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Article: Promoter-sharing by different genes in human genome - CPNE1 and RBM12 gene pair as an example

TitlePromoter-sharing by different genes in human genome - CPNE1 and RBM12 gene pair as an example
Authors
Issue Date2008
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgenomics/
Citation
Bmc Genomics, 2008, v. 9 How to Cite?
AbstractBackground: Regulation of gene expression plays important role in cellular functions. Co-regulation of different genes may indicate functional connection or even physical interaction between gene products. Thus analysis on genomic structures that may affect gene expression regulation could shed light on the functions of genes. Results: In a whole genome analysis of alternative splicing events, we found that two distinct genes, copine I (CPNE1) and RNA binding motif protein 12 (RBM12), share the most 5′ exons and therefore the promoter region in human. Further analysis identified many gene pairs in human genome that share the same promoters and 5′ exons but have totally different coding sequences. Analysis of genomic and expressed sequences, either cDNAs or expressed sequence tags (ESTs) for CPNE1 and RBM12, confirmed the conservation of this phenomenon during evolutionary courses. The co-expression of the two genes initiated from the same promoter is confirmed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) in different tissues in both human and mouse. High degrees of sequence conservation among multiple species in the 5′UTR region common to CPNE1 and RBM12 were also identified. Conclusion: Promoter and 5′UTR sharing between CPNE1 and RBM12 is observed in human, mouse and zebrafish. Conservation of this genomic structure in evolutionary courses indicates potential functional interaction between the two genes. More than 20 other gene pairs in human genome were found to have the similar genomic structure in a genome-wide analysis, and it may represent a unique pattern of genomic arrangement that may affect expression regulation of the corresponding genes. © 2008 Yang et al; licensee BioMed Central Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/60620
ISSN
2015 Impact Factor: 3.867
2015 SCImago Journal Rankings: 2.343
ISI Accession Number ID
Funding AgencyGrant Number
University Research Committee
LKS Faculty of Medicine of the University of Hong Kong, Hong Kong, China
Edward Sai Kim Hotung Paediatric Education and Research Fund
University Postgraduate Studentship
Funding Information:

WY acknowledges financial support from University Research Committee and LKS Faculty of Medicine of the University of Hong Kong, Hong Kong, China. PN and MZ are supported by Edward Sai Kim Hotung Paediatric Education and Research Fund and University Postgraduate Studentship.

References

 

DC FieldValueLanguage
dc.contributor.authorYang, Wen_HK
dc.contributor.authorNg, Pen_HK
dc.contributor.authorZhao, Men_HK
dc.contributor.authorWong, TKFen_HK
dc.contributor.authorYiu, SMen_HK
dc.contributor.authorLau, YLen_HK
dc.date.accessioned2010-05-31T04:15:07Z-
dc.date.available2010-05-31T04:15:07Z-
dc.date.issued2008en_HK
dc.identifier.citationBmc Genomics, 2008, v. 9en_HK
dc.identifier.issn1471-2164en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60620-
dc.description.abstractBackground: Regulation of gene expression plays important role in cellular functions. Co-regulation of different genes may indicate functional connection or even physical interaction between gene products. Thus analysis on genomic structures that may affect gene expression regulation could shed light on the functions of genes. Results: In a whole genome analysis of alternative splicing events, we found that two distinct genes, copine I (CPNE1) and RNA binding motif protein 12 (RBM12), share the most 5′ exons and therefore the promoter region in human. Further analysis identified many gene pairs in human genome that share the same promoters and 5′ exons but have totally different coding sequences. Analysis of genomic and expressed sequences, either cDNAs or expressed sequence tags (ESTs) for CPNE1 and RBM12, confirmed the conservation of this phenomenon during evolutionary courses. The co-expression of the two genes initiated from the same promoter is confirmed by Reverse Transcription-Polymerase Chain Reaction (RT-PCR) in different tissues in both human and mouse. High degrees of sequence conservation among multiple species in the 5′UTR region common to CPNE1 and RBM12 were also identified. Conclusion: Promoter and 5′UTR sharing between CPNE1 and RBM12 is observed in human, mouse and zebrafish. Conservation of this genomic structure in evolutionary courses indicates potential functional interaction between the two genes. More than 20 other gene pairs in human genome were found to have the similar genomic structure in a genome-wide analysis, and it may represent a unique pattern of genomic arrangement that may affect expression regulation of the corresponding genes. © 2008 Yang et al; licensee BioMed Central Ltd.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.biomedcentral.com/bmcgenomics/en_HK
dc.relation.ispartofBMC Genomicsen_HK
dc.rightsB M C Genomics. Copyright © BioMed Central Ltd.en_HK
dc.subject.mesh5' Untranslated Regions - geneticsen_HK
dc.subject.meshAlternative Splicingen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshCarrier Proteins - geneticsen_HK
dc.subject.meshConserved Sequenceen_HK
dc.subject.meshDNA, Complementary - geneticsen_HK
dc.subject.meshExonsen_HK
dc.subject.meshExpressed Sequence Tagsen_HK
dc.subject.meshGene Expression Regulationen_HK
dc.subject.meshGenome, Humanen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred C57BLen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshPhylogenyen_HK
dc.subject.meshPromoter Regions, Geneticen_HK
dc.subject.meshRNA - geneticsen_HK
dc.subject.meshRNA-Binding Proteins - geneticsen_HK
dc.subject.meshSequence Alignmenten_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.subject.meshSyntenyen_HK
dc.subject.meshZebrafish - geneticsen_HK
dc.titlePromoter-sharing by different genes in human genome - CPNE1 and RBM12 gene pair as an exampleen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1471-2164&volume=9&spage=456&epage=&date=2008&atitle=Promoter-sharing+by+different+genes+in+human+genome+-+CPNE1+and+RBM12+gene+pair+as+an+exampleen_HK
dc.identifier.emailYang, W:yangwl@hkucc.hku.hken_HK
dc.identifier.emailYiu, SM:smyiu@cs.hku.hken_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.authorityYang, W=rp00524en_HK
dc.identifier.authorityYiu, SM=rp00207en_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1186/1471-2164-9-456en_HK
dc.identifier.pmid18831769-
dc.identifier.scopuseid_2-s2.0-54049102047en_HK
dc.identifier.hkuros155944en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-54049102047&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume9en_HK
dc.identifier.isiWOS:000260173800001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, W=23101349500en_HK
dc.identifier.scopusauthoridNg, P=36658519100en_HK
dc.identifier.scopusauthoridZhao, M=13309644600en_HK
dc.identifier.scopusauthoridWong, TKF=25423289800en_HK
dc.identifier.scopusauthoridYiu, SM=7003282240en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.citeulike3368811-

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