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Article: Regulation of complement-3 protein expression in human and mouse oviducts

TitleRegulation of complement-3 protein expression in human and mouse oviducts
Authors
Issue Date2009
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692
Citation
Molecular Reproduction And Development, 2009, v. 76 n. 3, p. 301-308 How to Cite?
AbstractThe human oviduct derived embryotrophic factor-3 (ETF-3) contains complement protein-3 (C3) and its derivates. Although C3 is not embryotrophic, it is converted into the embryotrophic derivative, iC3b in the presence of embryos and oviductal cells. The regulation of C3 production in the oviduct is not known. The objectives of this study were to investigate the effects of presence of preimplantation embryos and hormones on C3 expression in the oviducts in vitro and in vivo. The expression of C3 in the oviduct of pregnant mice was compared to that of pseudo-pregnant mice. The hormonal action on C3 expression was studied in the ovariectomized mouse oviducts and human oviductal epithelial (OE) cells. The results showed that the level of C3 mRNA in the mouse oviduct was high on day 1 and Day 2, but decreased toa minimum on day 4 of pregnancy, whereas that of pseudo-pregnancy remained relatively stable within the same period. The protein levels of C3 and iC3b specific fragments, α-115 and α-40, respectively in the mouse oviductal luminal fluid were highest on day 3 of pregnancy, when the embryos were expected to be most sensitive to the embryotrophic activity of ETF-3. Estrogen elevated C3 expression in the ovariectomized mouse oviduct and the OE cells. Progesterone suppressed estrogen-induced C3 expression in the mouse oviduct, but had no effect on OE cells. In conclusion, the presence of embryo and steroid hormones regulate the synthesis and secretion of oviductal C3. © 2008 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/60383
ISSN
2015 Impact Factor: 2.141
2015 SCImago Journal Rankings: 1.041
ISI Accession Number ID
Funding AgencyGrant Number
Research Grant Council, Hong KongHKU7319/01M
HKU7411/04M
Funding Information:

This work was supported in part by grants (HKU7319/01M to WSBY and HKU7411/04M to KFL) from the Research Grant Council, Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorLee, YLen_HK
dc.contributor.authorCheong, AWYen_HK
dc.contributor.authorChow, WNen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2010-05-31T04:09:33Z-
dc.date.available2010-05-31T04:09:33Z-
dc.date.issued2009en_HK
dc.identifier.citationMolecular Reproduction And Development, 2009, v. 76 n. 3, p. 301-308en_HK
dc.identifier.issn1040-452Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/60383-
dc.description.abstractThe human oviduct derived embryotrophic factor-3 (ETF-3) contains complement protein-3 (C3) and its derivates. Although C3 is not embryotrophic, it is converted into the embryotrophic derivative, iC3b in the presence of embryos and oviductal cells. The regulation of C3 production in the oviduct is not known. The objectives of this study were to investigate the effects of presence of preimplantation embryos and hormones on C3 expression in the oviducts in vitro and in vivo. The expression of C3 in the oviduct of pregnant mice was compared to that of pseudo-pregnant mice. The hormonal action on C3 expression was studied in the ovariectomized mouse oviducts and human oviductal epithelial (OE) cells. The results showed that the level of C3 mRNA in the mouse oviduct was high on day 1 and Day 2, but decreased toa minimum on day 4 of pregnancy, whereas that of pseudo-pregnancy remained relatively stable within the same period. The protein levels of C3 and iC3b specific fragments, α-115 and α-40, respectively in the mouse oviductal luminal fluid were highest on day 3 of pregnancy, when the embryos were expected to be most sensitive to the embryotrophic activity of ETF-3. Estrogen elevated C3 expression in the ovariectomized mouse oviduct and the OE cells. Progesterone suppressed estrogen-induced C3 expression in the mouse oviduct, but had no effect on OE cells. In conclusion, the presence of embryo and steroid hormones regulate the synthesis and secretion of oviductal C3. © 2008 Wiley-Liss, Inc.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/37692en_HK
dc.relation.ispartofMolecular Reproduction and Developmenten_HK
dc.rightsMolecular Reproduction and Development. Copyright © John Wiley & Sons, Inc.en_HK
dc.titleRegulation of complement-3 protein expression in human and mouse oviductsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1040-452X&volume=76&spage=301&epage=308&date=2008&atitle=Regulation+of+complement-3+protein+expression+in+human+and+mouse+oviducts.en_HK
dc.identifier.emailLee, YL:h9316321@hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.authorityLee, YL=rp00308en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/mrd.20955en_HK
dc.identifier.pmid18671285-
dc.identifier.scopuseid_2-s2.0-61449155392en_HK
dc.identifier.hkuros148603en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61449155392&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume76en_HK
dc.identifier.issue3en_HK
dc.identifier.spage301en_HK
dc.identifier.epage308en_HK
dc.identifier.isiWOS:000263202800010-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLee, YL=15033851800en_HK
dc.identifier.scopusauthoridCheong, AWY=24576433900en_HK
dc.identifier.scopusauthoridChow, WN=36852688700en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK

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