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Article: Expression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infection

TitleExpression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infection
Authors
KeywordsChlamydia trachomatis
Ectopic pregnancy
Elafin
Fallopian tube
Secretory leukocyte protease inhibitor
Issue Date2009
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2009, v. 24 n. 3, p. 679-686 How to Cite?
AbstractBACKGROUND: Secretory leukocyte protease inhibitor (SLPI) and elafin are anti-protease and anti-microbial molecules with a role in innate immune defence. They have been demonstrated at multiple mucosal surfaces including those of the female reproductive tract. METHODS AND RESULTS: This study details their expression in human Fallopian tubes (ampullary region) throughout the menstrual cycle (n = 18) and from women with ectopic pregnancy (n = 6), and examined their regulation by infection with Chlamydia trachomatis in an in-vitro model. Quantitative real-time PCR analysis showed that SLPI and elafin were constitutively expressed in the Fallopian tube during the menstrual cycle but were increased in ectopic pregnancy (P < 0.05 versus early-mid luteal phase, P < 0.01 versus all phases, respectively). SLPI and elafin were immunolocalised to the Fallopian tube epithelium in biopsies from non-pregnant women and those with ectopic pregnancy. An in-vitro culture model of C. trachomatis infection of the OE-E6/E7 oviductal epithelial cell line showed that elafin mRNA expression was upregulated in response to chlamydial infection. CONCLUSION: These data suggest that SLPI and elafin have a role in the innate immune defence of the Fallopian tube in infection and ectopic pregnancy. Their role is likely to include regulation of protease activity, wound healing and tissue remodelling. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/60380
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
Funding AgencyGrant Number
Chief Scientist's OfficeCBZ/4/513
Scottish Government Rural and Environment Research and Analysis Directorate (RERAD)
Caledonian Research Foundation
Funding Information:

This research was supported a grant from the Chief Scientist's Office (CBZ/4/513). Nick Wheelhouse and Gary Entrican are funded by the Scottish Government Rural and Environment Research and Analysis Directorate (RERAD). Dr Anne King is supported by a personal research fellowship from the Caledonian Research Foundation.

References

 

DC FieldValueLanguage
dc.contributor.authorKing, AEen_HK
dc.contributor.authorWheelhouse, Nen_HK
dc.contributor.authorCameron, Sen_HK
dc.contributor.authorMcDonald, SEen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorEntrican, Gen_HK
dc.contributor.authorCritchley, HODen_HK
dc.contributor.authorHorne, AWen_HK
dc.date.accessioned2010-05-31T04:09:29Z-
dc.date.available2010-05-31T04:09:29Z-
dc.date.issued2009en_HK
dc.identifier.citationHuman Reproduction, 2009, v. 24 n. 3, p. 679-686en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60380-
dc.description.abstractBACKGROUND: Secretory leukocyte protease inhibitor (SLPI) and elafin are anti-protease and anti-microbial molecules with a role in innate immune defence. They have been demonstrated at multiple mucosal surfaces including those of the female reproductive tract. METHODS AND RESULTS: This study details their expression in human Fallopian tubes (ampullary region) throughout the menstrual cycle (n = 18) and from women with ectopic pregnancy (n = 6), and examined their regulation by infection with Chlamydia trachomatis in an in-vitro model. Quantitative real-time PCR analysis showed that SLPI and elafin were constitutively expressed in the Fallopian tube during the menstrual cycle but were increased in ectopic pregnancy (P < 0.05 versus early-mid luteal phase, P < 0.01 versus all phases, respectively). SLPI and elafin were immunolocalised to the Fallopian tube epithelium in biopsies from non-pregnant women and those with ectopic pregnancy. An in-vitro culture model of C. trachomatis infection of the OE-E6/E7 oviductal epithelial cell line showed that elafin mRNA expression was upregulated in response to chlamydial infection. CONCLUSION: These data suggest that SLPI and elafin have a role in the innate immune defence of the Fallopian tube in infection and ectopic pregnancy. Their role is likely to include regulation of protease activity, wound healing and tissue remodelling. © The Author 2008. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.rightsHuman Reproduction. Copyright © Oxford University Press.en_HK
dc.subjectChlamydia trachomatisen_HK
dc.subjectEctopic pregnancyen_HK
dc.subjectElafinen_HK
dc.subjectFallopian tubeen_HK
dc.subjectSecretory leukocyte protease inhibitoren_HK
dc.titleExpression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infectionen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0268-1161&volume=24&spage=679&epage=86&date=2009&atitle=Expression+of+secretory+leukocyte+protease+inhibitor+and+elafin+in+human+fallopian+tube+and+in+an+in-vitro+model+of+Chlamydia+trachomatis+infection.en_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1093/humrep/den452en_HK
dc.identifier.pmid19095674-
dc.identifier.scopuseid_2-s2.0-61449140184en_HK
dc.identifier.hkuros158113en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-61449140184&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue3en_HK
dc.identifier.spage679en_HK
dc.identifier.epage686en_HK
dc.identifier.isiWOS:000263827800023-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridKing, AE=7403372829en_HK
dc.identifier.scopusauthoridWheelhouse, N=16044431200en_HK
dc.identifier.scopusauthoridCameron, S=7202227877en_HK
dc.identifier.scopusauthoridMcDonald, SE=12780378500en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridEntrican, G=7003339226en_HK
dc.identifier.scopusauthoridCritchley, HOD=7006731536en_HK
dc.identifier.scopusauthoridHorne, AW=8067885000en_HK

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