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Article: Brassica juncea chitinase BjCHI1 inhibits growth of fungal phytopathogens and agglutinates Gram-negative bacteria

TitleBrassica juncea chitinase BjCHI1 inhibits growth of fungal phytopathogens and agglutinates Gram-negative bacteria
Authors
KeywordsBacterial agglutination
Chitin-binding domain
Chloroplast transformation
Indian mustard
Lectin
Phytopathogens
Pichia-expressed proteins
Transplastomic tobacco
Issue Date2008
PublisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/
Citation
Journal Of Experimental Botany, 2008, v. 59 n. 12, p. 3475-3484 How to Cite?
AbstractBrassica juncea BjCHI1 is a plant chitinase with two chitin-binding domains. Its expression, induced in response to wounding, methyl jasmonate treatment, Aspergillus niger infection, and caterpillar Pieris rapae feeding, suggests that it plays a role in defence. In this study, to investigate the potential of using BjCHI1 in agriculture, Pichia-expressed BjCHI1 and its deletion derivatives that lack one or both chitin-binding domains were tested against phytopathogenic fungi and bacteria. Transplastomic tobacco expressing BjCHI1 was also generated and its extracts assessed. In radial growth-inhibition assays, BjCHI1 and its derivative with one chitin-binding domain showed anti-fungal activities against phytopathogens, Colletotrichum truncatum, C. acutatum, Botrytis cinerea, and Ascochyta rabiei. BjCHI1 also inhibited spore germination of C. truncatum. Furthermore, BjCHI1, but not its derivatives lacking one or both domains, inhibited the growth of Gram-negative bacteria (Escherichia coli, Ralstonia solanacearum, Pseudomonas aeruginosa) more effectively than Gram-positive bacteria (Micrococcus luteus and Bacillus megaterium), indicating that the duplicated chitin-binding domain, uncommon in chitinases, is essential for bacterial agglutination. Galactose, glucose, and lactose relieved agglutination, suggesting that BjCHI1 interacts with the carbohydrate components of the Gram-negative bacterial cell wall. Retention of chitinase and bacterial agglutination activities in transplastomic tobacco extracts implicates that BjCHI1 is potentially useful against both fungal and bacterial phytopathogens in agriculture. © 2008 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/60690
ISSN
2015 Impact Factor: 5.677
2015 SCImago Journal Rankings: 2.798
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
University of Hong Kong10205761
10208034
Funding Information:

We thank Professor CY Ma for the provision of FPLC facilities. This work was supported the University of Hong Kong (10205761 and 10208034). MLC was recipient of a Dyason Universitas 21 Fellowship at the University of Melbourne.

References

 

DC FieldValueLanguage
dc.contributor.authorGuan, Yen_HK
dc.contributor.authorRamalingam, Sen_HK
dc.contributor.authorNagegowda, Den_HK
dc.contributor.authorTaylor, PWJen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-05-31T04:16:34Z-
dc.date.available2010-05-31T04:16:34Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Experimental Botany, 2008, v. 59 n. 12, p. 3475-3484en_HK
dc.identifier.issn0022-0957en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60690-
dc.description.abstractBrassica juncea BjCHI1 is a plant chitinase with two chitin-binding domains. Its expression, induced in response to wounding, methyl jasmonate treatment, Aspergillus niger infection, and caterpillar Pieris rapae feeding, suggests that it plays a role in defence. In this study, to investigate the potential of using BjCHI1 in agriculture, Pichia-expressed BjCHI1 and its deletion derivatives that lack one or both chitin-binding domains were tested against phytopathogenic fungi and bacteria. Transplastomic tobacco expressing BjCHI1 was also generated and its extracts assessed. In radial growth-inhibition assays, BjCHI1 and its derivative with one chitin-binding domain showed anti-fungal activities against phytopathogens, Colletotrichum truncatum, C. acutatum, Botrytis cinerea, and Ascochyta rabiei. BjCHI1 also inhibited spore germination of C. truncatum. Furthermore, BjCHI1, but not its derivatives lacking one or both domains, inhibited the growth of Gram-negative bacteria (Escherichia coli, Ralstonia solanacearum, Pseudomonas aeruginosa) more effectively than Gram-positive bacteria (Micrococcus luteus and Bacillus megaterium), indicating that the duplicated chitin-binding domain, uncommon in chitinases, is essential for bacterial agglutination. Galactose, glucose, and lactose relieved agglutination, suggesting that BjCHI1 interacts with the carbohydrate components of the Gram-negative bacterial cell wall. Retention of chitinase and bacterial agglutination activities in transplastomic tobacco extracts implicates that BjCHI1 is potentially useful against both fungal and bacterial phytopathogens in agriculture. © 2008 The Author(s).en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Experimental Botanyen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong Licenseen_HK
dc.rightsJournal of Experimental Botany. Copyright © Oxford University Press.-
dc.subjectBacterial agglutinationen_HK
dc.subjectChitin-binding domainen_HK
dc.subjectChloroplast transformationen_HK
dc.subjectIndian mustarden_HK
dc.subjectLectinen_HK
dc.subjectPhytopathogensen_HK
dc.subjectPichia-expressed proteinsen_HK
dc.subjectTransplastomic tobaccoen_HK
dc.titleBrassica juncea chitinase BjCHI1 inhibits growth of fungal phytopathogens and agglutinates Gram-negative bacteriaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0957&volume=59&spage=3475&epage=3484&date=2008&atitle=Brassica+juncea+chitinase+BjCHI1+inhibits+growth+of+fungal+phytopathogens+and+agglutinates+Gram-negative+bacteria.en_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1093/jxb/ern197en_HK
dc.identifier.pmid18669819-
dc.identifier.pmcidPMC2529242-
dc.identifier.scopuseid_2-s2.0-51749086433en_HK
dc.identifier.hkuros154124en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51749086433&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue12en_HK
dc.identifier.spage3475en_HK
dc.identifier.epage3484en_HK
dc.identifier.isiWOS:000259202900023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridGuan, Y=24802016400en_HK
dc.identifier.scopusauthoridRamalingam, S=8709830400en_HK
dc.identifier.scopusauthoridNagegowda, D=8709830800en_HK
dc.identifier.scopusauthoridTaylor, PWJ=24465972000en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK
dc.identifier.citeulike4040154-

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