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Conference Paper: Id1 overexpression induces tetraploidization and multiple abnormal mitotic phenotypes by modulating aurora A.

TitleId1 overexpression induces tetraploidization and multiple abnormal mitotic phenotypes by modulating aurora A.
Authors
Issue Date2008
PublisherThe American Society for Cell Biology.
Citation
The 48th Annual Meeting of the American Society for Cell Biology (ASCB 2008), San Francisco, CA., 13-17 December 2008. In Regular Anstracts, 2008, p. 634, abstract no. 2187/B656 How to Cite?
AbstractThe basic helix-loop-helix transcription factor, Id1, was shown to induce tetraploidy in telomerase-immortalized nasopharyngeal epithelial cells in this study. Using both transient and stable Id1-expressing cell models, multiple mitotic aberrations were detected; including centrosome amplification, binucleation, spindle defects and microtubule perturbation. Many of these abnormal phenotypes have previously been reported in cells overexpressing Aurora A. Further experiments showed that Id1 could stabilize Aurora A, while knocking down Aurora A expression in Id1-expressing cells could rescue some of the mitotic defects. The mechanisms by which Aurora A could be modulated by Id1 were explored. DNA amplification of the Aurora A locus was not involved. Id1 could only weakly activate the transcriptional activity of the Aurora A promoter. We found that Id1 overexpression could affect Aurora A degradation, leading to its stabilization. Aurora A is normally degraded from mitosis exit by the APC/C Cdh1-mediated proteasomal proteolysis pathway. Our results revealed that Id1 and Cdh1 are binding partners. The association of Id1 and Cdh1 was found to be dependent on the canonical destruction box (D box) motif of Id1; the increased binding of which may compete with the interaction between cdh1 and Aurora A, leading to stabilization of Aurora A in Id1-overexpressing cells.
DescriptionSession - Cancer III (2168 – 2195): abstract no. 2187/B656
Persistent Identifierhttp://hdl.handle.net/10722/61428

 

DC FieldValueLanguage
dc.contributor.authorMan, CWYen_HK
dc.contributor.authorDoxsey, Sen_HK
dc.contributor.authorTsao, GSWen_HK
dc.date.accessioned2010-07-13T03:39:28Z-
dc.date.available2010-07-13T03:39:28Z-
dc.date.issued2008en_HK
dc.identifier.citationThe 48th Annual Meeting of the American Society for Cell Biology (ASCB 2008), San Francisco, CA., 13-17 December 2008. In Regular Anstracts, 2008, p. 634, abstract no. 2187/B656-
dc.identifier.urihttp://hdl.handle.net/10722/61428-
dc.descriptionSession - Cancer III (2168 – 2195): abstract no. 2187/B656en_HK
dc.description.abstractThe basic helix-loop-helix transcription factor, Id1, was shown to induce tetraploidy in telomerase-immortalized nasopharyngeal epithelial cells in this study. Using both transient and stable Id1-expressing cell models, multiple mitotic aberrations were detected; including centrosome amplification, binucleation, spindle defects and microtubule perturbation. Many of these abnormal phenotypes have previously been reported in cells overexpressing Aurora A. Further experiments showed that Id1 could stabilize Aurora A, while knocking down Aurora A expression in Id1-expressing cells could rescue some of the mitotic defects. The mechanisms by which Aurora A could be modulated by Id1 were explored. DNA amplification of the Aurora A locus was not involved. Id1 could only weakly activate the transcriptional activity of the Aurora A promoter. We found that Id1 overexpression could affect Aurora A degradation, leading to its stabilization. Aurora A is normally degraded from mitosis exit by the APC/C Cdh1-mediated proteasomal proteolysis pathway. Our results revealed that Id1 and Cdh1 are binding partners. The association of Id1 and Cdh1 was found to be dependent on the canonical destruction box (D box) motif of Id1; the increased binding of which may compete with the interaction between cdh1 and Aurora A, leading to stabilization of Aurora A in Id1-overexpressing cells.-
dc.languageengen_HK
dc.publisherThe American Society for Cell Biology.-
dc.relation.ispartof48th ASCB Annual Meeting 2008: Regular Abstracts-
dc.titleId1 overexpression induces tetraploidization and multiple abnormal mitotic phenotypes by modulating aurora A.en_HK
dc.typeConference_Paperen_HK
dc.identifier.emailMan, CWY: cornman@hkucc.hku.hken_HK
dc.identifier.emailTsao, GSW: gswtsao@hku.hken_HK
dc.identifier.authorityTsao, GSW=rp00399en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros165265en_HK
dc.identifier.spage634-
dc.identifier.epage634-
dc.publisher.placeUnited States-

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