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Article: A Brassica juncea chitinase with two-chitin binding domains show anti-microbial properties against phytopathogens and Gram-negative bacteria

TitleA Brassica juncea chitinase with two-chitin binding domains show anti-microbial properties against phytopathogens and Gram-negative bacteria
Authors
KeywordsBacterial agglutination
Chitin-binding domain
Indian mustard
Lectin
Phytopathogens
Pichia-expressed proteins
Transplastomic tobacco
Issue Date2008
PublisherLandes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/psb
Citation
Plant Signaling And Behavior, 2008, v. 3 n. 12, p. 1103-1105 How to Cite?
AbstractIn our recent paper in the Journal of Experimental Botany, we demonstrated that Brassica juncea BjCHI1 shows anti-fungal properties against phytopathogens, Colletotrichum truncatum, C. acutatum, Botrytis cinerea and Ascochyta rabiei. Furthermore, BjCHI1 which is an unusual plant chitinase with two (almost identical) chitin-binding domains, agglutinates Gram-negative bacteria, adversely affecting their growth. In contrast, BjCHI1 derivatives lacking one or both domains do not show agglutination activity, suggesting that both chitin-binding domains are essential for agglutination. Observations that agglutination could be relieved by addition of galactose, glucose or lactose, imply that BjCHI1 interacts with the carbohydrate components of the Gram-negative bacterial cell wall. We propose here, a model for BjCHI1-mediated agglutination between Gram-negative bacteria, through interaction of their adjacent cell walls mediated by the two chitin-binding domains of BjCHI1. BjCHI1 is a plant chitinase which has evolved towards acquiring an enhanced role in plant defense against fungi and Gram-negative bacteria. Hence, it is a promising candidate for applications against phytopathogens in plant genetic engineering via nuclear or plastid transformation. ©2008 Landes Bioscience.
Persistent Identifierhttp://hdl.handle.net/10722/60720
ISSN
2015 SCImago Journal Rankings: 0.633
References

 

DC FieldValueLanguage
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-05-31T04:17:07Z-
dc.date.available2010-05-31T04:17:07Z-
dc.date.issued2008en_HK
dc.identifier.citationPlant Signaling And Behavior, 2008, v. 3 n. 12, p. 1103-1105en_HK
dc.identifier.issn1559-2316en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60720-
dc.description.abstractIn our recent paper in the Journal of Experimental Botany, we demonstrated that Brassica juncea BjCHI1 shows anti-fungal properties against phytopathogens, Colletotrichum truncatum, C. acutatum, Botrytis cinerea and Ascochyta rabiei. Furthermore, BjCHI1 which is an unusual plant chitinase with two (almost identical) chitin-binding domains, agglutinates Gram-negative bacteria, adversely affecting their growth. In contrast, BjCHI1 derivatives lacking one or both domains do not show agglutination activity, suggesting that both chitin-binding domains are essential for agglutination. Observations that agglutination could be relieved by addition of galactose, glucose or lactose, imply that BjCHI1 interacts with the carbohydrate components of the Gram-negative bacterial cell wall. We propose here, a model for BjCHI1-mediated agglutination between Gram-negative bacteria, through interaction of their adjacent cell walls mediated by the two chitin-binding domains of BjCHI1. BjCHI1 is a plant chitinase which has evolved towards acquiring an enhanced role in plant defense against fungi and Gram-negative bacteria. Hence, it is a promising candidate for applications against phytopathogens in plant genetic engineering via nuclear or plastid transformation. ©2008 Landes Bioscience.en_HK
dc.languageengen_HK
dc.publisherLandes Bioscience. The Journal's web site is located at http://www.landesbioscience.com/journals/psben_HK
dc.relation.ispartofPlant Signaling and Behavioren_HK
dc.subjectBacterial agglutinationen_HK
dc.subjectChitin-binding domainen_HK
dc.subjectIndian mustarden_HK
dc.subjectLectinen_HK
dc.subjectPhytopathogensen_HK
dc.subjectPichia-expressed proteinsen_HK
dc.subjectTransplastomic tobaccoen_HK
dc.titleA Brassica juncea chitinase with two-chitin binding domains show anti-microbial properties against phytopathogens and Gram-negative bacteriaen_HK
dc.typeArticleen_HK
dc.identifier.emailGuan, Y: yguan@hkucc.hku.hken_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityGuan, Y=rp00397en_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.scopuseid_2-s2.0-57749111535en_HK
dc.identifier.hkuros154146en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57749111535&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue12en_HK
dc.identifier.spage1103en_HK
dc.identifier.epage1105en_HK
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridGuan, Y=7202924055en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

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