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Conference Paper: Green tea polyphenols (GTPS) reduced fibrogenesis in non-alcoholic fatty liver disease in rats

TitleGreen tea polyphenols (GTPS) reduced fibrogenesis in non-alcoholic fatty liver disease in rats
Authors
Issue Date2008
PublisherThe American Association for the Study of Liver Diseases
John Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
The 59th Annual Meeting of the American Association for the Study of Liver Diseases (AASLD), San Francisco, CA., 31 October-4 November 2008. In Hepatology, 2008, v. 48 n. S1, p. 841A, abstract no.1195 How to Cite?
AbstractBackground: Attention has been focused on the use of supple-mentary phytochemicals in the treatment of non-alcoholic fattyliver disease (NAFLD). Objective: We investigated whetherEGCG could modulate expression of profibrogenic markers inNAFLD. Experimental Design: We fed an unsaturated fat diet(30% fish oil) to female Sprague-Dawley rats (180-200g), con-sumed ad libitum for 8 weeks (NAFLD; n = 8). Control animals(CF; n = 8) were fed an isocaloric diet with commercial ratchow. To evaluate the effect of EGCG on NAFLD, rats in theNAFLD group was injected with EGCG intraperitoneally[50mg/kg] three times per week. The CF group received thevehicle. At killing, blood and liver samples were collected forserum alanine aminotransferase (ALT), histology and molecularanalysis. Each histological sample was evaluated for fatty liver(graded from 0-4+), necrosis (number of necrotic foci(no./mm2) and inflammation (cells per mm2). The amount ofcollagen formation was estimated using Sirius Red staining.Reverse transcription polymerase chain reaction (RT-PCR) wascarried out for TGF-β1, procollagen-I (Pro-col-I), tissue inhibitorsof metalloproteinases (TIMP-1, TIMP-2) matrix metalloproteinase(MMP-2). Zymography determined the enzyme activity of MMP-2. Electrophoretic mobility shift assay was performed fornuclear factor-kappa B (NF-kB) activity. Results: NAFLD rats hada significantly higher serum ALT level, fatty liver, necrosis,inflammation and amount of collagen formation (Sirius Redstaining) when compared with the CF rats (p< 0.05). ThemRNA levels of the profibrotic factors, TGF-β1, Pro-col-I, TIMP-1, TIMP-2 and MMP-2 were elevated in the NAFLD group (p<0.05). The MMP-2 enzyme and NF-kB activity were alsoincreased in the NAFLD rats (p<0.01). Treatment with EGCGsignificantly reduced the severity of pathological changeswhich include fatty change, necrosis and inflammation, fibrosis(Sirius Red) and down-regulated expression of TGF-β1, Pro-col-I, TIMP-1, TIMP-2 and MMP-2. Treatment with EGCG also sig-nificantly decreased the MMP-2 enzyme and NF-kB activity.Conclusion: EGCG decreased the degree of hepatic fibrosisand profibrogenic markers in rats fed with fish and dextrose.We proposed that the therapeutic effect of GTPs in NAFLD maybe mediated through down-regulation of profibrogenic factorsand its use can be considered as adjuvant therapy in fiborgenicliver disease.
Persistent Identifierhttp://hdl.handle.net/10722/61434
ISSN
2015 Impact Factor: 11.711
2015 SCImago Journal Rankings: 4.752

 

DC FieldValueLanguage
dc.contributor.authorTipoe, GLen_HK
dc.contributor.authorHo, MCTen_HK
dc.contributor.authorLiong, ECen_HK
dc.contributor.authorLeung, TMen_HK
dc.contributor.authorLau, TYHen_HK
dc.contributor.authorFung, MLen_HK
dc.contributor.authorNanji, AAen_HK
dc.date.accessioned2010-07-13T03:39:36Z-
dc.date.available2010-07-13T03:39:36Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 59th Annual Meeting of the American Association for the Study of Liver Diseases (AASLD), San Francisco, CA., 31 October-4 November 2008. In Hepatology, 2008, v. 48 n. S1, p. 841A, abstract no.1195en_HK
dc.identifier.issn0270-9139-
dc.identifier.urihttp://hdl.handle.net/10722/61434-
dc.description.abstractBackground: Attention has been focused on the use of supple-mentary phytochemicals in the treatment of non-alcoholic fattyliver disease (NAFLD). Objective: We investigated whetherEGCG could modulate expression of profibrogenic markers inNAFLD. Experimental Design: We fed an unsaturated fat diet(30% fish oil) to female Sprague-Dawley rats (180-200g), con-sumed ad libitum for 8 weeks (NAFLD; n = 8). Control animals(CF; n = 8) were fed an isocaloric diet with commercial ratchow. To evaluate the effect of EGCG on NAFLD, rats in theNAFLD group was injected with EGCG intraperitoneally[50mg/kg] three times per week. The CF group received thevehicle. At killing, blood and liver samples were collected forserum alanine aminotransferase (ALT), histology and molecularanalysis. Each histological sample was evaluated for fatty liver(graded from 0-4+), necrosis (number of necrotic foci(no./mm2) and inflammation (cells per mm2). The amount ofcollagen formation was estimated using Sirius Red staining.Reverse transcription polymerase chain reaction (RT-PCR) wascarried out for TGF-β1, procollagen-I (Pro-col-I), tissue inhibitorsof metalloproteinases (TIMP-1, TIMP-2) matrix metalloproteinase(MMP-2). Zymography determined the enzyme activity of MMP-2. Electrophoretic mobility shift assay was performed fornuclear factor-kappa B (NF-kB) activity. Results: NAFLD rats hada significantly higher serum ALT level, fatty liver, necrosis,inflammation and amount of collagen formation (Sirius Redstaining) when compared with the CF rats (p< 0.05). ThemRNA levels of the profibrotic factors, TGF-β1, Pro-col-I, TIMP-1, TIMP-2 and MMP-2 were elevated in the NAFLD group (p<0.05). The MMP-2 enzyme and NF-kB activity were alsoincreased in the NAFLD rats (p<0.01). Treatment with EGCGsignificantly reduced the severity of pathological changeswhich include fatty change, necrosis and inflammation, fibrosis(Sirius Red) and down-regulated expression of TGF-β1, Pro-col-I, TIMP-1, TIMP-2 and MMP-2. Treatment with EGCG also sig-nificantly decreased the MMP-2 enzyme and NF-kB activity.Conclusion: EGCG decreased the degree of hepatic fibrosisand profibrogenic markers in rats fed with fish and dextrose.We proposed that the therapeutic effect of GTPs in NAFLD maybe mediated through down-regulation of profibrogenic factorsand its use can be considered as adjuvant therapy in fiborgenicliver disease.-
dc.languageengen_HK
dc.publisherThe American Association for the Study of Liver Diseases-
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/-
dc.relation.ispartofHepatology-
dc.rightsHepatology. Copyright © John Wiley & Sons, Inc.-
dc.titleGreen tea polyphenols (GTPS) reduced fibrogenesis in non-alcoholic fatty liver disease in ratsen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailTipoe, GL: tgeorge@hkucc.hku.hken_HK
dc.identifier.emailHo, MCT: emike99@netvigator.comen_HK
dc.identifier.emailLiong, EC: eclionga@HKUCC.hku.hken_HK
dc.identifier.emailLeung, TM: leungtm@hkucc.hku.hken_HK
dc.identifier.emailFung, ML: fungml@hkucc.hku.hken_HK
dc.identifier.authorityTipoe, GL=rp00371en_HK
dc.identifier.authorityFung, ML=rp00433en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/hep.22645-
dc.identifier.hkuros155492en_HK
dc.identifier.hkuros160575-
dc.identifier.volume48-
dc.identifier.issuesuppl. 1-
dc.identifier.spage841A, abstract no.1195-
dc.identifier.epage841A, abstract no.1195-
dc.publisher.placeUnited States-

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