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Article: Influenza virus in human exhaled breath: An observational study

TitleInfluenza virus in human exhaled breath: An observational study
Authors
KeywordsChemicals And Cas Registry Numbers
Issue Date2008
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2008, v. 3 n. 7 How to Cite?
AbstractBackground: Recent studies suggest that humans exhale fine particles during tidal breathing but little is known of their composition, particularly during infection. Methodology/Principal Findings: We conducted a study of influenza infected patients to characterize influenza virus and particle concentrations in their exhaled breath. Patients presenting with influenza-like-illness, confirmed influenza A or B virus by rapid test, and onset within 3 days were recruited at three clinics in Hong Kong, China. We collected exhaled breath from each subject onto Teflon filters and measured exhaled particle concentrations using an optical particle counter. Filters were analyzed for influenza A and B viruses by quantitative polymerase chain reaction (qPCR). Twelve out of thirteen rapid test positive patients provided exhaled breath filter samples (7 subjects infected with influenza B virus and 5 subjects infected with influenza A virus). We detected influenza virus RNA in the exhaled breath of 4 (33%) subjects-three (60%) of the five patients infected with influenza A virus and one (14%) of the seven infected with influenza B virus. Exhaled influenza virus RNA generation rates ranged from <3.2 to 20 influenza virus RNA particles per minute. Over 87% of particles exhaled were under 1 μm in diameter. Conclusions: These findings regarding influenza virus RNA suggest that influenza virus may be contained in fine particles generated during tidal breathing, and add to the body of literature suggesting that fine particle aerosols may play a role in influenza transmission. © 2008 Fabian et al.
Persistent Identifierhttp://hdl.handle.net/10722/60284
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
US Centers for Disease Control and Prevention1U01CI000439-01
1U01CI000446-01
Research Fund for the Control of Infectious Disease, Food and Health Bureau, Government of the Hong Kong SAR
Area of Excellence Scheme of the Hong Kong University Grants CommitteeAoE/M-12/06
U.S. Federal Aviation Administration (FAA) Office of Aerospace Medicine through the Air Transportation Center of Excellence for Airliner Cabin Environment Research (ACER)04-C-ACE-HU
Funding Information:

This work has received financial support from the US Centers for Disease Control and Prevention (cooperative grants #1U01CI000439-01 and #1U01CI000446-01), the Research Fund for the Control of Infectious Disease, Food and Health Bureau, Government of the Hong Kong SAR, and the Area of Excellence Scheme of the Hong Kong University Grants Committee (grant no. AoE/M-12/06). This work was also supported by the U.S. Federal Aviation Administration (FAA) Office of Aerospace Medicine through the Air Transportation Center of Excellence for Airliner Cabin Environment Research (ACER), Cooperative Agreements 04-C-ACE-HU. Although the FAA and CDC have sponsored this project, they neither endorse nor reject the findings of this research.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorFabian, Pen_HK
dc.contributor.authorMcDevitt, JJen_HK
dc.contributor.authorDeHaan, WHen_HK
dc.contributor.authorFung, ROPen_HK
dc.contributor.authorCowling, BJen_HK
dc.contributor.authorChan, KHen_HK
dc.contributor.authorLeung, GMen_HK
dc.contributor.authorMilton, DKen_HK
dc.date.accessioned2010-05-31T04:07:32Z-
dc.date.available2010-05-31T04:07:32Z-
dc.date.issued2008en_HK
dc.identifier.citationPlos One, 2008, v. 3 n. 7en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60284-
dc.description.abstractBackground: Recent studies suggest that humans exhale fine particles during tidal breathing but little is known of their composition, particularly during infection. Methodology/Principal Findings: We conducted a study of influenza infected patients to characterize influenza virus and particle concentrations in their exhaled breath. Patients presenting with influenza-like-illness, confirmed influenza A or B virus by rapid test, and onset within 3 days were recruited at three clinics in Hong Kong, China. We collected exhaled breath from each subject onto Teflon filters and measured exhaled particle concentrations using an optical particle counter. Filters were analyzed for influenza A and B viruses by quantitative polymerase chain reaction (qPCR). Twelve out of thirteen rapid test positive patients provided exhaled breath filter samples (7 subjects infected with influenza B virus and 5 subjects infected with influenza A virus). We detected influenza virus RNA in the exhaled breath of 4 (33%) subjects-three (60%) of the five patients infected with influenza A virus and one (14%) of the seven infected with influenza B virus. Exhaled influenza virus RNA generation rates ranged from <3.2 to 20 influenza virus RNA particles per minute. Over 87% of particles exhaled were under 1 μm in diameter. Conclusions: These findings regarding influenza virus RNA suggest that influenza virus may be contained in fine particles generated during tidal breathing, and add to the body of literature suggesting that fine particle aerosols may play a role in influenza transmission. © 2008 Fabian et al.en_HK
dc.languageengen_HK
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.subjectChemicals And Cas Registry Numbers-
dc.titleInfluenza virus in human exhaled breath: An observational studyen_HK
dc.typeArticleen_HK
dc.identifier.emailCowling, BJ:bcowling@hku.hken_HK
dc.identifier.emailLeung, GM:gmleung@hku.hken_HK
dc.identifier.authorityCowling, BJ=rp01326en_HK
dc.identifier.authorityLeung, GM=rp00460en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0002691en_HK
dc.identifier.pmid18628983-
dc.identifier.pmcidPMC2442192-
dc.identifier.scopuseid_2-s2.0-49949084652en_HK
dc.identifier.hkuros146898en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-49949084652&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume3en_HK
dc.identifier.issue7en_HK
dc.identifier.isiWOS:000264057200034-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectControl of Pandemic and Inter-pandemic Influenza-
dc.identifier.scopusauthoridFabian, P=24833044400en_HK
dc.identifier.scopusauthoridMcDevitt, JJ=7007043454en_HK
dc.identifier.scopusauthoridDeHaan, WH=24605111300en_HK
dc.identifier.scopusauthoridFung, ROP=24474481300en_HK
dc.identifier.scopusauthoridCowling, BJ=8644765500en_HK
dc.identifier.scopusauthoridChan, KH=7406034307en_HK
dc.identifier.scopusauthoridLeung, GM=7007159841en_HK
dc.identifier.scopusauthoridMilton, DK=35434286800en_HK
dc.identifier.citeulike8028512-

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