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Article: A cell penetrating heme oxygenase protein protects heart graft against ischemia/reperfusion injury

TitleA cell penetrating heme oxygenase protein protects heart graft against ischemia/reperfusion injury
Authors
Issue Date2009
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gt
Citation
Gene Therapy, 2009, v. 16 n. 3, p. 320-328 How to Cite?
AbstractIschemia/reperfusion (I/R) injury is an unavoidable barrier that significantly affects outcome of solid organ transplantation. Here, we establish a protein transduction system to extend graft preservation time and to prevent I/R injury in heart transplantation. We generated a recombinant heme oxygenase-1 (HO-1) protein containing a modified protein transduction domain (PTD). PTD could cross cover cell membrane and carry target molecule to parenchymal cells of cold-preserved heart grafts. The newly generated PTD-HO-1 protein localized mainly in subcellular membrane organelle and nucleus after delivery that significantly prolonged cold preservation of heart grafts. This effect was associated with significantly less endothelial cell activation, less neutrophil and macrophage infiltration in PTD-HO-1-transduced heart grafts after reperfusion as compared with controls. In addition, transduction of PTD-HO-1 protein to heart graft significantly suppressed the I/R injury-associated myocardiocyte apoptosis. The infarct areas of heart graft after I/R injury were significantly reduced after PTD-HO-1 protein treatment. We show here for the first time that PTD can maintain its biological activities during cold preservation. Transduction of cell penetrating HO-1 protein significantly prolongs the cold preservation time and protects the graft from the I/R injury. This approach represents a novel method for the improvement of the overall outcome of organ transplantation.
Persistent Identifierhttp://hdl.handle.net/10722/59907
ISSN
2015 Impact Factor: 3.242
2015 SCImago Journal Rankings: 1.519
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, Jen_HK
dc.contributor.authorLau, CKen_HK
dc.contributor.authorObed, Aen_HK
dc.contributor.authorDada, Aen_HK
dc.contributor.authorDoenecke, Aen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorSchlitt, HJen_HK
dc.contributor.authorTsui, TYen_HK
dc.date.accessioned2010-05-31T03:59:52Z-
dc.date.available2010-05-31T03:59:52Z-
dc.date.issued2009en_HK
dc.identifier.citationGene Therapy, 2009, v. 16 n. 3, p. 320-328en_HK
dc.identifier.issn0969-7128en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59907-
dc.description.abstractIschemia/reperfusion (I/R) injury is an unavoidable barrier that significantly affects outcome of solid organ transplantation. Here, we establish a protein transduction system to extend graft preservation time and to prevent I/R injury in heart transplantation. We generated a recombinant heme oxygenase-1 (HO-1) protein containing a modified protein transduction domain (PTD). PTD could cross cover cell membrane and carry target molecule to parenchymal cells of cold-preserved heart grafts. The newly generated PTD-HO-1 protein localized mainly in subcellular membrane organelle and nucleus after delivery that significantly prolonged cold preservation of heart grafts. This effect was associated with significantly less endothelial cell activation, less neutrophil and macrophage infiltration in PTD-HO-1-transduced heart grafts after reperfusion as compared with controls. In addition, transduction of PTD-HO-1 protein to heart graft significantly suppressed the I/R injury-associated myocardiocyte apoptosis. The infarct areas of heart graft after I/R injury were significantly reduced after PTD-HO-1 protein treatment. We show here for the first time that PTD can maintain its biological activities during cold preservation. Transduction of cell penetrating HO-1 protein significantly prolongs the cold preservation time and protects the graft from the I/R injury. This approach represents a novel method for the improvement of the overall outcome of organ transplantation.en_HK
dc.languageengen_HK
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/gten_HK
dc.relation.ispartofGene Therapyen_HK
dc.titleA cell penetrating heme oxygenase protein protects heart graft against ischemia/reperfusion injuryen_HK
dc.typeArticleen_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1038/gt.2008.162en_HK
dc.identifier.pmid18987674-
dc.identifier.scopuseid_2-s2.0-62549137837en_HK
dc.identifier.hkuros155667en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-62549137837&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume16en_HK
dc.identifier.issue3en_HK
dc.identifier.spage320en_HK
dc.identifier.epage328en_HK
dc.identifier.isiWOS:000264115900003-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMa, J=7406201578en_HK
dc.identifier.scopusauthoridLau, CK=7401968442en_HK
dc.identifier.scopusauthoridObed, A=23009911000en_HK
dc.identifier.scopusauthoridDada, A=6602121067en_HK
dc.identifier.scopusauthoridDoenecke, A=8098869700en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridSchlitt, HJ=7005572464en_HK
dc.identifier.scopusauthoridTsui, TY=7006622455en_HK
dc.identifier.citeulike3484606-

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