File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs

TitleThe secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophs
Authors
Issue Date2009
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 2009, v. 150 n. 5, p. 2273-2282 How to Cite?
AbstractSecretoneurin (SN) isa 33-to34-aminoacid neuropeptide derived from secretogranin-II, a member of the chromogranin family. We previously synthesized a putative goldfish (gf) SN and demonstrated its ability to stimulate LH release in vivo. However, it was not known whether goldfish actually produced the free SN peptide or whether SN directly stimulates LH release from isolated pituitary cells. Using a combination of reverse-phase HPLC and mass spectrometry analysis, we isolated for the first time a 34-amino acid free gfSN peptide from the whole brain. Moreover, Western blot analysis indicated the existence of this peptide in goldfish pituitary. Immunocyto- chemical localization studies revealed the presence of SN immunoreactivity in prolactin cells of rostral pars distalis of the anterior pituitary. Additionally, we found that magnocellular cells of the goldfish preoptic region are highly immunoreactive for SN. These neurons send heavily labeled projections that pass through the pituitary stalk and innervate the neurointermediateand anterior lobes. In static 12-h incubation of dispersed pituitary cells, application of SN antiserum reduced LH levels, whereas 1 and 10 nM gfSN, respectively, induced 2.5-fold (P < 0.001) and 1.9-fold (P < 0.01) increments of LH release into the medium, increases similar to those elicited by 100 nM concentrations of GnRH. LikeGnRH, gfSN elevated intracellular Ca2+ in identified gonadotrophs. Whereas we do not yet know the relative contribution of neural SN or pituitary SN to LH release, we propose that SN could act as a neuroendocrine and/or paracrine factor to regulate LH release from the anterior pituitary. Copyright © 2009 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/60699
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID
Funding AgencyGrant Number
National Sciences and Engineering Research Council Canada
Hong Kong Research Grants Council (RGC)
University of Ottawa Interfaculty Research Program
Funding Information:

This work was supported by National Sciences and Engineering Research Council Canada (to V. L. T. and J.P.C.), Hong Kong Research Grants Council (RGC) (to A.O.L.W.), and the University of Ottawa Interfaculty Research Program (to V. L. T. and A. B.).

References

 

DC FieldValueLanguage
dc.contributor.authorZhao, Een_HK
dc.contributor.authorBasak, Aen_HK
dc.contributor.authorWong, AOLen_HK
dc.contributor.authorKo, Wen_HK
dc.contributor.authorChen, Aen_HK
dc.contributor.authorLopez, GCen_HK
dc.contributor.authorGrey, CLen_HK
dc.contributor.authorCanosa, LFen_HK
dc.contributor.authorSomoza, GMen_HK
dc.contributor.authorChang, JPen_HK
dc.contributor.authorTrudeau, VLen_HK
dc.date.accessioned2010-05-31T04:16:44Z-
dc.date.available2010-05-31T04:16:44Z-
dc.date.issued2009en_HK
dc.identifier.citationEndocrinology, 2009, v. 150 n. 5, p. 2273-2282en_HK
dc.identifier.issn0013-7227en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60699-
dc.description.abstractSecretoneurin (SN) isa 33-to34-aminoacid neuropeptide derived from secretogranin-II, a member of the chromogranin family. We previously synthesized a putative goldfish (gf) SN and demonstrated its ability to stimulate LH release in vivo. However, it was not known whether goldfish actually produced the free SN peptide or whether SN directly stimulates LH release from isolated pituitary cells. Using a combination of reverse-phase HPLC and mass spectrometry analysis, we isolated for the first time a 34-amino acid free gfSN peptide from the whole brain. Moreover, Western blot analysis indicated the existence of this peptide in goldfish pituitary. Immunocyto- chemical localization studies revealed the presence of SN immunoreactivity in prolactin cells of rostral pars distalis of the anterior pituitary. Additionally, we found that magnocellular cells of the goldfish preoptic region are highly immunoreactive for SN. These neurons send heavily labeled projections that pass through the pituitary stalk and innervate the neurointermediateand anterior lobes. In static 12-h incubation of dispersed pituitary cells, application of SN antiserum reduced LH levels, whereas 1 and 10 nM gfSN, respectively, induced 2.5-fold (P < 0.001) and 1.9-fold (P < 0.01) increments of LH release into the medium, increases similar to those elicited by 100 nM concentrations of GnRH. LikeGnRH, gfSN elevated intracellular Ca2+ in identified gonadotrophs. Whereas we do not yet know the relative contribution of neural SN or pituitary SN to LH release, we propose that SN could act as a neuroendocrine and/or paracrine factor to regulate LH release from the anterior pituitary. Copyright © 2009 by The Endocrine Society.en_HK
dc.languageengen_HK
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_HK
dc.relation.ispartofEndocrinologyen_HK
dc.rightsEndocrinology. Copyright © The Endocrine Society.en_HK
dc.titleThe secretogranin II-derived peptide secretoneurin stimulates luteinizing hormone secretion from gonadotrophsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0013-7227&volume=150&spage=2273&epage=2282&date=2009&atitle=The+secretogranin+II-derived+peptide+secretoneurin+stimulates+luteinizing+hormone+secretion+from+gonadotrophs+en_HK
dc.identifier.emailWong, AOL: olwong@hkucc.hku.hken_HK
dc.identifier.authorityWong, AOL=rp00806en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1210/en.2008-1060en_HK
dc.identifier.pmid19106223-
dc.identifier.scopuseid_2-s2.0-66449117778en_HK
dc.identifier.hkuros164818en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66449117778&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume150en_HK
dc.identifier.issue5en_HK
dc.identifier.spage2273en_HK
dc.identifier.epage2282en_HK
dc.identifier.isiWOS:000265407500032-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridZhao, E=7101850098en_HK
dc.identifier.scopusauthoridBasak, A=35848065700en_HK
dc.identifier.scopusauthoridWong, AOL=7403147570en_HK
dc.identifier.scopusauthoridKo, W=7202286890en_HK
dc.identifier.scopusauthoridChen, A=26641084700en_HK
dc.identifier.scopusauthoridLopez, GC=8507136000en_HK
dc.identifier.scopusauthoridGrey, CL=23102931800en_HK
dc.identifier.scopusauthoridCanosa, LF=6601938999en_HK
dc.identifier.scopusauthoridSomoza, GM=7003791807en_HK
dc.identifier.scopusauthoridChang, JP=7601547649en_HK
dc.identifier.scopusauthoridTrudeau, VL=7006634572en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats