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- Publisher Website: 10.1007/s00792-009-0240-8
- Scopus: eid_2-s2.0-67349183223
- PMID: 19347567
- WOS: WOS:000265917900014
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Article: Vertical partitioning and expression of primary metabolic genes in a thermophilic microbial mat
Title | Vertical partitioning and expression of primary metabolic genes in a thermophilic microbial mat | ||||
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Authors | |||||
Keywords | Anoxygenic photosynthesis Nitrogenase RUBISCO Thermophiles | ||||
Issue Date | 2009 | ||||
Publisher | Springer Japan. The Journal's web site is located at http://link.springer-ny.com/link/service/journals/00792/index.htm | ||||
Citation | Extremophiles, 2009, v. 13 n. 3, p. 533-540 How to Cite? | ||||
Abstract | A thermophilic microbial mat with a relatively simple morphological composition was used to study the expression of key metabolic genes between mat layers. Mats comprised Roseiflexus castenholzii, Synechococcus sp., a Sphingomonas-like proteobacterial taxon and an unidentified member of the Thermotogae as determined by 16S rRNA phylotypes. The diversity of expressed loci for key genes involved in oxygenic photosynthesis (cbbL), anoxygenic photosynthesis (pufM) and nitrogen fixation (nifH) was assessed. The cyanobacterial surface layer supported two cbbL transcripts, with closest phylogenetic affinity to those from the cyanobacterium Synechococcus sp. and a proteobacterium Nitrobacter sp. This indicates that both photoautotrophic and chemolithoautotrophic carbon dioxide fixation may occur in this mat layer. Lower layers did not support cbbL transcripts. Anoxygenic photosynthesis was indicated by a single pufM transcript with closest affinity to that of R. castenholzii. Expression occurred in all layers beneath the cyanobacterial surface layer. Expression of a single nifH transcript with closest affinity to a proteobacterial nitrogenase occurred in samples throughout all mat layers. © 2009 Springer. | ||||
Persistent Identifier | http://hdl.handle.net/10722/60719 | ||||
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.726 | ||||
ISI Accession Number ID |
Funding Information: The authors are grateful for financial support from the Hong Kong Research Grants Council (grant number HKU 7573/05M). | ||||
References | |||||
Grants |
DC Field | Value | Language |
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dc.contributor.author | Lau, MCY | en_HK |
dc.contributor.author | Pointing, SB | en_HK |
dc.date.accessioned | 2010-05-31T04:17:06Z | - |
dc.date.available | 2010-05-31T04:17:06Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Extremophiles, 2009, v. 13 n. 3, p. 533-540 | en_HK |
dc.identifier.issn | 1431-0651 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/60719 | - |
dc.description.abstract | A thermophilic microbial mat with a relatively simple morphological composition was used to study the expression of key metabolic genes between mat layers. Mats comprised Roseiflexus castenholzii, Synechococcus sp., a Sphingomonas-like proteobacterial taxon and an unidentified member of the Thermotogae as determined by 16S rRNA phylotypes. The diversity of expressed loci for key genes involved in oxygenic photosynthesis (cbbL), anoxygenic photosynthesis (pufM) and nitrogen fixation (nifH) was assessed. The cyanobacterial surface layer supported two cbbL transcripts, with closest phylogenetic affinity to those from the cyanobacterium Synechococcus sp. and a proteobacterium Nitrobacter sp. This indicates that both photoautotrophic and chemolithoautotrophic carbon dioxide fixation may occur in this mat layer. Lower layers did not support cbbL transcripts. Anoxygenic photosynthesis was indicated by a single pufM transcript with closest affinity to that of R. castenholzii. Expression occurred in all layers beneath the cyanobacterial surface layer. Expression of a single nifH transcript with closest affinity to a proteobacterial nitrogenase occurred in samples throughout all mat layers. © 2009 Springer. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Springer Japan. The Journal's web site is located at http://link.springer-ny.com/link/service/journals/00792/index.htm | en_HK |
dc.relation.ispartof | Extremophiles | en_HK |
dc.subject | Anoxygenic photosynthesis | en_HK |
dc.subject | Nitrogenase | en_HK |
dc.subject | RUBISCO | en_HK |
dc.subject | Thermophiles | en_HK |
dc.title | Vertical partitioning and expression of primary metabolic genes in a thermophilic microbial mat | en_HK |
dc.type | Article | en_HK |
dc.identifier.email | Lau, MCY: maglau@princeton.edu | en_HK |
dc.identifier.email | Pointing, SB: pointing@hkucc.hku.hk | en_HK |
dc.identifier.authority | Lau, MCY=rp00721 | en_HK |
dc.identifier.authority | Pointing, SB=rp00771 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1007/s00792-009-0240-8 | en_HK |
dc.identifier.pmid | 19347567 | en_HK |
dc.identifier.scopus | eid_2-s2.0-67349183223 | en_HK |
dc.identifier.hkuros | 160621 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-67349183223&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 13 | en_HK |
dc.identifier.issue | 3 | en_HK |
dc.identifier.spage | 533 | en_HK |
dc.identifier.epage | 540 | en_HK |
dc.identifier.isi | WOS:000265917900014 | - |
dc.publisher.place | Japan | en_HK |
dc.relation.project | Molecular diversity and phylogeography of thermophilic communities within geothermal springs in southeast Asia and the contribution of adaptation and allopatry to diversity | - |
dc.identifier.scopusauthorid | Lau, MCY=35177794300 | en_HK |
dc.identifier.scopusauthorid | Pointing, SB=6603986412 | en_HK |
dc.identifier.citeulike | 4289189 | - |
dc.identifier.issnl | 1431-0651 | - |