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Conference Paper: Molecular cloning and mRNA Distribution of pituitary adenylate cyclase-activating polypeptide (PACAP)/PACAP-related peptide in the lungfish

TitleMolecular cloning and mRNA Distribution of pituitary adenylate cyclase-activating polypeptide (PACAP)/PACAP-related peptide in the lungfish
Authors
KeywordsLungfish
PACAP-related peptide
Pituitary adenylate cyclase-activating polypeptide
Issue Date2009
PublisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0077-8923&site=1
Citation
Conference on Trends in Comparative Endocrinology and Neurobiology, Genoa, Italy, 2-6 September 2008. In Annals Of The New York Academy Of Sciences, 2009, v. 1163, p. 209-214 How to Cite?
AbstractIn this article, we report the isolation of a full-length cDNA clone encoding pituitary adenylate cyclase-activating polypeptide (PACAP)/PACAP- related peptide (PRP) from lungfish Protopterus dolloi. When comparing the deduced amino acid sequences, the lungfish PACAP was found to be highly conserved with other vertebrates; however, the PRP shares only lower levels of sequence identity with known PRP sequences. Consistently in phylogenetic analysis, the lungfish PRP, similar to sturgeon PRP, fails to cluster with other PRPs. In addition to the full-length clone, another cDNA encoding a short precursor that lacks the first 32 amino acids of the PRP was also isolated. Interestingly, similar isoforms were also identified in several nonmammalian vertebrates, and it was suggested that exon skipping of PRP/PACAP transcripts was a mechanism that regulated the expression ratio of PACAP to PRP in nonmammalian vertebrates. By real-time PCR, both long and short PRP/PACAP transcripts were found almost exclusively in the brain, and the short isoform is the more abundant transcript (3.7 times more), indicating that PACAP is the major product produced in lungfish brain. The expression patterns of lungfish and previously studied frog PRP/PACAP suggest that the PRP/PACAP gene in the tetrapod lineage may first express in the central nervous system; in the process of evolution, the functions of these peptides diversified and were later found in other tissues. © 2009 New York Academy of Sciences.
Persistent Identifierhttp://hdl.handle.net/10722/60682
ISBN
ISSN
2015 Impact Factor: 4.518
2015 SCImago Journal Rankings: 2.389
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Government Research Grants CouncilHKU 7501/05M
CRCG10208128
Funding Information:

The present study was supported by the Hong Kong Government Research Grants Council grants HKU 7501/05M to B.K.C.C. and CRCG10208128 to L.T.O.L.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorLee, LTOen_HK
dc.contributor.authorTam, JKVen_HK
dc.contributor.authorChan, DWen_HK
dc.contributor.authorChow, BKCen_HK
dc.date.accessioned2010-05-31T04:16:26Z-
dc.date.available2010-05-31T04:16:26Z-
dc.date.issued2009en_HK
dc.identifier.citationConference on Trends in Comparative Endocrinology and Neurobiology, Genoa, Italy, 2-6 September 2008. In Annals Of The New York Academy Of Sciences, 2009, v. 1163, p. 209-214en_HK
dc.identifier.isbn9781573316712-
dc.identifier.issn0077-8923en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60682-
dc.description.abstractIn this article, we report the isolation of a full-length cDNA clone encoding pituitary adenylate cyclase-activating polypeptide (PACAP)/PACAP- related peptide (PRP) from lungfish Protopterus dolloi. When comparing the deduced amino acid sequences, the lungfish PACAP was found to be highly conserved with other vertebrates; however, the PRP shares only lower levels of sequence identity with known PRP sequences. Consistently in phylogenetic analysis, the lungfish PRP, similar to sturgeon PRP, fails to cluster with other PRPs. In addition to the full-length clone, another cDNA encoding a short precursor that lacks the first 32 amino acids of the PRP was also isolated. Interestingly, similar isoforms were also identified in several nonmammalian vertebrates, and it was suggested that exon skipping of PRP/PACAP transcripts was a mechanism that regulated the expression ratio of PACAP to PRP in nonmammalian vertebrates. By real-time PCR, both long and short PRP/PACAP transcripts were found almost exclusively in the brain, and the short isoform is the more abundant transcript (3.7 times more), indicating that PACAP is the major product produced in lungfish brain. The expression patterns of lungfish and previously studied frog PRP/PACAP suggest that the PRP/PACAP gene in the tetrapod lineage may first express in the central nervous system; in the process of evolution, the functions of these peptides diversified and were later found in other tissues. © 2009 New York Academy of Sciences.en_HK
dc.languageengen_HK
dc.publisherWiley-Blackwell Publishing, Inc. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0077-8923&site=1en_HK
dc.relation.ispartofAnnals of the New York Academy of Sciencesen_HK
dc.subjectLungfishen_HK
dc.subjectPACAP-related peptideen_HK
dc.subjectPituitary adenylate cyclase-activating polypeptideen_HK
dc.titleMolecular cloning and mRNA Distribution of pituitary adenylate cyclase-activating polypeptide (PACAP)/PACAP-related peptide in the lungfishen_HK
dc.typeConference_Paperen_HK
dc.identifier.emailLee, LTO: ltolee2@hkucc.hku.hken_HK
dc.identifier.emailChow, BKC: bkcc@hku.hken_HK
dc.identifier.authorityLee, LTO=rp00727en_HK
dc.identifier.authorityChow, BKC=rp00681en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1111/j.1749-6632.2008.03661.xen_HK
dc.identifier.pmid19456341-
dc.identifier.scopuseid_2-s2.0-65449179981en_HK
dc.identifier.hkuros156842en_HK
dc.identifier.hkuros181132-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-65449179981&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume1163en_HK
dc.identifier.spage209en_HK
dc.identifier.epage214en_HK
dc.identifier.isiWOS:000266493400018-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectSecretin: a putative neurosecretory hormone that regulates water homeostasis in the hypothalamus-pituitary-adrenal axis-
dc.identifier.scopusauthoridLee, LTO=8367269000en_HK
dc.identifier.scopusauthoridTam, JKV=36830083000en_HK
dc.identifier.scopusauthoridChan, DW=7402216783en_HK
dc.identifier.scopusauthoridChow, BKC=7102826193en_HK
dc.identifier.citeulike4449058-

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