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Article: Curcumin inhibits cell proliferation of MDA-MB-231 and BT-483 breast cancer cells mediated by down-regulation of NFκB, cyclinD and MMP-1 transcription

TitleCurcumin inhibits cell proliferation of MDA-MB-231 and BT-483 breast cancer cells mediated by down-regulation of NFκB, cyclinD and MMP-1 transcription
Authors
KeywordsCurcuma longa
Curcumin
CyclinD1/CDK4
MMPs
NFκB
Issue Date2009
PublisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomed
Citation
Phytomedicine, 2009, v. 16 n. 10, p. 916-922 How to Cite?
AbstractCurcumin, an active constituent of turmeric, has been shown to possess inhibitory effect of cell proliferation and induction of apoptosis towards a board range of tumors. Cell inhibition activities of curcumin are behaved differently in various cell types. To investigate the mechanism basis for the cell inhibition of curcumin on breast cancer cell lines, we examine curcumin effect on NFκB, cell cycle regulatory proteins and matrix metalloproteinases (MMPs) in two breast cancer cell lines (MDA-MB-231 and BT-483). Cell proliferation was performed by water soluble tetrazolium WST-1 assay. The effect of curcumin's on the activity of matrix metalloproteinase-1, 3, 9 were analyzed by RT-PCR. Cell cycle regulatory protein including cyclin D1, CDK4 and p21 were examined by immunochemistry. The expressions of NFκB in breast cancer cells treated with curcumin were studied by immunochemistry and western blot. The results from WST-1 cell proliferation assay showed that curcumin exhibited the anti-proliferation effect on MDA-MB-231 and BT-483 cells in a time- and dose-dependent manner. In response to the treatment, while, the expression of cyclin D1 had declined in MDA-MB-231 and the expression of CDK4 in BT-483 had declined. MMP1 mRNA expression in BT-483 and MDA-MB-231 had significantly decreased in curcumin treatment group compared with control group. Our finding extrapolates the antitumor activity of curcumin in mediating the breast cancer cell proliferative rate and invasion by down-regulating the NFκB inducing genes. © 2009 Elsevier GmbH. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/60791
ISSN
2015 Impact Factor: 2.937
2015 SCImago Journal Rankings: 1.013
ISI Accession Number ID
Funding AgencyGrant Number
Seed Funding Programme for Basic Research
University of Hong Kong200611159207
Funding Information:

This research was supported in part by grant from Seed Funding Programme for Basic Research, the University of Hong Kong (No. 200611159207).

References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorLoo, WTYen_HK
dc.contributor.authorSze, SCWen_HK
dc.contributor.authorTong, Yen_HK
dc.date.accessioned2010-05-31T04:18:39Z-
dc.date.available2010-05-31T04:18:39Z-
dc.date.issued2009en_HK
dc.identifier.citationPhytomedicine, 2009, v. 16 n. 10, p. 916-922en_HK
dc.identifier.issn0944-7113en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60791-
dc.description.abstractCurcumin, an active constituent of turmeric, has been shown to possess inhibitory effect of cell proliferation and induction of apoptosis towards a board range of tumors. Cell inhibition activities of curcumin are behaved differently in various cell types. To investigate the mechanism basis for the cell inhibition of curcumin on breast cancer cell lines, we examine curcumin effect on NFκB, cell cycle regulatory proteins and matrix metalloproteinases (MMPs) in two breast cancer cell lines (MDA-MB-231 and BT-483). Cell proliferation was performed by water soluble tetrazolium WST-1 assay. The effect of curcumin's on the activity of matrix metalloproteinase-1, 3, 9 were analyzed by RT-PCR. Cell cycle regulatory protein including cyclin D1, CDK4 and p21 were examined by immunochemistry. The expressions of NFκB in breast cancer cells treated with curcumin were studied by immunochemistry and western blot. The results from WST-1 cell proliferation assay showed that curcumin exhibited the anti-proliferation effect on MDA-MB-231 and BT-483 cells in a time- and dose-dependent manner. In response to the treatment, while, the expression of cyclin D1 had declined in MDA-MB-231 and the expression of CDK4 in BT-483 had declined. MMP1 mRNA expression in BT-483 and MDA-MB-231 had significantly decreased in curcumin treatment group compared with control group. Our finding extrapolates the antitumor activity of curcumin in mediating the breast cancer cell proliferative rate and invasion by down-regulating the NFκB inducing genes. © 2009 Elsevier GmbH. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherUrban und Fischer Verlag. The Journal's web site is located at http://www.elsevier.com/locate/phytomeden_HK
dc.relation.ispartofPhytomedicineen_HK
dc.rightsNOTICE: this is the author’s version of a work that was accepted for publication in Phytomedicine. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Phytomedicine, 2009, v. 16 n. 10, p. 916-922. DOI: 10.1016/j.phymed.2009.04.008-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectCurcuma longaen_HK
dc.subjectCurcuminen_HK
dc.subjectCyclinD1/CDK4en_HK
dc.subjectMMPsen_HK
dc.subjectNFκBen_HK
dc.subject.meshCell Proliferation - drug effects-
dc.subject.meshCurcumin - pharmacology - therapeutic use-
dc.subject.meshCyclin D - metabolism-
dc.subject.meshMatrix Metalloproteinase 1 - metabolism-
dc.subject.meshNF-kappa B - metabolism-
dc.titleCurcumin inhibits cell proliferation of MDA-MB-231 and BT-483 breast cancer cells mediated by down-regulation of NFκB, cyclinD and MMP-1 transcriptionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0944-7113&volume=&spage=&epage=&date=2009&atitle=Curcumin+inhibits+cell+proliferation+of+MDA-MB-231+and+BT-483+breast+cancer+cells+through+down-regulation+of+NFκB,+cyclinD/CDK4+and+MMP-1+transcriptionen_HK
dc.identifier.emailSze, SCW: stephens@hku.hken_HK
dc.identifier.emailTong, Y: tongyao@hku.hken_HK
dc.identifier.authoritySze, SCW=rp00514en_HK
dc.identifier.authorityTong, Y=rp00509en_HK
dc.description.naturepostprint-
dc.identifier.doi10.1016/j.phymed.2009.04.008en_HK
dc.identifier.pmid19524420-
dc.identifier.scopuseid_2-s2.0-69849091814en_HK
dc.identifier.hkuros162844en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-69849091814&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue10en_HK
dc.identifier.spage916en_HK
dc.identifier.epage922en_HK
dc.identifier.isiWOS:000270773600003-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLiu, Q=36063708000en_HK
dc.identifier.scopusauthoridLoo, WTY=7003567474en_HK
dc.identifier.scopusauthoridSze, SCW=23482617000en_HK
dc.identifier.scopusauthoridTong, Y=9045384000en_HK
dc.identifier.citeulike6905567-

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