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Article: Ethylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein

TitleEthylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding protein
Authors
KeywordsAcyl-CoA-binding protein
Ethylene
Pathogen
Protein-protein interaction
Issue Date2008
PublisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/
Citation
Journal Of Experimental Botany, 2008, v. 59 n. 14, p. 3997-4006 How to Cite?
AbstractSix genes encode proteins with acyl-CoA-binding domains in Arabidopsis thaliana. They are the small 10-kDa cytosolic acyl-CoA-binding protein (ACBP), membrane-associated ACBP1 and ACBP2, extracellularly-targeted ACBP3, and kelch-motif containing ACBP4 and ACBP5. Here, the interaction of ACBP4 with an A. thaliana ethylene-responsive element binding protein (AtEBP), identified in a yeast two-hybrid screen, was confirmed by co-immunoprecipitation. The subcellular localization of ACBP4 and AtEBP, was addressed using an ACBP4:DsRed red fluorescent protein fusion and a green fluorescent protein (GFP):AtEBP fusion. Transient expression of these autofluoresence-tagged proteins in agroinfiltrated tobacco leaves, followed by confocal laser scanning microscopy, indicated their co-localization predominantly at the cytosol which was confirmed by FRET analysis. Immuno-electron microscopy on Arabidopsis sections not only localized ACBP4 to the cytosol but also to the periphery of the nucleus upon closer examination, perhaps as a result of its interaction with AtEBP. Furthermore, the expression of ACBP4 and AtEBP in Northern blot analyses was induced by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, methyl jasmonate treatments, and Botrytis cinerea infection, suggesting that the interaction of ACBP4 and AtEBP may be related to AtEBP-mediated defence possibly via ethylene and/or jasmonate signalling. © 2008 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/60637
ISSN
2015 Impact Factor: 5.677
2015 SCImago Journal Rankings: 2.798
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Special Administrative Region, ChinaHKU7314/04M
HKU7504/05M
University of Hong Kong
Croucher Foundation
Funding Information:

We thank WL Crosby (Plant Biotechnology Institute, NRC, Canada) for provision of the yeast two-hybrid system; MM Goodin (University of California, Berkeley) for provision of vectors in the construction of autofluorescent protein fusions; and GSW Tsao (The University of Hong Kong) for provision of the Zeiss LSM510 META. This work was supported by the Research Grants Council of the Hong Kong Special Administrative Region, China (projects HKU7314/04M and HKU7504/05M). SX was supported by a postgraduate studentship from the University of Hong Kong and HY Li by the Croucher Foundation.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, HYen_HK
dc.contributor.authorXiao, Sen_HK
dc.contributor.authorChye, MLen_HK
dc.date.accessioned2010-05-31T04:15:35Z-
dc.date.available2010-05-31T04:15:35Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Experimental Botany, 2008, v. 59 n. 14, p. 3997-4006en_HK
dc.identifier.issn0022-0957en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60637-
dc.description.abstractSix genes encode proteins with acyl-CoA-binding domains in Arabidopsis thaliana. They are the small 10-kDa cytosolic acyl-CoA-binding protein (ACBP), membrane-associated ACBP1 and ACBP2, extracellularly-targeted ACBP3, and kelch-motif containing ACBP4 and ACBP5. Here, the interaction of ACBP4 with an A. thaliana ethylene-responsive element binding protein (AtEBP), identified in a yeast two-hybrid screen, was confirmed by co-immunoprecipitation. The subcellular localization of ACBP4 and AtEBP, was addressed using an ACBP4:DsRed red fluorescent protein fusion and a green fluorescent protein (GFP):AtEBP fusion. Transient expression of these autofluoresence-tagged proteins in agroinfiltrated tobacco leaves, followed by confocal laser scanning microscopy, indicated their co-localization predominantly at the cytosol which was confirmed by FRET analysis. Immuno-electron microscopy on Arabidopsis sections not only localized ACBP4 to the cytosol but also to the periphery of the nucleus upon closer examination, perhaps as a result of its interaction with AtEBP. Furthermore, the expression of ACBP4 and AtEBP in Northern blot analyses was induced by the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, methyl jasmonate treatments, and Botrytis cinerea infection, suggesting that the interaction of ACBP4 and AtEBP may be related to AtEBP-mediated defence possibly via ethylene and/or jasmonate signalling. © 2008 The Author(s).en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://jxb.oxfordjournals.org/en_HK
dc.relation.ispartofJournal of Experimental Botanyen_HK
dc.rightsJournal of Experimental Botany. Copyright © Oxford University Press.en_HK
dc.subjectAcyl-CoA-binding proteinen_HK
dc.subjectEthyleneen_HK
dc.subjectPathogenen_HK
dc.subjectProtein-protein interactionen_HK
dc.titleEthylene- and pathogen-inducible Arabidopsis acyl-CoA-binding protein 4 interacts with an ethylene-responsive element binding proteinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-0957&volume=59&spage=3997&epage=4006&date=2008&atitle=Ethylene-and+pathogen-inducible+Arabidopsis+acyl-CoA-binding+protein+4+interacts+with+an+ethylene-responsive+element+binding+proteinen_HK
dc.identifier.emailXiao, S: xiaoshi@graduate.hku.hken_HK
dc.identifier.emailChye, ML: mlchye@hkucc.hku.hken_HK
dc.identifier.authorityXiao, S=rp00817en_HK
dc.identifier.authorityChye, ML=rp00687en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/jxb/ern241en_HK
dc.identifier.pmid18836139-
dc.identifier.scopuseid_2-s2.0-55249127541en_HK
dc.identifier.hkuros154140en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-55249127541&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume59en_HK
dc.identifier.issue14en_HK
dc.identifier.spage3997en_HK
dc.identifier.epage4006en_HK
dc.identifier.isiWOS:000260556800018-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, HY=22953303900en_HK
dc.identifier.scopusauthoridXiao, S=7402022635en_HK
dc.identifier.scopusauthoridChye, ML=7003905460en_HK

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