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Conference Paper: Magnetic resonance imaging of migrating neuronal precursors in normal and hypoxic-ischemic neonatal rat brains by intraventricular MPIO labeling

TitleMagnetic resonance imaging of migrating neuronal precursors in normal and hypoxic-ischemic neonatal rat brains by intraventricular MPIO labeling
Authors
Issue Date2008
PublisherIEEE. The Journal's web site is located at http://www.ieeexplore.ieee.org/xpl/conhome.jsp?punumber=1000269
Citation
Proceedings Of The 30Th Annual International Conference Of The Ieee Engineering In Medicine And Biology Society, Embs'08 - Personalized Healthcare Through Technology, 2008, p. 363-366 How to Cite?
AbstractIn this study, 10-day-old normal rats (n=6) and hypoxic-ischemic (H-I) neonatal rats (n=6) were injected with the micronsized iron oxide particles (MPIOs) into the anterior lateral ventricle. 2D and 3D high-spatial resolution MRI were performed with a 7T animal scanner 1 day before the MPIOs injection and hour 3, day 3, day 7 and day 14 after the MPIOs injection. Intraperitoneal injections of 5-bromo-2'-deoxyuridine (BrdU) were used to label newly produced cells, and were given thrice daily for 2 days before sacrifice. Immunohistochemistry and Prussian blue staining indicated that iron particles were inside the nestin+ and BrdU+ neural progenitor cells (NPCs), glial-fibrillary-acidic-protein-positive (GFAP+) astrocytes-like progenitor cells, and neuronal-nuclei-positive (NeuN+) mature neurons. Here we demonstrate that, in normal neonatal rat brain, the migrating pathway of the endogenous NPCs with MPIO is mainly along the rostral migratory stream to the olfactory bulb. In H-I neonatal rat brain, the migration of endogenous NPCs with MPIO is mainly towards the ischemic regions. Therefore, in vivo magnetic cell labeling of endogenous NPCs with MPIO and subsequently non-invasive, serial MRI monitoring should open up a new approach to probe into the mechanism of cell migration in the developmental brain under physiological and pathologic conditions. © 2008 IEEE.
DescriptionProceedings of the IEEE Engineering in Medicine and Biology Society Conference, 2008, p. 363-366
Persistent Identifierhttp://hdl.handle.net/10722/62061
ISSN
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Jen_HK
dc.contributor.authorLiu, Jen_HK
dc.contributor.authorNiu, Gen_HK
dc.contributor.authorLiu, Yen_HK
dc.contributor.authorWu, EXen_HK
dc.date.accessioned2010-07-13T03:53:06Z-
dc.date.available2010-07-13T03:53:06Z-
dc.date.issued2008en_HK
dc.identifier.citationProceedings Of The 30Th Annual International Conference Of The Ieee Engineering In Medicine And Biology Society, Embs'08 - Personalized Healthcare Through Technology, 2008, p. 363-366en_HK
dc.identifier.issn1557-170Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/62061-
dc.descriptionProceedings of the IEEE Engineering in Medicine and Biology Society Conference, 2008, p. 363-366en_HK
dc.description.abstractIn this study, 10-day-old normal rats (n=6) and hypoxic-ischemic (H-I) neonatal rats (n=6) were injected with the micronsized iron oxide particles (MPIOs) into the anterior lateral ventricle. 2D and 3D high-spatial resolution MRI were performed with a 7T animal scanner 1 day before the MPIOs injection and hour 3, day 3, day 7 and day 14 after the MPIOs injection. Intraperitoneal injections of 5-bromo-2'-deoxyuridine (BrdU) were used to label newly produced cells, and were given thrice daily for 2 days before sacrifice. Immunohistochemistry and Prussian blue staining indicated that iron particles were inside the nestin+ and BrdU+ neural progenitor cells (NPCs), glial-fibrillary-acidic-protein-positive (GFAP+) astrocytes-like progenitor cells, and neuronal-nuclei-positive (NeuN+) mature neurons. Here we demonstrate that, in normal neonatal rat brain, the migrating pathway of the endogenous NPCs with MPIO is mainly along the rostral migratory stream to the olfactory bulb. In H-I neonatal rat brain, the migration of endogenous NPCs with MPIO is mainly towards the ischemic regions. Therefore, in vivo magnetic cell labeling of endogenous NPCs with MPIO and subsequently non-invasive, serial MRI monitoring should open up a new approach to probe into the mechanism of cell migration in the developmental brain under physiological and pathologic conditions. © 2008 IEEE.en_HK
dc.languageengen_HK
dc.publisherIEEE. The Journal's web site is located at http://www.ieeexplore.ieee.org/xpl/conhome.jsp?punumber=1000269-
dc.relation.ispartofProceedings of the 30th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS'08 - "Personalized Healthcare through Technology"en_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsIEEE Engineering in Medicine and Biology Society. Conference Proceedings. Copyright © IEEE.-
dc.rights©2008 IEEE. Personal use of this material is permitted. However, permission to reprint/republish this material for advertising or promotional purposes or for creating new collective works for resale or redistribution to servers or lists, or to reuse any copyrighted component of this work in other works must be obtained from the IEEE.-
dc.subject.meshBrain - pathology-
dc.subject.meshCerebral Ventricles - pathology-
dc.subject.meshFerric Compounds - diagnostic use-
dc.subject.meshHypoxia-Ischemia, Brain - pathology-
dc.subject.meshImage Enhancement - methods-
dc.titleMagnetic resonance imaging of migrating neuronal precursors in normal and hypoxic-ischemic neonatal rat brains by intraventricular MPIO labelingen_HK
dc.typeConference_Paperen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1557-170X&volume=&spage=363&epage=366&date=2008&atitle=Magnetic+resonance+imaging+of+migrating+neuronal+precursors+in+normal+and+hypoxic-ischemic+neonatal+rat+brains+by+intraventricular+MPIO+labeling-
dc.identifier.emailWu, EX:ewu1@hkucc.hku.hken_HK
dc.identifier.authorityWu, EX=rp00193en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1109/IEMBS.2008.4649165-
dc.identifier.pmid19162668en_HK
dc.identifier.scopuseid_2-s2.0-61849126270en_HK
dc.identifier.hkuros161995en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61849126270&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.spage363en_HK
dc.identifier.epage366en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridYang, J=10041733800en_HK
dc.identifier.scopusauthoridLiu, J=35201658300en_HK
dc.identifier.scopusauthoridNiu, G=35273273000en_HK
dc.identifier.scopusauthoridLiu, Y=26642953800en_HK
dc.identifier.scopusauthoridWu, EX=7202128034en_HK

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