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Article: Vertical partitioning and expression of primary metabolic genes in a thermophilic microbial mat

TitleVertical partitioning and expression of primary metabolic genes in a thermophilic microbial mat
Authors
KeywordsAnoxygenic photosynthesis
Nitrogenase
RUBISCO
Thermophiles
Issue Date2009
PublisherSpringer Japan. The Journal's web site is located at http://link.springer-ny.com/link/service/journals/00792/index.htm
Citation
Extremophiles, 2009, v. 13 n. 3, p. 533-540 How to Cite?
AbstractA thermophilic microbial mat with a relatively simple morphological composition was used to study the expression of key metabolic genes between mat layers. Mats comprised Roseiflexus castenholzii, Synechococcus sp., a Sphingomonas-like proteobacterial taxon and an unidentified member of the Thermotogae as determined by 16S rRNA phylotypes. The diversity of expressed loci for key genes involved in oxygenic photosynthesis (cbbL), anoxygenic photosynthesis (pufM) and nitrogen fixation (nifH) was assessed. The cyanobacterial surface layer supported two cbbL transcripts, with closest phylogenetic affinity to those from the cyanobacterium Synechococcus sp. and a proteobacterium Nitrobacter sp. This indicates that both photoautotrophic and chemolithoautotrophic carbon dioxide fixation may occur in this mat layer. Lower layers did not support cbbL transcripts. Anoxygenic photosynthesis was indicated by a single pufM transcript with closest affinity to that of R. castenholzii. Expression occurred in all layers beneath the cyanobacterial surface layer. Expression of a single nifH transcript with closest affinity to a proteobacterial nitrogenase occurred in samples throughout all mat layers. © 2009 Springer.
Persistent Identifierhttp://hdl.handle.net/10722/60719
ISSN
2015 Impact Factor: 2.346
2015 SCImago Journal Rankings: 0.972
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grants CouncilHKU 7573/05M
Funding Information:

The authors are grateful for financial support from the Hong Kong Research Grants Council (grant number HKU 7573/05M).

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLau, MCYen_HK
dc.contributor.authorPointing, SBen_HK
dc.date.accessioned2010-05-31T04:17:06Z-
dc.date.available2010-05-31T04:17:06Z-
dc.date.issued2009en_HK
dc.identifier.citationExtremophiles, 2009, v. 13 n. 3, p. 533-540en_HK
dc.identifier.issn1431-0651en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60719-
dc.description.abstractA thermophilic microbial mat with a relatively simple morphological composition was used to study the expression of key metabolic genes between mat layers. Mats comprised Roseiflexus castenholzii, Synechococcus sp., a Sphingomonas-like proteobacterial taxon and an unidentified member of the Thermotogae as determined by 16S rRNA phylotypes. The diversity of expressed loci for key genes involved in oxygenic photosynthesis (cbbL), anoxygenic photosynthesis (pufM) and nitrogen fixation (nifH) was assessed. The cyanobacterial surface layer supported two cbbL transcripts, with closest phylogenetic affinity to those from the cyanobacterium Synechococcus sp. and a proteobacterium Nitrobacter sp. This indicates that both photoautotrophic and chemolithoautotrophic carbon dioxide fixation may occur in this mat layer. Lower layers did not support cbbL transcripts. Anoxygenic photosynthesis was indicated by a single pufM transcript with closest affinity to that of R. castenholzii. Expression occurred in all layers beneath the cyanobacterial surface layer. Expression of a single nifH transcript with closest affinity to a proteobacterial nitrogenase occurred in samples throughout all mat layers. © 2009 Springer.en_HK
dc.languageengen_HK
dc.publisherSpringer Japan. The Journal's web site is located at http://link.springer-ny.com/link/service/journals/00792/index.htmen_HK
dc.relation.ispartofExtremophilesen_HK
dc.subjectAnoxygenic photosynthesisen_HK
dc.subjectNitrogenaseen_HK
dc.subjectRUBISCOen_HK
dc.subjectThermophilesen_HK
dc.titleVertical partitioning and expression of primary metabolic genes in a thermophilic microbial maten_HK
dc.typeArticleen_HK
dc.identifier.emailLau, MCY: maglau@princeton.eduen_HK
dc.identifier.emailPointing, SB: pointing@hkucc.hku.hken_HK
dc.identifier.authorityLau, MCY=rp00721en_HK
dc.identifier.authorityPointing, SB=rp00771en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1007/s00792-009-0240-8en_HK
dc.identifier.pmid19347567en_HK
dc.identifier.scopuseid_2-s2.0-67349183223en_HK
dc.identifier.hkuros160621en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67349183223&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume13en_HK
dc.identifier.issue3en_HK
dc.identifier.spage533en_HK
dc.identifier.epage540en_HK
dc.identifier.isiWOS:000265917900014-
dc.publisher.placeJapanen_HK
dc.relation.projectMolecular diversity and phylogeography of thermophilic communities within geothermal springs in southeast Asia and the contribution of adaptation and allopatry to diversity-
dc.identifier.scopusauthoridLau, MCY=35177794300en_HK
dc.identifier.scopusauthoridPointing, SB=6603986412en_HK
dc.identifier.citeulike4289189-

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