc.identifier.authorityLo, AC=rp00425en_HK
dc.identifier.authorityCheung, PT=rp00351en_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.identifier.authorityChan, BP=rp00087en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.biomaterials.2008.11.017en_HK
dc.identifier.pmid19059641-
dc.identifier.scopuseid_2-s2.0-57749189581en_HK
dc.identifier.hkuros164822en_HK
dc.identifier.hkuros164805-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57749189581&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume30en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1214en_HK
dc.identifier.epage1221en_HK
dc.identifier.eissn1878-5905-
dc.identifier.isiWOS:000262750300025-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLee, M=22980015700en_HK
dc.identifier.scopusauthoridLo, AC=7102780640en_HK
dc.identifier.scopusauthoridCheung, PT=7202595465en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridChan, BP=7201530390en_HK

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