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Article: Structure and promoter characterization of aldo-keto reductase family 1 B10 gene

TitleStructure and promoter characterization of aldo-keto reductase family 1 B10 gene
Authors
KeywordsAKR1B10
Aldose reductase-like-1
ARL-1
Gene structure
Promoter
Issue Date2009
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/gene
Citation
Gene, 2009, v. 437 n. 1-2, p. 39-44 How to Cite?
AbstractAldo-keto reductase family 1 member B10 (AKR1B10) is overexpressed in human hepatocellular carcinoma, lung squamous carcinoma, and lung adenocarcinoma in smokers. Our recent studies have showed that AKR1B10 plays a critical role in the growth and proliferation of cancer cells by detoxifying reactive carbonyls and regulating fatty acid biosynthesis. However, little is known about the regulatory mechanisms of AKR1B10 expression. In this study, we determined the structure of AKR1B10 gene and characterized its promoter. The results demonstrated that AKR1B10 consists of 10 exons and 9 introns, stretching approximately 13.8 kb. A 5′-RACE study determined the transcriptional start site of AKR1B10 at 320 bp upstream of the ATG translational start codon. A TATA-like (TAATAA) and a CAAT box are present from - 145 to - 140 bp and - 193 to - 190 bp upstream of the transcriptional start site, respectively. Motif analysis recognized multiple putative oncogenic and tumor suppressor protein binding sites in the AKR1B10 promoter, including c-Ets-1, C/EBP, AP-1, and p53, but osmolytic response elements were not found. A - 4091 bp of the 5′-flanking fragment of the AKR1B10 gene was capable of driving GFP and luciferase reporter gene expression in HepG2 cells derived from human hepatocellular carcinoma; progressive 5′-deletions revealed that a - 255 bp fragment possesses full promoter activity. © 2008 Elsevier B.V. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/59679
ISSN
2015 Impact Factor: 2.319
2015 SCImago Journal Rankings: 1.043
ISI Accession Number ID
Funding AgencyGrant Number
American Cancer SocietyRSG-04-031-01-CCE
National Cancer instituteCA122327
CA122622
Funding Information:

This work was supported in part by American Cancer Society grant (RSG-04-031-01-CCE) and National Cancer institute (CA122327 and CA122622).

References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Zen_HK
dc.contributor.authorZhong, Len_HK
dc.contributor.authorKrishack, PAen_HK
dc.contributor.authorRobbins, Sen_HK
dc.contributor.authorCao, JXen_HK
dc.contributor.authorZhao, Yen_HK
dc.contributor.authorChung, Sen_HK
dc.contributor.authorCao, Den_HK
dc.date.accessioned2010-05-31T03:55:06Z-
dc.date.available2010-05-31T03:55:06Z-
dc.date.issued2009en_HK
dc.identifier.citationGene, 2009, v. 437 n. 1-2, p. 39-44en_HK
dc.identifier.issn0378-1119en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59679-
dc.description.abstractAldo-keto reductase family 1 member B10 (AKR1B10) is overexpressed in human hepatocellular carcinoma, lung squamous carcinoma, and lung adenocarcinoma in smokers. Our recent studies have showed that AKR1B10 plays a critical role in the growth and proliferation of cancer cells by detoxifying reactive carbonyls and regulating fatty acid biosynthesis. However, little is known about the regulatory mechanisms of AKR1B10 expression. In this study, we determined the structure of AKR1B10 gene and characterized its promoter. The results demonstrated that AKR1B10 consists of 10 exons and 9 introns, stretching approximately 13.8 kb. A 5′-RACE study determined the transcriptional start site of AKR1B10 at 320 bp upstream of the ATG translational start codon. A TATA-like (TAATAA) and a CAAT box are present from - 145 to - 140 bp and - 193 to - 190 bp upstream of the transcriptional start site, respectively. Motif analysis recognized multiple putative oncogenic and tumor suppressor protein binding sites in the AKR1B10 promoter, including c-Ets-1, C/EBP, AP-1, and p53, but osmolytic response elements were not found. A - 4091 bp of the 5′-flanking fragment of the AKR1B10 gene was capable of driving GFP and luciferase reporter gene expression in HepG2 cells derived from human hepatocellular carcinoma; progressive 5′-deletions revealed that a - 255 bp fragment possesses full promoter activity. © 2008 Elsevier B.V. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/geneen_HK
dc.relation.ispartofGeneen_HK
dc.rightsGene. Copyright © Elsevier BV.en_HK
dc.subjectAKR1B10en_HK
dc.subjectAldose reductase-like-1en_HK
dc.subjectARL-1en_HK
dc.subjectGene structureen_HK
dc.subjectPromoteren_HK
dc.titleStructure and promoter characterization of aldo-keto reductase family 1 B10 geneen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0378-1119&volume=437&spage=39&epage=44&date=2009&atitle=Structure+And+Promoter+Characterization+Of+Aldo-keto+Reductase+Family+1+B10+Geneen_HK
dc.identifier.emailChung, S: smchung@hkucc.hku.hken_HK
dc.identifier.authorityChung, S=rp00376en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.gene.2009.02.007en_HK
dc.identifier.pmid19236911-
dc.identifier.scopuseid_2-s2.0-63649100553en_HK
dc.identifier.hkuros167843en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-63649100553&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume437en_HK
dc.identifier.issue1-2en_HK
dc.identifier.spage39en_HK
dc.identifier.epage44en_HK
dc.identifier.isiWOS:000265725300006-
dc.publisher.placeNetherlandsen_HK
dc.identifier.scopusauthoridLiu, Z=14058396700en_HK
dc.identifier.scopusauthoridZhong, L=26323899800en_HK
dc.identifier.scopusauthoridKrishack, PA=13807702500en_HK
dc.identifier.scopusauthoridRobbins, S=17136355600en_HK
dc.identifier.scopusauthoridCao, JX=26323522800en_HK
dc.identifier.scopusauthoridZhao, Y=35727384600en_HK
dc.identifier.scopusauthoridChung, S=14120761600en_HK
dc.identifier.scopusauthoridCao, D=7202125033en_HK

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