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Article: Identification of novel epstein-barr virus MicroRNA genes from nasopharyngeal carcinomas

TitleIdentification of novel epstein-barr virus MicroRNA genes from nasopharyngeal carcinomas
Authors
Issue Date2009
PublisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/
Citation
Journal Of Virology, 2009, v. 83 n. 7, p. 3333-3341 How to Cite?
AbstractMicroRNAs (miRNAs) represent a conserved class of small noncoding RNAs that are found in all higher eukaryotes as well as some DNA viruses. miRNAs are 20 to 25 nucleotides in length and have important regulatory functions in biological processes such as embryonic development, cell differentiation, hormone secretion, and metabolism. Furthermore, miRNAs have been implicated in the pathology of various diseases, including cancer. miRNA expression profiles not only classify different types of cancer but also may even help to characterize distinct tumor stages, therefore constituting a valuable tool for prognosis. Here we report the miRNA profile of Epstein-Barr virus (EBV)-positive nasopharyngeal carcinoma (NPC) tissue samples characterized by cloning and sequencing. We found that all EBV miRNAs from the BART region are expressed in NPC tissues, whereas EBV miRNAs from the BHRF1 region are not found. Moreover, we identified two novel EBV miRNA genes originating from the BART region that have not been found in other tissues or cell lines before. We also identified three new human miRNAs which might be specific for nasopharyngeal tissues. We further show that a number of different cellular miRNAs, including miR-15a and miR-16, are upor downregulated in NPC tissues compared to control tissues. We found that the tumor suppressor BRCA-1 is a target of miR-15a as well as miR-16, suggesting a miRNA role in NPC pathogenesis. Copyright © 2009, American Society for Microbiology.
Persistent Identifierhttp://hdl.handle.net/10722/60584
ISSN
2015 Impact Factor: 4.606
2015 SCImago Journal Rankings: 3.347
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Max-Planck Society
Deutsche Krebshilfe107166
Deutsche ForschungsgemeinschaftGR950/11-2
Funding Information:

This work was supported by the Max-Planck Society, in part by a grant from the Deutsche Krebshilfe (grant 107166 to G. M. and F. G.), and by Deutsche Forschungsgemeinschaft grant GR950/11-2 (to F. G.).

References

 

DC FieldValueLanguage
dc.contributor.authorZhu, JYen_HK
dc.contributor.authorPfuhl, Ten_HK
dc.contributor.authorMotsch, Nen_HK
dc.contributor.authorBarth, Sen_HK
dc.contributor.authorNicholls, Jen_HK
dc.contributor.authorGrässer, Fen_HK
dc.contributor.authorMeister, Gen_HK
dc.date.accessioned2010-05-31T04:14:11Z-
dc.date.available2010-05-31T04:14:11Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of Virology, 2009, v. 83 n. 7, p. 3333-3341en_HK
dc.identifier.issn0022-538Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/60584-
dc.description.abstractMicroRNAs (miRNAs) represent a conserved class of small noncoding RNAs that are found in all higher eukaryotes as well as some DNA viruses. miRNAs are 20 to 25 nucleotides in length and have important regulatory functions in biological processes such as embryonic development, cell differentiation, hormone secretion, and metabolism. Furthermore, miRNAs have been implicated in the pathology of various diseases, including cancer. miRNA expression profiles not only classify different types of cancer but also may even help to characterize distinct tumor stages, therefore constituting a valuable tool for prognosis. Here we report the miRNA profile of Epstein-Barr virus (EBV)-positive nasopharyngeal carcinoma (NPC) tissue samples characterized by cloning and sequencing. We found that all EBV miRNAs from the BART region are expressed in NPC tissues, whereas EBV miRNAs from the BHRF1 region are not found. Moreover, we identified two novel EBV miRNA genes originating from the BART region that have not been found in other tissues or cell lines before. We also identified three new human miRNAs which might be specific for nasopharyngeal tissues. We further show that a number of different cellular miRNAs, including miR-15a and miR-16, are upor downregulated in NPC tissues compared to control tissues. We found that the tumor suppressor BRCA-1 is a target of miR-15a as well as miR-16, suggesting a miRNA role in NPC pathogenesis. Copyright © 2009, American Society for Microbiology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society for Microbiology. The Journal's web site is located at http://jvi.asm.org/en_HK
dc.relation.ispartofJournal of Virologyen_HK
dc.rightsJournal of Virology. Copyright © American Society for Microbiology.en_HK
dc.subject.meshCarcinoma - virologyen_HK
dc.subject.meshCloning, Molecularen_HK
dc.subject.meshHerpesvirus 4, Human - geneticsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMicroRNAs - biosynthesis - genetics - isolation & purificationen_HK
dc.subject.meshNasopharyngeal Neoplasms - virologyen_HK
dc.subject.meshRNA, Viral - biosynthesis - genetics - isolation & purificationen_HK
dc.subject.meshSequence Analysis, DNAen_HK
dc.titleIdentification of novel epstein-barr virus MicroRNA genes from nasopharyngeal carcinomasen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0022-538X&volume=83&issue=7&spage=3333&epage=41&date=2009&atitle=Identification+of+novel+Epstein-Barr+virus+microRNA+genes+from+nasopharyngeal+carcinomasen_HK
dc.identifier.emailNicholls, J:nicholls@pathology.hku.hken_HK
dc.identifier.authorityNicholls, J=rp00364en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1128/JVI.01689-08en_HK
dc.identifier.pmid19144710-
dc.identifier.pmcidPMC2655542-
dc.identifier.scopuseid_2-s2.0-63149105412en_HK
dc.identifier.hkuros162148en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63149105412&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume83en_HK
dc.identifier.issue7en_HK
dc.identifier.spage3333en_HK
dc.identifier.epage3341en_HK
dc.identifier.isiWOS:000264046000049-
dc.publisher.placeUnited Statesen_HK
dc.identifier.citeulike3891909-

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