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Article: Use of capillary electrophoresis to evaluate protective effects of methylglyoxal scavengers on the activity of creatine kinase

TitleUse of capillary electrophoresis to evaluate protective effects of methylglyoxal scavengers on the activity of creatine kinase
Authors
KeywordsCapillary electrophoresis
Creatine kinase
Methylglyoxal
Thiols
Tiopronin
Issue Date2008
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jss
Citation
Journal Of Separation Science, 2008, v. 31 n. 15, p. 2846-2851 How to Cite?
Abstract
Methylglyoxal (MGO) is a highly reactive α-oxoaldehyde formed endogenously in numerous enzymatic and nonenzymatic reactions. The reactions between MGO and various amino residues in proteins not only result in inactivation of enzymes, but also lead to the formation of different detrimental advanced glycation endproducts (AGEs). Recently, it was reported that creatine kinase (CK, EC 2.7.3.2) activity could be reduced or even lost under incubation with MGO in vitro. In this study, an efficient CE analytical method was developed for the evaluation of CK activity. Based on this CE method, the inhibitory effect of MGO on CK activity was confirmed. Several MGO scavengers such as aminoguanidine (AG) and some thiols showed obvious protective effects on CK activity against MGO. Furthermore, tiopronin (TP), a hepatoprotective drug, was found for the first time to counteract MGO-induced inhibition of CK activity in CK reaction. Meanwhile, TP also retained adenosine diphosphate (ADP) generation level in plasma treated with MGO, which implies that this drug may have potential protective effect on other enzymes which are associated with adenine nucleotide metabolism. Besides, the established CE approach can be utilized as a model for screening effective MGO scavengers by monitoring CK-catalyzed conversion between adenosine triphosphate and ADP. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhem.
Persistent Identifierhttp://hdl.handle.net/10722/60675
ISSN
2013 Impact Factor: 2.594
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, Jen_HK
dc.contributor.authorPeng, Xen_HK
dc.contributor.authorCheng, KWen_HK
dc.contributor.authorChen, Fen_HK
dc.contributor.authorYang, Den_HK
dc.contributor.authorChen, Ben_HK
dc.contributor.authorWang, MFen_HK
dc.date.accessioned2010-05-31T04:16:18Z-
dc.date.available2010-05-31T04:16:18Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Separation Science, 2008, v. 31 n. 15, p. 2846-2851en_HK
dc.identifier.issn1615-9306en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60675-
dc.description.abstractMethylglyoxal (MGO) is a highly reactive α-oxoaldehyde formed endogenously in numerous enzymatic and nonenzymatic reactions. The reactions between MGO and various amino residues in proteins not only result in inactivation of enzymes, but also lead to the formation of different detrimental advanced glycation endproducts (AGEs). Recently, it was reported that creatine kinase (CK, EC 2.7.3.2) activity could be reduced or even lost under incubation with MGO in vitro. In this study, an efficient CE analytical method was developed for the evaluation of CK activity. Based on this CE method, the inhibitory effect of MGO on CK activity was confirmed. Several MGO scavengers such as aminoguanidine (AG) and some thiols showed obvious protective effects on CK activity against MGO. Furthermore, tiopronin (TP), a hepatoprotective drug, was found for the first time to counteract MGO-induced inhibition of CK activity in CK reaction. Meanwhile, TP also retained adenosine diphosphate (ADP) generation level in plasma treated with MGO, which implies that this drug may have potential protective effect on other enzymes which are associated with adenine nucleotide metabolism. Besides, the established CE approach can be utilized as a model for screening effective MGO scavengers by monitoring CK-catalyzed conversion between adenosine triphosphate and ADP. © 2008 Wiley-VCH Verlag GmbH & Co. KGaA, Weinhem.en_HK
dc.languageengen_HK
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/jssen_HK
dc.relation.ispartofJournal of Separation Scienceen_HK
dc.subjectCapillary electrophoresisen_HK
dc.subjectCreatine kinaseen_HK
dc.subjectMethylglyoxalen_HK
dc.subjectThiolsen_HK
dc.subjectTioproninen_HK
dc.titleUse of capillary electrophoresis to evaluate protective effects of methylglyoxal scavengers on the activity of creatine kinaseen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1615-9306&volume=31&spage=2846&epage=51&date=2008&atitle=Use+of+capillary+electrophoresis+to+evaluate+protective+effects+of+methylglyoxal+scavengers+on+the+activity+of+creatine+kinase.en_HK
dc.identifier.emailChen, F: sfchen@hku.hken_HK
dc.identifier.emailWang, MF: mfwang@hku.hken_HK
dc.identifier.authorityChen, F=rp00672en_HK
dc.identifier.authorityWang, MF=rp00800en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/jssc.200800241en_HK
dc.identifier.pmid18655020en_HK
dc.identifier.scopuseid_2-s2.0-51549112193en_HK
dc.identifier.hkuros151445en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51549112193&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume31en_HK
dc.identifier.issue15en_HK
dc.identifier.spage2846en_HK
dc.identifier.epage2851en_HK
dc.identifier.isiWOS:000258936700013-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridMa, J=9248720900en_HK
dc.identifier.scopusauthoridPeng, X=23995738500en_HK
dc.identifier.scopusauthoridCheng, KW=12141247000en_HK
dc.identifier.scopusauthoridChen, F=7404907980en_HK
dc.identifier.scopusauthoridYang, D=12041062700en_HK
dc.identifier.scopusauthoridChen, B=22633788300en_HK
dc.identifier.scopusauthoridWang, MF=7406691844en_HK

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