File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: The attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes

TitleThe attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genes
Authors
KeywordsMVTT
Vaccine
Vaccinia
Vaccinia Tian Tan
Viral vector
Issue Date2007
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jviromet
Citation
Journal Of Virological Methods, 2007, v. 144 n. 1-2, p. 17-26 How to Cite?
AbstractTo generate a safe vaccinia Tian Tan (VTT)-based vaccine vector, it is necessary to develop a method to attenuate the virus. A modified VTT (MVTT 2-GFP) was constructed by replacing the viral M1L-K2L genes with a GFP gene. In comparison to the parental VTT, MVTT 2-GFP lost its replication capacity in rabbit RK13 and human HeLa cell lines. The life cycle of viral replication was blocked at different stages in these two cell lines as determined by electron microscope examination. MVTT 2-GFP was less virulent than VTT for 100-fold by measuring mouse body weight loss after intranasal viral inoculation and for 340-fold by determining the intracranial LD 50 value in mice. The foreign GFP gene was stable genetically after 10 rounds of passage in Vero cells. Importantly, MVTT 2-GFP elicited both humoral and cell-mediated immune responses to the GFP gene in mice. With two intramuscular inoculations of 10 5 PFU virus, the anti-GFP antibody reciprocal endpoint titer reached over 700 as determined by an ELISA. The number of IFN-γ secreting T cells reached over 350 SFU per million splenocytes against a CD8+ T cell-specific epitope of GFP. Collectively, the removal of the M1L-K2L genes is a useful method to generate an attenuated vaccinia Tian Tan vaccine vector. © 2007 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/60145
ISSN
2015 Impact Factor: 1.508
2015 SCImago Journal Rankings: 0.866
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorZhu, Wen_HK
dc.contributor.authorFang, Qen_HK
dc.contributor.authorZhuang, Ken_HK
dc.contributor.authorWang, Hen_HK
dc.contributor.authorYu, Wen_HK
dc.contributor.authorZhou, Jen_HK
dc.contributor.authorLiu, Len_HK
dc.contributor.authorTien, Pen_HK
dc.contributor.authorZhang, Len_HK
dc.contributor.authorChen, Zen_HK
dc.date.accessioned2010-05-31T04:04:39Z-
dc.date.available2010-05-31T04:04:39Z-
dc.date.issued2007en_HK
dc.identifier.citationJournal Of Virological Methods, 2007, v. 144 n. 1-2, p. 17-26en_HK
dc.identifier.issn0166-0934en_HK
dc.identifier.urihttp://hdl.handle.net/10722/60145-
dc.description.abstractTo generate a safe vaccinia Tian Tan (VTT)-based vaccine vector, it is necessary to develop a method to attenuate the virus. A modified VTT (MVTT 2-GFP) was constructed by replacing the viral M1L-K2L genes with a GFP gene. In comparison to the parental VTT, MVTT 2-GFP lost its replication capacity in rabbit RK13 and human HeLa cell lines. The life cycle of viral replication was blocked at different stages in these two cell lines as determined by electron microscope examination. MVTT 2-GFP was less virulent than VTT for 100-fold by measuring mouse body weight loss after intranasal viral inoculation and for 340-fold by determining the intracranial LD 50 value in mice. The foreign GFP gene was stable genetically after 10 rounds of passage in Vero cells. Importantly, MVTT 2-GFP elicited both humoral and cell-mediated immune responses to the GFP gene in mice. With two intramuscular inoculations of 10 5 PFU virus, the anti-GFP antibody reciprocal endpoint titer reached over 700 as determined by an ELISA. The number of IFN-γ secreting T cells reached over 350 SFU per million splenocytes against a CD8+ T cell-specific epitope of GFP. Collectively, the removal of the M1L-K2L genes is a useful method to generate an attenuated vaccinia Tian Tan vaccine vector. © 2007 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/jvirometen_HK
dc.relation.ispartofJournal of Virological Methodsen_HK
dc.rightsJournal of Virological Methods. Copyright © Elsevier BV.en_HK
dc.subjectMVTTen_HK
dc.subjectVaccineen_HK
dc.subjectVacciniaen_HK
dc.subjectVaccinia Tian Tanen_HK
dc.subjectViral vectoren_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshGene Deletionen_HK
dc.subject.meshGenes, Viralen_HK
dc.subject.meshGenetic Vectorsen_HK
dc.subject.meshGreen Fluorescent Proteins - geneticsen_HK
dc.subject.meshHeLa Cellsen_HK
dc.subject.meshHumansen_HK
dc.subject.meshMiceen_HK
dc.subject.meshMice, Inbred BALB Cen_HK
dc.subject.meshRabbitsen_HK
dc.subject.meshRecombinant Fusion Proteinsen_HK
dc.subject.meshVaccinia virus - genetics - immunology - pathogenicity - physiologyen_HK
dc.subject.meshViral Vaccines - genetics - immunology - metabolismen_HK
dc.subject.meshVirus Replicationen_HK
dc.titleThe attenuation of vaccinia Tian Tan strain by the removal of the viral M1L-K2L genesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0166-0934&volume=144&spage=17&epage=26, Sept. 2007&date=2008&atitle=The+attenuation+of+vaccinia+Tian+Tan+strain+by+the+removal+of+the+viral+M1L-K2L+genesen_HK
dc.identifier.emailLiu, L: liuli71@hkucc.hku.hken_HK
dc.identifier.emailChen, Z: zchenai@hku.hken_HK
dc.identifier.authorityLiu, L=rp00268en_HK
dc.identifier.authorityChen, Z=rp00243en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.jviromet.2007.03.012en_HK
dc.identifier.pmid17459491-
dc.identifier.scopuseid_2-s2.0-34547118382en_HK
dc.identifier.hkuros147740en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34547118382&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume144en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage17en_HK
dc.identifier.epage26en_HK
dc.identifier.isiWOS:000249139100003-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridZhu, W=8377176500en_HK
dc.identifier.scopusauthoridFang, Q=55248545600en_HK
dc.identifier.scopusauthoridZhuang, K=6603626241en_HK
dc.identifier.scopusauthoridWang, H=8724886600en_HK
dc.identifier.scopusauthoridYu, W=50162901500en_HK
dc.identifier.scopusauthoridZhou, J=17344792400en_HK
dc.identifier.scopusauthoridLiu, L=35784425200en_HK
dc.identifier.scopusauthoridTien, P=7006421622en_HK
dc.identifier.scopusauthoridZhang, L=8783285300en_HK
dc.identifier.scopusauthoridChen, Z=35271180800en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats