File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Fc-dependent expression of CD137 on human NK cells: Insights into "agonistic" effects of anti-CD137 monoclonal antibodies

TitleFc-dependent expression of CD137 on human NK cells: Insights into "agonistic" effects of anti-CD137 monoclonal antibodies
Authors
Issue Date2008
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2008, v. 112 n. 3, p. 699-707 How to Cite?
AbstractCD137 (4-1 BB) is a costimulatory molecule that can be manipulated for the treatment of cancer and autoimmune disease. Although it is known that agonistic antibodies (mAbs) against CD137 enhance the rejection of murine tumors in a natural killer (NK) cell- and T cell-dependent fashion, the mechanism for NK dependence is poorly understood. In this study, we evaluated the ability of 2 different glycoforms of a chimerized antihuman CD137 mAb, an aglycosylated (GA) and a low fucose form (GG), to react with human NK cells. Both mAbs bound similarly to CD137 and partially blocked the interaction between CD137 and CD137 ligand. However, unlike GA mAb, immobilized GG mAb activated NK cells and enhanced CD137 expression. These effects were seemingly dependent on Fc interaction with putative Fc receptors on the NK-cell surface, as only the immobilized Fc-fragment of GG was required for CD137 expression. Furthermore, CD137 expression could be enhanced with antibodies directed against non-CD137 epitopes, and the expression levels directly correlated with patterns of Fc-glycosylation recognized to improve Fc interaction with Fcγ receptors. Our data suggest that CD137 can be enhanced on NK cells in an Fc-dependent fashion and that expression correlates with phenotypic and functional parameters of activation. © 2008 by The American Society of Hematology.
Persistent Identifierhttp://hdl.handle.net/10722/59850
ISSN
2015 Impact Factor: 11.841
2015 SCImago Journal Rankings: 6.395
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLin, Wen_HK
dc.contributor.authorVoskens, CJen_HK
dc.contributor.authorZhang, Xen_HK
dc.contributor.authorSchindler, DGen_HK
dc.contributor.authorWood, Aen_HK
dc.contributor.authorBurch, Een_HK
dc.contributor.authorWei, Yen_HK
dc.contributor.authorChen, Len_HK
dc.contributor.authorTian, Gen_HK
dc.contributor.authorTamada, Ken_HK
dc.contributor.authorWang, LXen_HK
dc.contributor.authorSchulze, DHen_HK
dc.contributor.authorMann, Den_HK
dc.contributor.authorStrome, SEen_HK
dc.date.accessioned2010-05-31T03:58:45Z-
dc.date.available2010-05-31T03:58:45Z-
dc.date.issued2008en_HK
dc.identifier.citationBlood, 2008, v. 112 n. 3, p. 699-707en_HK
dc.identifier.issn0006-4971en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59850-
dc.description.abstractCD137 (4-1 BB) is a costimulatory molecule that can be manipulated for the treatment of cancer and autoimmune disease. Although it is known that agonistic antibodies (mAbs) against CD137 enhance the rejection of murine tumors in a natural killer (NK) cell- and T cell-dependent fashion, the mechanism for NK dependence is poorly understood. In this study, we evaluated the ability of 2 different glycoforms of a chimerized antihuman CD137 mAb, an aglycosylated (GA) and a low fucose form (GG), to react with human NK cells. Both mAbs bound similarly to CD137 and partially blocked the interaction between CD137 and CD137 ligand. However, unlike GA mAb, immobilized GG mAb activated NK cells and enhanced CD137 expression. These effects were seemingly dependent on Fc interaction with putative Fc receptors on the NK-cell surface, as only the immobilized Fc-fragment of GG was required for CD137 expression. Furthermore, CD137 expression could be enhanced with antibodies directed against non-CD137 epitopes, and the expression levels directly correlated with patterns of Fc-glycosylation recognized to improve Fc interaction with Fcγ receptors. Our data suggest that CD137 can be enhanced on NK cells in an Fc-dependent fashion and that expression correlates with phenotypic and functional parameters of activation. © 2008 by The American Society of Hematology.en_HK
dc.languageengen_HK
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/en_HK
dc.relation.ispartofBlooden_HK
dc.titleFc-dependent expression of CD137 on human NK cells: Insights into "agonistic" effects of anti-CD137 monoclonal antibodiesen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0006-4971&volume=112&issue=3&spage=699&epage=707&date=2008&atitle=Fc+Dependent+Expression+of+CD137+on+Human+NK+Cells:+Insights+into+“Agonistic+Effects+of+Anti-CD137+Monoclonal+Antibodiesen_HK
dc.identifier.emailTian, G: gltian@hku.hken_HK
dc.identifier.authorityTian, G=rp00789en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1182/blood-2007-11-122465en_HK
dc.identifier.scopuseid_2-s2.0-50949132226en_HK
dc.identifier.hkuros163575en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-50949132226&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume112en_HK
dc.identifier.issue3en_HK
dc.identifier.spage699en_HK
dc.identifier.epage707en_HK
dc.identifier.isiWOS:000258257900037-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLin, W=36068291300en_HK
dc.identifier.scopusauthoridVoskens, CJ=24765663400en_HK
dc.identifier.scopusauthoridZhang, X=35591044500en_HK
dc.identifier.scopusauthoridSchindler, DG=7102770244en_HK
dc.identifier.scopusauthoridWood, A=7401883108en_HK
dc.identifier.scopusauthoridBurch, E=35589613000en_HK
dc.identifier.scopusauthoridWei, Y=7404094369en_HK
dc.identifier.scopusauthoridChen, L=35323760200en_HK
dc.identifier.scopusauthoridTian, G=25621549400en_HK
dc.identifier.scopusauthoridTamada, K=7103030557en_HK
dc.identifier.scopusauthoridWang, LX=35231383100en_HK
dc.identifier.scopusauthoridSchulze, DH=7102867976en_HK
dc.identifier.scopusauthoridMann, D=35196578800en_HK
dc.identifier.scopusauthoridStrome, SE=7004882323en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats