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Article: Mantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report

TitleMantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case report
Authors
KeywordsCD5+ plasma cells
Flow cytometry
Mantle cell lymphoma
Plasmacytic differentiation
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0196-4763:1/
Citation
Cytometry Part B - Clinical Cytometry, 2009, v. 76 n. 3, p. 218-224 How to Cite?
AbstractBackground: Plasmacytic differentiation in mantle cell lymphoma (MCL) occurs rarely. However, no flow cytometric studies that demonstrate plasmacytic (PC) differentiation in MCL have been reported. Herein, we report a case of MCL with PC differentiation identified by flow cytometry. Methods: Morphologic review was performed by hematoxylin and eosin (H&E) stained sections from paraffin-embedded lymph node, colon and bone marrow specimens, and Wright-Geimsa stained bone marrow aspirate smears and touch imprints. Immunohistochemical stains using antibodies against CD3, CD5, CD20, and cyclin-D1, and in-situ hybridization for kappa and lambda light chains were reviewed. Multicolor flow cytometry analysis was performed on the bone marrow aspirate with monoclonal antibodies to CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD23, CD38, CD45, CD56, CD138, and kappa and lambda light chains. FISH analysis for t(11; 14)(q13;q32) was performed on interphase cells. Results: The neoplastic cells had the cytologic features of MCL with nodal, bone marrow, and colonic involvement. In-situ hybridization for kappa and lambda light chains demonstrated clonal plasma cells in the lymph node and bone marrow biopsies. In addition, flow cytometric studies of the bone marrow aspirate showed three populations of neoplastic cells: a clonal B-cell population with typical MCL phenotype, a similar B-cell population in transition to plasma cells, and a clonal plasma cell population. The plasma cells retained CD5 expression and had the same light chain restriction as the clonal B-cells. Conclusions: Multi-parameter flow cytometry can be useful in demonstrating clonal PC differentiation in MCL and distinguishing from a concurrent but unrelated plasma cell dyscrasia. © 2008 Clinical Cytometry Society.
Persistent Identifierhttp://hdl.handle.net/10722/60573
ISSN
2015 Impact Factor: 2.822
2015 SCImago Journal Rankings: 0.936
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorNaushad, Hen_HK
dc.contributor.authorChoi, WWLen_HK
dc.contributor.authorPage, CJen_HK
dc.contributor.authorSanger, WGen_HK
dc.contributor.authorWeisenburger, DDen_HK
dc.contributor.authorAoun, Pen_HK
dc.date.accessioned2010-05-31T04:13:57Z-
dc.date.available2010-05-31T04:13:57Z-
dc.date.issued2009en_HK
dc.identifier.citationCytometry Part B - Clinical Cytometry, 2009, v. 76 n. 3, p. 218-224en_HK
dc.identifier.issn1552-4949en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60573-
dc.description.abstractBackground: Plasmacytic differentiation in mantle cell lymphoma (MCL) occurs rarely. However, no flow cytometric studies that demonstrate plasmacytic (PC) differentiation in MCL have been reported. Herein, we report a case of MCL with PC differentiation identified by flow cytometry. Methods: Morphologic review was performed by hematoxylin and eosin (H&E) stained sections from paraffin-embedded lymph node, colon and bone marrow specimens, and Wright-Geimsa stained bone marrow aspirate smears and touch imprints. Immunohistochemical stains using antibodies against CD3, CD5, CD20, and cyclin-D1, and in-situ hybridization for kappa and lambda light chains were reviewed. Multicolor flow cytometry analysis was performed on the bone marrow aspirate with monoclonal antibodies to CD3, CD4, CD5, CD8, CD14, CD19, CD20, CD23, CD38, CD45, CD56, CD138, and kappa and lambda light chains. FISH analysis for t(11; 14)(q13;q32) was performed on interphase cells. Results: The neoplastic cells had the cytologic features of MCL with nodal, bone marrow, and colonic involvement. In-situ hybridization for kappa and lambda light chains demonstrated clonal plasma cells in the lymph node and bone marrow biopsies. In addition, flow cytometric studies of the bone marrow aspirate showed three populations of neoplastic cells: a clonal B-cell population with typical MCL phenotype, a similar B-cell population in transition to plasma cells, and a clonal plasma cell population. The plasma cells retained CD5 expression and had the same light chain restriction as the clonal B-cells. Conclusions: Multi-parameter flow cytometry can be useful in demonstrating clonal PC differentiation in MCL and distinguishing from a concurrent but unrelated plasma cell dyscrasia. © 2008 Clinical Cytometry Society.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0196-4763:1/en_HK
dc.relation.ispartofCytometry Part B - Clinical Cytometryen_HK
dc.rightsCytometry. Part B: Clinical Cytometry. Copyright © John Wiley & Sons, Inc..-
dc.rightsSpecial Statement for Preprint only Before publication: 'This is a preprint of an article accepted for publication in [The Journal of Pathology] Copyright © ([year]) ([Pathological Society of Great Britain and Ireland])'. After publication: the preprint notice should be amended to follows: 'This is a preprint of an article published in [include the complete citation information for the final version of the Contribution as published in the print edition of the Journal]' For Cochrane Library/ Cochrane Database of Systematic Reviews, add statement & acknowledgement : ‘This review is published as a Cochrane Review in the Cochrane Database of Systematic Reviews 20XX, Issue X. Cochrane Reviews are regularly updated as new evidence emerges and in response to comments and criticisms, and the Cochrane Database of Systematic Reviews should be consulted for the most recent version of the Review.’ Please include reference to the Review and hyperlink to the original version using the following format e.g. Authors. Title of Review. Cochrane Database of Systematic Reviews 20XX, Issue #. Art. No.: CD00XXXX. DOI: 10.1002/14651858.CD00XXXX (insert persistent link to the article by using the URL: http://dx.doi.org/10.1002/14651858.CD00XXXX) (This statement should refer to the most recent issue of the Cochrane Database of Systematic Reviews in which the Review published.)-
dc.subjectCD5+ plasma cellsen_HK
dc.subjectFlow cytometryen_HK
dc.subjectMantle cell lymphomaen_HK
dc.subjectPlasmacytic differentiationen_HK
dc.subject.meshCell Differentiation-
dc.subject.meshImmunohistochemistry-
dc.subject.meshLymphoma, Mantle-Cell - pathology-
dc.subject.meshPlasma Cells - pathology-
dc.subject.meshSensitivity and Specificity-
dc.titleMantle cell lymphoma with flow cytometric evidence of clonal plasmacytic differentiation: A case reporten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1552-4949&volume=76&issue=3&spage=218&epage=224&date=2009&atitle=Mantle+cell+lymphoma+with+flow+cytometric+evidence+of+clonal+plasmacytic+differentiation:+a+case+report-
dc.identifier.emailChoi, WWL:wlchoi@pathology.hku.hken_HK
dc.identifier.authorityChoi, WWL=rp00247en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1002/cyto.b.20463en_HK
dc.identifier.pmid19061249-
dc.identifier.scopuseid_2-s2.0-66949179609en_HK
dc.identifier.hkuros160960en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-66949179609&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume76en_HK
dc.identifier.issue3en_HK
dc.identifier.spage218en_HK
dc.identifier.epage224en_HK
dc.identifier.isiWOS:000265371500009-
dc.publisher.placeUnited Statesen_HK

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