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Article: Epidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium

TitleEpidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epithelium
Authors
KeywordsActin
Actin dynamics
Germ cells
Sertoli cells
Spermatogenesis
Issue Date2009
PublisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/
Citation
FASEB Journal, 2009, v. 23 n. 8, p. 2555-2567 How to Cite?
AbstractIn the seminiferous epithelium, Eps8 is localized to actin-based cell junctions at the blood-testis barrier (BTB) and the apical ectoplasmic specialization (ES) in stage V-VI tubules but is considerably diminished in stage VIII tubules. Eps8 down-regulation coincides with the time of BTB restructuring and apical ES disassembly, implicating the role of Eps8 in cell adhesion. Its involvement in Sertoli-germ cell adhesion was substantiated in studies using an in vivo animal model by treating rats with 1-(2,4- dichlorobenzy)-1H-indazole-3-carbohydrazide (adjudin) to induce anchoring junction restructuring, during which Eps8 disappeared at the apical ES before germ cell departure. In Sertoli cell cultures with established permeability barrier mimicking the BTB in vivo, the knockdown of Eps8 by RNAi led to F-actin disorganization and the mislocalization of the tight junction proteins occludin and ZO-1, suggesting the function of Eps8 in maintaining BTB integrity. In vivo knockdown of Eps8 in the testis caused germ cell sloughing and BTB damage, concomitant with occludin mislocalization, further validating that Eps8 is a novel regulator of cell adhesion and BTB integrity in the seminiferous epithelium. © FASEB.
Persistent Identifierhttp://hdl.handle.net/10722/60659
ISSN
2015 Impact Factor: 5.299
2015 SCImago Journal Rankings: 2.775
ISI Accession Number ID
Funding AgencyGrant Number
NIH (NICHD)R01HD056034
R03HD051512
Hong Kong Research Grants CouncilHKU7599/06M
Funding Information:

This work was supported by NIH grants (NICHD, R01HD056034, R03HD051512 to C.Y.C.) and a Hong Kong Research Grants Council grant (HKU7599/06M to W. M. L.).

References

 

DC FieldValueLanguage
dc.contributor.authorLie, PPYen_HK
dc.contributor.authorMruk, DDen_HK
dc.contributor.authorLee, WMen_HK
dc.contributor.authorYan Cheng, Cen_HK
dc.date.accessioned2010-05-31T04:16:00Z-
dc.date.available2010-05-31T04:16:00Z-
dc.date.issued2009en_HK
dc.identifier.citationFASEB Journal, 2009, v. 23 n. 8, p. 2555-2567en_HK
dc.identifier.issn0892-6638en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60659-
dc.description.abstractIn the seminiferous epithelium, Eps8 is localized to actin-based cell junctions at the blood-testis barrier (BTB) and the apical ectoplasmic specialization (ES) in stage V-VI tubules but is considerably diminished in stage VIII tubules. Eps8 down-regulation coincides with the time of BTB restructuring and apical ES disassembly, implicating the role of Eps8 in cell adhesion. Its involvement in Sertoli-germ cell adhesion was substantiated in studies using an in vivo animal model by treating rats with 1-(2,4- dichlorobenzy)-1H-indazole-3-carbohydrazide (adjudin) to induce anchoring junction restructuring, during which Eps8 disappeared at the apical ES before germ cell departure. In Sertoli cell cultures with established permeability barrier mimicking the BTB in vivo, the knockdown of Eps8 by RNAi led to F-actin disorganization and the mislocalization of the tight junction proteins occludin and ZO-1, suggesting the function of Eps8 in maintaining BTB integrity. In vivo knockdown of Eps8 in the testis caused germ cell sloughing and BTB damage, concomitant with occludin mislocalization, further validating that Eps8 is a novel regulator of cell adhesion and BTB integrity in the seminiferous epithelium. © FASEB.en_HK
dc.languageengen_HK
dc.publisherFederation of American Societies for Experimental Biology. The Journal's web site is located at http://www.fasebj.org/en_HK
dc.relation.ispartofFASEB Journalen_HK
dc.subjectActinen_HK
dc.subjectActin dynamicsen_HK
dc.subjectGerm cellsen_HK
dc.subjectSertoli cellsen_HK
dc.subjectSpermatogenesisen_HK
dc.titleEpidermal growth factor receptor pathway substrate 8 (Eps8) is a novel regulator of cell adhesion and the blood-testis barrier integrity in the seminiferous epitheliumen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0892-6638&volume=23&spage=2555&epage=2567&date=2009&atitle=Epidermal+growth+factor+receptor+pathway+substrate+8+(Eps8)+is+a+novel+regulator+of+cell+adhesion+and+the+blood-testis+barrier+integrity+in+the+seminiferous+epithelium.+en_HK
dc.identifier.emailLee, WM: hrszlwm@hku.hken_HK
dc.identifier.authorityLee, WM=rp00728en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1096/fj.06-070573en_HK
dc.identifier.pmid19293393-
dc.identifier.scopuseid_2-s2.0-68849115313en_HK
dc.identifier.hkuros164871en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-68849115313&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume23en_HK
dc.identifier.issue8en_HK
dc.identifier.spage2555en_HK
dc.identifier.epage2567en_HK
dc.identifier.isiWOS:000268836700024-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLie, PPY=15839862700en_HK
dc.identifier.scopusauthoridMruk, DD=6701823934en_HK
dc.identifier.scopusauthoridLee, WM=24799156600en_HK
dc.identifier.scopusauthoridYan Cheng, C=6602806090en_HK

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