File Download
There are no files associated with this item.
Links for fulltext
(May Require Subscription)
- Publisher Website: 10.1016/j.gene.2009.02.007
- Scopus: eid_2-s2.0-63649100553
- PMID: 19236911
- WOS: WOS:000265725300006
- Find via
Supplementary
- Citations:
- Appears in Collections:
Article: Structure and promoter characterization of aldo-keto reductase family 1 B10 gene
Title | Structure and promoter characterization of aldo-keto reductase family 1 B10 gene | ||||||
---|---|---|---|---|---|---|---|
Authors | |||||||
Keywords | AKR1B10 Aldose reductase-like-1 ARL-1 Gene structure Promoter | ||||||
Issue Date | 2009 | ||||||
Publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene | ||||||
Citation | Gene, 2009, v. 437 n. 1-2, p. 39-44 How to Cite? | ||||||
Abstract | Aldo-keto reductase family 1 member B10 (AKR1B10) is overexpressed in human hepatocellular carcinoma, lung squamous carcinoma, and lung adenocarcinoma in smokers. Our recent studies have showed that AKR1B10 plays a critical role in the growth and proliferation of cancer cells by detoxifying reactive carbonyls and regulating fatty acid biosynthesis. However, little is known about the regulatory mechanisms of AKR1B10 expression. In this study, we determined the structure of AKR1B10 gene and characterized its promoter. The results demonstrated that AKR1B10 consists of 10 exons and 9 introns, stretching approximately 13.8 kb. A 5′-RACE study determined the transcriptional start site of AKR1B10 at 320 bp upstream of the ATG translational start codon. A TATA-like (TAATAA) and a CAAT box are present from - 145 to - 140 bp and - 193 to - 190 bp upstream of the transcriptional start site, respectively. Motif analysis recognized multiple putative oncogenic and tumor suppressor protein binding sites in the AKR1B10 promoter, including c-Ets-1, C/EBP, AP-1, and p53, but osmolytic response elements were not found. A - 4091 bp of the 5′-flanking fragment of the AKR1B10 gene was capable of driving GFP and luciferase reporter gene expression in HepG2 cells derived from human hepatocellular carcinoma; progressive 5′-deletions revealed that a - 255 bp fragment possesses full promoter activity. © 2008 Elsevier B.V. All rights reserved. | ||||||
Persistent Identifier | http://hdl.handle.net/10722/59679 | ||||||
ISSN | 2023 Impact Factor: 2.6 2023 SCImago Journal Rankings: 0.725 | ||||||
ISI Accession Number ID |
Funding Information: This work was supported in part by American Cancer Society grant (RSG-04-031-01-CCE) and National Cancer institute (CA122327 and CA122622). | ||||||
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Liu, Z | en_HK |
dc.contributor.author | Zhong, L | en_HK |
dc.contributor.author | Krishack, PA | en_HK |
dc.contributor.author | Robbins, S | en_HK |
dc.contributor.author | Cao, JX | en_HK |
dc.contributor.author | Zhao, Y | en_HK |
dc.contributor.author | Chung, S | en_HK |
dc.contributor.author | Cao, D | en_HK |
dc.date.accessioned | 2010-05-31T03:55:06Z | - |
dc.date.available | 2010-05-31T03:55:06Z | - |
dc.date.issued | 2009 | en_HK |
dc.identifier.citation | Gene, 2009, v. 437 n. 1-2, p. 39-44 | en_HK |
dc.identifier.issn | 0378-1119 | en_HK |
dc.identifier.uri | http://hdl.handle.net/10722/59679 | - |
dc.description.abstract | Aldo-keto reductase family 1 member B10 (AKR1B10) is overexpressed in human hepatocellular carcinoma, lung squamous carcinoma, and lung adenocarcinoma in smokers. Our recent studies have showed that AKR1B10 plays a critical role in the growth and proliferation of cancer cells by detoxifying reactive carbonyls and regulating fatty acid biosynthesis. However, little is known about the regulatory mechanisms of AKR1B10 expression. In this study, we determined the structure of AKR1B10 gene and characterized its promoter. The results demonstrated that AKR1B10 consists of 10 exons and 9 introns, stretching approximately 13.8 kb. A 5′-RACE study determined the transcriptional start site of AKR1B10 at 320 bp upstream of the ATG translational start codon. A TATA-like (TAATAA) and a CAAT box are present from - 145 to - 140 bp and - 193 to - 190 bp upstream of the transcriptional start site, respectively. Motif analysis recognized multiple putative oncogenic and tumor suppressor protein binding sites in the AKR1B10 promoter, including c-Ets-1, C/EBP, AP-1, and p53, but osmolytic response elements were not found. A - 4091 bp of the 5′-flanking fragment of the AKR1B10 gene was capable of driving GFP and luciferase reporter gene expression in HepG2 cells derived from human hepatocellular carcinoma; progressive 5′-deletions revealed that a - 255 bp fragment possesses full promoter activity. © 2008 Elsevier B.V. All rights reserved. | en_HK |
dc.language | eng | en_HK |
dc.publisher | Elsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene | en_HK |
dc.relation.ispartof | Gene | en_HK |
dc.rights | Gene. Copyright © Elsevier BV. | en_HK |
dc.subject | AKR1B10 | en_HK |
dc.subject | Aldose reductase-like-1 | en_HK |
dc.subject | ARL-1 | en_HK |
dc.subject | Gene structure | en_HK |
dc.subject | Promoter | en_HK |
dc.title | Structure and promoter characterization of aldo-keto reductase family 1 B10 gene | en_HK |
dc.type | Article | en_HK |
dc.identifier.openurl | http://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-1119&volume=437&spage=39&epage=44&date=2009&atitle=Structure+And+Promoter+Characterization+Of+Aldo-keto+Reductase+Family+1+B10+Gene | en_HK |
dc.identifier.email | Chung, S: smchung@hkucc.hku.hk | en_HK |
dc.identifier.authority | Chung, S=rp00376 | en_HK |
dc.description.nature | link_to_subscribed_fulltext | - |
dc.identifier.doi | 10.1016/j.gene.2009.02.007 | en_HK |
dc.identifier.pmid | 19236911 | - |
dc.identifier.scopus | eid_2-s2.0-63649100553 | en_HK |
dc.identifier.hkuros | 167843 | en_HK |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-63649100553&selection=ref&src=s&origin=recordpage | en_HK |
dc.identifier.volume | 437 | en_HK |
dc.identifier.issue | 1-2 | en_HK |
dc.identifier.spage | 39 | en_HK |
dc.identifier.epage | 44 | en_HK |
dc.identifier.isi | WOS:000265725300006 | - |
dc.publisher.place | Netherlands | en_HK |
dc.identifier.scopusauthorid | Liu, Z=14058396700 | en_HK |
dc.identifier.scopusauthorid | Zhong, L=26323899800 | en_HK |
dc.identifier.scopusauthorid | Krishack, PA=13807702500 | en_HK |
dc.identifier.scopusauthorid | Robbins, S=17136355600 | en_HK |
dc.identifier.scopusauthorid | Cao, JX=26323522800 | en_HK |
dc.identifier.scopusauthorid | Zhao, Y=35727384600 | en_HK |
dc.identifier.scopusauthorid | Chung, S=14120761600 | en_HK |
dc.identifier.scopusauthorid | Cao, D=7202125033 | en_HK |
dc.identifier.issnl | 0378-1119 | - |