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Article: Localization and expression pattern of cytoglobin in carbon tetrachloride-induced liver fibrosis

TitleLocalization and expression pattern of cytoglobin in carbon tetrachloride-induced liver fibrosis
Authors
KeywordsCarbon tetrachloride
Cytoglobin
Liver fibrosis
Procollagen I alpha 1
Issue Date2008
PublisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxlet
Citation
Toxicology Letters, 2008, v. 183 n. 1-3, p. 36-44 How to Cite?
AbstractCytoglobin (Cygb) is a recently discovered hexacoordinate globin protein with a yet undefined function. It was found to be up-regulated in toxin-induced liver fibrosis and during hypoxic conditions [Fago, A., Hundahl, C., Malte, H., Weber, R.E., 2004. Functional properties of neuroglobin and cytoglobin. Insights into the ancestral physiological roles of globins. IUBMB Life 56, 689-696; Fordel, E., Thijs, L., Martinet, W., Schrijvers, D., Moens, L., Dewilde, S., 2007. Anoxia or oxygen and glucose deprivation in SH-SY5Y cells: a step closer to the unraveling of neuroglobin and cytoglobin functions. Gene 398, 114-122; Guo, X., Philipsen, S., Tan-Un, K.C., 2007. Study of the hypoxia-dependent regulation of human CYGB gene. Biochem. Biophys. Res. Commun. 364, 145-150; Li, D., Chen, X.Q., Li, W.J., Yang, Y.H., Wang, J.Z., Yu, A.C., 2007. Cytoglobin up-regulated by hydrogen peroxide plays a protective role in oxidative stress. Neurochem. Res. 32, 1375-1380]. Cygb is expressed ubiquitously in most tissues but its subcellular localization in certain cell types (e.g. hepatocytes) is still controversial. In this study we investigated the localization of Cygb protein in mouse tissues, its expression pattern in response to carbon tetrachloride (CCl4) challenge and that during CCl4-induced liver fibrosis in order to delineate the functional property of Cygb. We found that it is expressed in fibroblasts in various organs and in the hepatic stellate cells. Cygb mRNA expression is up-regulated by more than 3.5-fold 24 h after administration of CCl4. At 48 h post-administration, the expression of procollagen I alpha 1 mRNA was increased by over 7.6-fold. The increase in collagen expression after CCl4 insult was also evident at the protein level. We found that the number of Cygb-expressing cells increased through the development of CCl4-induced liver fibrosis. It may be possible that Cygb is an early biomarker for liver fibrosis. © 2008 Elsevier Ireland Ltd. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/60718
ISSN
2015 Impact Factor: 3.522
2015 SCImago Journal Rankings: 1.267
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong Seed Funding
Funding Information:

This study is supported by University of Hong Kong Seed Funding. The funding party is not involved in the design, collection, analysis or interpretation of data.

References

 

DC FieldValueLanguage
dc.contributor.authorMan, KnMen_HK
dc.contributor.authorPhilipsen, Sen_HK
dc.contributor.authorTanUn, KCen_HK
dc.date.accessioned2010-05-31T04:17:05Z-
dc.date.available2010-05-31T04:17:05Z-
dc.date.issued2008en_HK
dc.identifier.citationToxicology Letters, 2008, v. 183 n. 1-3, p. 36-44en_HK
dc.identifier.issn0378-4274en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60718-
dc.description.abstractCytoglobin (Cygb) is a recently discovered hexacoordinate globin protein with a yet undefined function. It was found to be up-regulated in toxin-induced liver fibrosis and during hypoxic conditions [Fago, A., Hundahl, C., Malte, H., Weber, R.E., 2004. Functional properties of neuroglobin and cytoglobin. Insights into the ancestral physiological roles of globins. IUBMB Life 56, 689-696; Fordel, E., Thijs, L., Martinet, W., Schrijvers, D., Moens, L., Dewilde, S., 2007. Anoxia or oxygen and glucose deprivation in SH-SY5Y cells: a step closer to the unraveling of neuroglobin and cytoglobin functions. Gene 398, 114-122; Guo, X., Philipsen, S., Tan-Un, K.C., 2007. Study of the hypoxia-dependent regulation of human CYGB gene. Biochem. Biophys. Res. Commun. 364, 145-150; Li, D., Chen, X.Q., Li, W.J., Yang, Y.H., Wang, J.Z., Yu, A.C., 2007. Cytoglobin up-regulated by hydrogen peroxide plays a protective role in oxidative stress. Neurochem. Res. 32, 1375-1380]. Cygb is expressed ubiquitously in most tissues but its subcellular localization in certain cell types (e.g. hepatocytes) is still controversial. In this study we investigated the localization of Cygb protein in mouse tissues, its expression pattern in response to carbon tetrachloride (CCl4) challenge and that during CCl4-induced liver fibrosis in order to delineate the functional property of Cygb. We found that it is expressed in fibroblasts in various organs and in the hepatic stellate cells. Cygb mRNA expression is up-regulated by more than 3.5-fold 24 h after administration of CCl4. At 48 h post-administration, the expression of procollagen I alpha 1 mRNA was increased by over 7.6-fold. The increase in collagen expression after CCl4 insult was also evident at the protein level. We found that the number of Cygb-expressing cells increased through the development of CCl4-induced liver fibrosis. It may be possible that Cygb is an early biomarker for liver fibrosis. © 2008 Elsevier Ireland Ltd. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier Ireland Ltd. The Journal's web site is located at http://www.elsevier.com/locate/toxleten_HK
dc.relation.ispartofToxicology Lettersen_HK
dc.subjectCarbon tetrachlorideen_HK
dc.subjectCytoglobinen_HK
dc.subjectLiver fibrosisen_HK
dc.subjectProcollagen I alpha 1en_HK
dc.titleLocalization and expression pattern of cytoglobin in carbon tetrachloride-induced liver fibrosisen_HK
dc.typeArticleen_HK
dc.identifier.emailTanUn, KC: kctanun@hkucc.hku.hken_HK
dc.identifier.authorityTanUn, KC=rp00787en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.toxlet.2008.09.015en_HK
dc.identifier.pmid18940239-
dc.identifier.scopuseid_2-s2.0-56549090632en_HK
dc.identifier.hkuros162901en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-56549090632&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume183en_HK
dc.identifier.issue1-3en_HK
dc.identifier.spage36en_HK
dc.identifier.epage44en_HK
dc.identifier.isiWOS:000262053000005-
dc.publisher.placeIrelanden_HK
dc.identifier.scopusauthoridMan, KnM=25722804100en_HK
dc.identifier.scopusauthoridPhilipsen, S=7003745052en_HK
dc.identifier.scopusauthoridTanUn, KC=6602914262en_HK

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