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Article: Cloning, tissue distribution, and functional characterization of chicken glucagon receptor

TitleCloning, tissue distribution, and functional characterization of chicken glucagon receptor
Authors
KeywordsChicken
Glucagon
Glucagon receptor
Glucagon receptor variant
Issue Date2008
PublisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.org
Citation
Poultry Science, 2008, v. 87 n. 12, p. 2678-2688 How to Cite?
AbstractGlucagon has been reported to play an important role in hepatic glucose metabolism of vertebrates including birds. However, the molecular mechanism of its actions in birds remains largely unknown. In present study, the full-length cDNA of chicken glucagon receptor (GCGR) was first cloned from brain tissue using reverse transcription-PCR. This putative chicken GCGR (named cGCGR-s) is 496 amino acids (AA) long and shares high AA sequence identity with that of human (70%), rat (69%), mouse (69%), and Xenopus (64%), and a comparatively lower identity with goldfish (53%). In addition, a full-length cDNA encoding a novel glucagon receptor variant (named cGCGR-v1) of 554 AA was identified in this study. Sequence analysis revealed that this receptor variant arises from the retention of intron 4 (174 bp) and thus causes a 58-AA insertion at the large N-terminal extracellular domain. Using the pGL3-CRE-luciferase reporter system, we demonstrated that human glucagon could potently activate chicken GCGR-s and GCGR-v1 expressed in Chinese hamster ovary cells, confirming that both cGCGR-s and cGCGR-v1 are functional and able to couple to the intracellular cyclic adenosine monophosphate-protein kinase A signaling pathway. Using a reverse transcription-PCR assay, we further examined the expression of glucagon receptor in adult chicken tissues, including different regions of the brain. Glucagon receptor was shown to be highly expressed in liver and moderately or weakly expressed in other tissues examined. In the central nervous system, the greatest expression was consistently detected in the hypothalamus. Taken together, our data not only suggest that glucagon receptor plays a critical role in mediating the actions of glucagon in liver, but also imply that glucagon may have important roles in nonhepatic tissues, such as in the hypothalamus of brain in chickens. ©2008 Poultry Science Association Inc.
Persistent Identifierhttp://hdl.handle.net/10722/60729
ISSN
2015 Impact Factor: 1.685
2015 SCImago Journal Rankings: 1.162
ISI Accession Number ID
Funding AgencyGrant Number
Council of the Hong Kong GovernmentHKU7345/03M
National Natural Science Foundation of China30700452
Funding Information:

This work was supported by grants from the Research Grant Council of the Hong Kong Government to Frederick C. Leung (HKU7345/03M) and the National Natural Science Foundation of China to Li Juan (30700452).

References

 

DC FieldValueLanguage
dc.contributor.authorWang, Jen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorLi, Xen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorLeung, FCen_HK
dc.date.accessioned2010-05-31T04:17:17Z-
dc.date.available2010-05-31T04:17:17Z-
dc.date.issued2008en_HK
dc.identifier.citationPoultry Science, 2008, v. 87 n. 12, p. 2678-2688en_HK
dc.identifier.issn0032-5791en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60729-
dc.description.abstractGlucagon has been reported to play an important role in hepatic glucose metabolism of vertebrates including birds. However, the molecular mechanism of its actions in birds remains largely unknown. In present study, the full-length cDNA of chicken glucagon receptor (GCGR) was first cloned from brain tissue using reverse transcription-PCR. This putative chicken GCGR (named cGCGR-s) is 496 amino acids (AA) long and shares high AA sequence identity with that of human (70%), rat (69%), mouse (69%), and Xenopus (64%), and a comparatively lower identity with goldfish (53%). In addition, a full-length cDNA encoding a novel glucagon receptor variant (named cGCGR-v1) of 554 AA was identified in this study. Sequence analysis revealed that this receptor variant arises from the retention of intron 4 (174 bp) and thus causes a 58-AA insertion at the large N-terminal extracellular domain. Using the pGL3-CRE-luciferase reporter system, we demonstrated that human glucagon could potently activate chicken GCGR-s and GCGR-v1 expressed in Chinese hamster ovary cells, confirming that both cGCGR-s and cGCGR-v1 are functional and able to couple to the intracellular cyclic adenosine monophosphate-protein kinase A signaling pathway. Using a reverse transcription-PCR assay, we further examined the expression of glucagon receptor in adult chicken tissues, including different regions of the brain. Glucagon receptor was shown to be highly expressed in liver and moderately or weakly expressed in other tissues examined. In the central nervous system, the greatest expression was consistently detected in the hypothalamus. Taken together, our data not only suggest that glucagon receptor plays a critical role in mediating the actions of glucagon in liver, but also imply that glucagon may have important roles in nonhepatic tissues, such as in the hypothalamus of brain in chickens. ©2008 Poultry Science Association Inc.en_HK
dc.languageengen_HK
dc.publisherPoultry Science Association Inc. The Journal's web site is located at http://ps.fass.orgen_HK
dc.relation.ispartofPoultry Scienceen_HK
dc.subjectChickenen_HK
dc.subjectGlucagonen_HK
dc.subjectGlucagon receptoren_HK
dc.subjectGlucagon receptor varianten_HK
dc.subject.meshAmino Acid Sequence-
dc.subject.meshAnimals-
dc.subject.meshChickens - genetics - metabolism-
dc.subject.meshCloning, Molecular-
dc.subject.meshReceptors, Glucagon - chemistry - genetics - metabolism-
dc.titleCloning, tissue distribution, and functional characterization of chicken glucagon receptoren_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0032-5791&volume=87&issue=12&spage=2678&epage=2688&date=2008&atitle=Cloning,+tissue+distribution,+and+functional+characterization+of+chicken+glucagon+receptoren_HK
dc.identifier.emailWang, Y: cdwyj@yahoo.comen_HK
dc.identifier.emailLeung, FC: fcleung@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00801en_HK
dc.identifier.authorityLeung, FC=rp00731en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3382/ps.2008-00260en_HK
dc.identifier.pmid19038826-
dc.identifier.scopuseid_2-s2.0-57049096035en_HK
dc.identifier.hkuros166178en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57049096035&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume87en_HK
dc.identifier.issue12en_HK
dc.identifier.spage2678en_HK
dc.identifier.epage2688en_HK
dc.identifier.isiWOS:000261601300031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, J=7701326167en_HK
dc.identifier.scopusauthoridWang, Y=36062525200en_HK
dc.identifier.scopusauthoridLi, X=9532654300en_HK
dc.identifier.scopusauthoridLi, J=36077227000en_HK
dc.identifier.scopusauthoridLeung, FC=7103078633en_HK

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