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Conference Paper: Self-assembled nanomaterial, chondroitinase ABC and CNTF combination treatment provides a permissive environment for optic-tract regeneration in hamster

TitleSelf-assembled nanomaterial, chondroitinase ABC and CNTF combination treatment provides a permissive environment for optic-tract regeneration in hamster
Authors
Issue Date2008
PublisherSociety for Neuroscience
Citation
Neuroscience 2008, Washington, DC, 15-19 November 2008, Program#/Poster#: 725.28/D2 How to Cite?
AbstractA tissue gap caused by deep transections of the optic tract (OT) in the midbrain can completely block the reinnervation of the superior colliculus (SC) by the retina. We find that a self-assembling peptide (SAP) nanofiber scaffold used in combination with ciliary neurotrophic factor (CNTF) and Chondroitinase ABC (Chon. ABC) can synergistically facilitate the reconstruction of a tissue substrate that supports regeneration across the tissue disruption. Brain wounds were inflicted in anesthetized adult Syrian hamsters. The scalp and skull were opened surgically and the OT at the brachium of the SC was completely severed with a deep knife wound, extending 1-2 mm below the surface from the midline to a point beyond the lateral margin of SC. Three groups of young adult hamsters (8 wk) were prepared. In group one (SAP/CNTF), following the transection of the brachium of SC the animals were treated with 20μl of 1% SAP RAD16-I injected into the site of injury. In group two (Chon.ABC/CNTF), 20 μl of 2.5units/ml Chon.ABC was injected into the lesion site. In group three (SAP/Chon.ABC/CNFT), both SAP and Chon.ABC were injected. These experimental animals received 5 intravitreal injections of 1μg CNTF in 1μl PBS once every five days. Four days prior to sacrifice CTB-FITC was injected intravitreally to anterogradely label the regenerating axons. Histological results revealed that there was no reinnervation of SC 4 weeks after surgery in the SAP/CNTF and Chon.ABC/CNTF groups of animals. However, CTB-FITC was found in the SC of the animals treated with SAP/Chon.ABC/CNTF implying that the regenerated axons had reinnervated the target. In behavioral studies the adult hamsters, after 4 wk post surgery, showed a functional return of vision only in the third group, treated with SAP/Chon.ABC/CNTF. Thus, the SAP/Chon.ABC/CNTF combination treatment is shown to offer an effective new means of creating a permissive environment for axonal growth, allowing regrowth of axons into the SC.
Persistent Identifierhttp://hdl.handle.net/10722/61415

 

DC FieldValueLanguage
dc.contributor.authorSchneider, GEen_HK
dc.contributor.authorLiang, Yen_HK
dc.contributor.authorTay, DKCen_HK
dc.contributor.authorSo, KFen_HK
dc.contributor.authorEllis-Behnke, RGen_HK
dc.date.accessioned2010-07-13T03:39:13Z-
dc.date.available2010-07-13T03:39:13Z-
dc.date.issued2008en_HK
dc.identifier.citationNeuroscience 2008, Washington, DC, 15-19 November 2008, Program#/Poster#: 725.28/D2en_HK
dc.identifier.urihttp://hdl.handle.net/10722/61415-
dc.description.abstractA tissue gap caused by deep transections of the optic tract (OT) in the midbrain can completely block the reinnervation of the superior colliculus (SC) by the retina. We find that a self-assembling peptide (SAP) nanofiber scaffold used in combination with ciliary neurotrophic factor (CNTF) and Chondroitinase ABC (Chon. ABC) can synergistically facilitate the reconstruction of a tissue substrate that supports regeneration across the tissue disruption. Brain wounds were inflicted in anesthetized adult Syrian hamsters. The scalp and skull were opened surgically and the OT at the brachium of the SC was completely severed with a deep knife wound, extending 1-2 mm below the surface from the midline to a point beyond the lateral margin of SC. Three groups of young adult hamsters (8 wk) were prepared. In group one (SAP/CNTF), following the transection of the brachium of SC the animals were treated with 20μl of 1% SAP RAD16-I injected into the site of injury. In group two (Chon.ABC/CNTF), 20 μl of 2.5units/ml Chon.ABC was injected into the lesion site. In group three (SAP/Chon.ABC/CNFT), both SAP and Chon.ABC were injected. These experimental animals received 5 intravitreal injections of 1μg CNTF in 1μl PBS once every five days. Four days prior to sacrifice CTB-FITC was injected intravitreally to anterogradely label the regenerating axons. Histological results revealed that there was no reinnervation of SC 4 weeks after surgery in the SAP/CNTF and Chon.ABC/CNTF groups of animals. However, CTB-FITC was found in the SC of the animals treated with SAP/Chon.ABC/CNTF implying that the regenerated axons had reinnervated the target. In behavioral studies the adult hamsters, after 4 wk post surgery, showed a functional return of vision only in the third group, treated with SAP/Chon.ABC/CNTF. Thus, the SAP/Chon.ABC/CNTF combination treatment is shown to offer an effective new means of creating a permissive environment for axonal growth, allowing regrowth of axons into the SC.-
dc.languageengen_HK
dc.publisherSociety for Neuroscience-
dc.relation.ispartofSociety for Neuroscience Annual Meeting-
dc.titleSelf-assembled nanomaterial, chondroitinase ABC and CNTF combination treatment provides a permissive environment for optic-tract regeneration in hamsteren_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLiang, Y: yxliang99@yahoo.com.cnen_HK
dc.identifier.emailTay, DKC: dkctay@hkucc.hku.hken_HK
dc.identifier.emailTay, DKC: dkctay@hkucc.hku.hken_HK
dc.identifier.emailSo, KF: hrmaskf@hkucc.hku.hken_HK
dc.identifier.emailEllis-Behnke, RG: rutledg@mit.eduen_HK
dc.identifier.authorityLiang, Y=rp00510en_HK
dc.identifier.authorityTay, DKC=rp00336en_HK
dc.identifier.authorityTay, DKC=rp00336en_HK
dc.identifier.authoritySo, KF=rp00329en_HK
dc.identifier.authorityEllis-Behnke, RG=rp00252en_HK
dc.identifier.hkuros154557en_HK

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