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Article: Effects of native human zona pellucida glycoproteins 3 and 4 on acrosome reaction and zona pellucida binding of human spermatozoa

TitleEffects of native human zona pellucida glycoproteins 3 and 4 on acrosome reaction and zona pellucida binding of human spermatozoa
Authors
KeywordsAcrosome reaction
Fertilization
Sperm
Zona pellucida
Issue Date2008
PublisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/
Citation
Biology Of Reproduction, 2008, v. 79 n. 5, p. 869-877 How to Cite?
AbstractAcrosome reaction is crucial to the penetration of spermatozoa through the zona pellucida (ZP). Glycosylation of ZP glycoproteins is important in spermatozoa-ZP interaction. Human ZP glycoprotein-3 (ZP3) is believed to initiate acrosome reaction. Recently, human ZP4 was also implicated in inducing acrosome reaction. These studies were based on recombinant human ZP proteins with glycosylation different from their native counterparts. In the present study, the effects of native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding were investigated. Native human ZP3 and ZP4 were immunoaffinity-purified. They induced acrosome reaction and inhibited spermatozoa-ZP binding time- and dose-dependently to different extents. These biological activities of human ZP3 and ZP4 depended partly on their glycosylation, with N-linked glycosylation contributing much more significantly than O-linked glycosylation. Studies with inhibitors showed that both human ZP3- and ZP4-induced acrosome reactions were protein kinase-C, protein tyrosine kinase, T-type Ca2+ channels, and extracellular Ca2+ dependent. G-protein also participated in human ZP3- but not in ZP4-induced acrosome reaction. On the other hand, protein kinase-A and L-type Ca 2+ channels took part only in human ZP4-induced acrosome reaction. This manuscript describes for the first time the actions of purified native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding. © 2008 by the Society for the Study of Reproduction, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/60394
ISSN
2014 Impact Factor: 3.318
2014 SCImago Journal Rankings: 1.397
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorWong, BSTen_HK
dc.contributor.authorChung, MKen_HK
dc.contributor.authorLam, KKWen_HK
dc.contributor.authorPang, RTKen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorSumitro, SBen_HK
dc.contributor.authorGupta, SKen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-05-31T04:09:45Z-
dc.date.available2010-05-31T04:09:45Z-
dc.date.issued2008en_HK
dc.identifier.citationBiology Of Reproduction, 2008, v. 79 n. 5, p. 869-877en_HK
dc.identifier.issn0006-3363en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60394-
dc.description.abstractAcrosome reaction is crucial to the penetration of spermatozoa through the zona pellucida (ZP). Glycosylation of ZP glycoproteins is important in spermatozoa-ZP interaction. Human ZP glycoprotein-3 (ZP3) is believed to initiate acrosome reaction. Recently, human ZP4 was also implicated in inducing acrosome reaction. These studies were based on recombinant human ZP proteins with glycosylation different from their native counterparts. In the present study, the effects of native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding were investigated. Native human ZP3 and ZP4 were immunoaffinity-purified. They induced acrosome reaction and inhibited spermatozoa-ZP binding time- and dose-dependently to different extents. These biological activities of human ZP3 and ZP4 depended partly on their glycosylation, with N-linked glycosylation contributing much more significantly than O-linked glycosylation. Studies with inhibitors showed that both human ZP3- and ZP4-induced acrosome reactions were protein kinase-C, protein tyrosine kinase, T-type Ca2+ channels, and extracellular Ca2+ dependent. G-protein also participated in human ZP3- but not in ZP4-induced acrosome reaction. On the other hand, protein kinase-A and L-type Ca 2+ channels took part only in human ZP4-induced acrosome reaction. This manuscript describes for the first time the actions of purified native human ZP3 and ZP4 on acrosome reaction and spermatozoa-ZP binding. © 2008 by the Society for the Study of Reproduction, Inc.en_HK
dc.languageengen_HK
dc.publisherSociety for the Study of Reproduction. The Journal's web site is located at http://www.biolreprod.org/en_HK
dc.relation.ispartofBiology of Reproductionen_HK
dc.subjectAcrosome reactionen_HK
dc.subjectFertilizationen_HK
dc.subjectSpermen_HK
dc.subjectZona pellucidaen_HK
dc.titleEffects of native human zona pellucida glycoproteins 3 and 4 on acrosome reaction and zona pellucida binding of human spermatozoaen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-3363&volume=79&spage=869&epage=877&date=2008&atitle=Effects+of+Native+Human+Zona+Pellucida+Glycoproteins+3+and+4+on+Acrosome+Reaction+and+Zona+Pellucida+Binding+of+Human+Spermatozoaen_HK
dc.identifier.emailChiu, PCN: pchiucn@hku.hken_HK
dc.identifier.emailPang, RTK: rtkpang@hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityPang, RTK=rp01761en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1095/biolreprod.108.069344en_HK
dc.identifier.pmid18667750en_HK
dc.identifier.scopuseid_2-s2.0-55549122993en_HK
dc.identifier.hkuros154738en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-55549122993&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume79en_HK
dc.identifier.issue5en_HK
dc.identifier.spage869en_HK
dc.identifier.epage877en_HK
dc.identifier.isiWOS:000260321300010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridWong, BST=24337009700en_HK
dc.identifier.scopusauthoridChung, MK=8964203600en_HK
dc.identifier.scopusauthoridLam, KKW=25637362300en_HK
dc.identifier.scopusauthoridPang, RTK=7004376636en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridSumitro, SB=55218596400en_HK
dc.identifier.scopusauthoridGupta, SK=55495151900en_HK
dc.identifier.scopusauthoridYeung, WSB=55763794887en_HK
dc.identifier.citeulike3181284-

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