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Article: The distribution, release kinetics, and biocompatibility of Triamcinolone injected and dispersed in silicone oil

TitleThe distribution, release kinetics, and biocompatibility of Triamcinolone injected and dispersed in silicone oil
Authors
Issue Date2009
PublisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.org
Citation
Investigative Ophthalmology And Visual Science, 2009, v. 50 n. 5, p. 2337-2343 How to Cite?
AbstractPURPOSE. Triamcinolone acetonide (TA) has been proposed as an adjuvant to pars plana vitrectomy with silicone oil for the surgical treatment of proliferative vitreoretinopathy and proliferative diabetic retinopathy. However, to date no data about the distribution and pharmacokinetics of lipophilic TA injected into silicone oil have been reported. METHODS. An artificial vitreous space chamber was filled with silicone oil. TA was either injected or dispersed into silicone oil. TA release using a continuous flow model was measured spectrophotometrically. To determine the antiproliferative or cytotoxic effect of the released TA, monolayer cultures of retinal pigment epithelial cells (ARPE19) and retinal ganglion cells (RGC5) were used. Bromodeoxyuridine incorporation, MTT assay, and scanning electron microscopy were performed. RESULTS. Injected TA sank slowly through the silicone oil and started to sediment below the silicone oil bubble shortly after injection. After the simulated intravitreal injection, no TA could be retrieved from the silicone oil bubble. In contrast, when a suspension of silicone oil and TA was prepared before injection, stable noncytotoxic amounts of TA (25 μg/mL) could be retrieved for up to 90 days. After mere injection (without previous suspension in silicone oil), the sedimented TA crystals showed a pronounced cytotoxic effect. CONCLUSIONS. Intravitreally injected TA does not mix with silicone oil. TA crystals that sediment at the lower border of a silicone oil bubble may be harmful to retinal cells. A suspension of TA in silicone oil may exhibit safer extended release over several days. © Association for Research in Vision and Ophthalmology.
Persistent Identifierhttp://hdl.handle.net/10722/60896
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 2.008
ISI Accession Number ID
Funding AgencyGrant Number
European Community
Marie Curie Research GrantWLG5-CT-2001-60034
Funding Information:

Supported by the European Community and by Marie Curie Research Grant WLG5-CT-2001-60034.

References

 

DC FieldValueLanguage
dc.contributor.authorSpitzer, MSen_HK
dc.contributor.authorKaczmarek, RTen_HK
dc.contributor.authorYoeruek, Een_HK
dc.contributor.authorPetermeier, Ken_HK
dc.contributor.authorWong, Den_HK
dc.contributor.authorHeimann, Hen_HK
dc.contributor.authorJaissle, GBen_HK
dc.contributor.authorBartzSchmidt, KUen_HK
dc.contributor.authorSzurman, Pen_HK
dc.date.accessioned2010-05-31T04:21:22Z-
dc.date.available2010-05-31T04:21:22Z-
dc.date.issued2009en_HK
dc.identifier.citationInvestigative Ophthalmology And Visual Science, 2009, v. 50 n. 5, p. 2337-2343en_HK
dc.identifier.issn0146-0404en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60896-
dc.description.abstractPURPOSE. Triamcinolone acetonide (TA) has been proposed as an adjuvant to pars plana vitrectomy with silicone oil for the surgical treatment of proliferative vitreoretinopathy and proliferative diabetic retinopathy. However, to date no data about the distribution and pharmacokinetics of lipophilic TA injected into silicone oil have been reported. METHODS. An artificial vitreous space chamber was filled with silicone oil. TA was either injected or dispersed into silicone oil. TA release using a continuous flow model was measured spectrophotometrically. To determine the antiproliferative or cytotoxic effect of the released TA, monolayer cultures of retinal pigment epithelial cells (ARPE19) and retinal ganglion cells (RGC5) were used. Bromodeoxyuridine incorporation, MTT assay, and scanning electron microscopy were performed. RESULTS. Injected TA sank slowly through the silicone oil and started to sediment below the silicone oil bubble shortly after injection. After the simulated intravitreal injection, no TA could be retrieved from the silicone oil bubble. In contrast, when a suspension of silicone oil and TA was prepared before injection, stable noncytotoxic amounts of TA (25 μg/mL) could be retrieved for up to 90 days. After mere injection (without previous suspension in silicone oil), the sedimented TA crystals showed a pronounced cytotoxic effect. CONCLUSIONS. Intravitreally injected TA does not mix with silicone oil. TA crystals that sediment at the lower border of a silicone oil bubble may be harmful to retinal cells. A suspension of TA in silicone oil may exhibit safer extended release over several days. © Association for Research in Vision and Ophthalmology.en_HK
dc.languageengen_HK
dc.publisherAssociation for Research in Vision and Ophthalmology. The Journal's web site is located at http://www.iovs.orgen_HK
dc.relation.ispartofInvestigative Ophthalmology and Visual Scienceen_HK
dc.subject.meshEye - metabolism-
dc.subject.meshGlucocorticoids - pharmacokinetics - toxicity-
dc.subject.meshModels, Biological-
dc.subject.meshSilicone Oils - metabolism-
dc.subject.meshTriamcinolone Acetonide - pharmacokinetics - toxicity-
dc.titleThe distribution, release kinetics, and biocompatibility of Triamcinolone injected and dispersed in silicone oilen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0146-0404&volume=50&issue=5&spage=2337&epage=2343&date=2008&atitle=The+distribution,+release+kinetics,+and+biocompatibility+of+triamcinolone+injected+and+dispersed+in+silicone+oil-
dc.identifier.emailWong, D: shdwong@hku.hken_HK
dc.identifier.authorityWong, D=rp00516en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1167/iovs.08-2471en_HK
dc.identifier.pmid19098319-
dc.identifier.scopuseid_2-s2.0-65549107419en_HK
dc.identifier.hkuros154793en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65549107419&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume50en_HK
dc.identifier.issue5en_HK
dc.identifier.spage2337en_HK
dc.identifier.epage2343en_HK
dc.identifier.isiWOS:000265451000049-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridSpitzer, MS=14016728300en_HK
dc.identifier.scopusauthoridKaczmarek, RT=7006704264en_HK
dc.identifier.scopusauthoridYoeruek, E=23029503600en_HK
dc.identifier.scopusauthoridPetermeier, K=23567218900en_HK
dc.identifier.scopusauthoridWong, D=7401536078en_HK
dc.identifier.scopusauthoridHeimann, H=26535875100en_HK
dc.identifier.scopusauthoridJaissle, GB=6603048877en_HK
dc.identifier.scopusauthoridBartzSchmidt, KU=7005611257en_HK
dc.identifier.scopusauthoridSzurman, P=6701352874en_HK

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