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Article: Differential expression of GABA receptor subunit messenger ribonucleic acids and immunoreactivity in the rat neostriatum during postnatal development

TitleDifferential expression of GABA receptor subunit messenger ribonucleic acids and immunoreactivity in the rat neostriatum during postnatal development
Authors
KeywordsBasal ganglia
Choline acetyltransferase-immunoreactive neurons
Gene expression
Immunofluorescence
Neostriatum
Parvalbumin-immunoreactive neurons
Reverse transcriptase-polymerase chain reaction
Striatal neurons
Issue Date2008
PublisherS Karger AG. The Journal's web site is located at http://www.karger.com/nba
Citation
Neuroembryology And Aging, 2008, v. 5 n. 3, p. 100-115 How to Cite?
Abstractγ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter in the neostriatum. Functions of GABA are mediated by GABAA and GABAB receptors in the neostriatum. In order to investigate the developmental expression of GABA receptor subunits (GABAAα 1, GABAAα3 and GABAAα 6; GABAAβ2 and GABAAβ 3, and GABABR1 and GABABR 2) in the rat neostriatum, reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence were performed. Tissues were obtained from rats on postnatal day (PND) 1, PND 7 and PND 14, and from adult rats. RT-PCR indicated that GABAAα1 and GABA Aα6 mRNA levels were low, but that GABA Aβ2 and GABAAβ3 mRNAs levels were high during the early postnatal period. Immunofluorescence revealed that GABAAα1 immunoreactivity was only observed in striatal interneurons in PND 14 and in adult rats. Immunoreactivity for GABAAα6 was only observed in PND 14 and adult animals. GABABR1 immunoreactivity was found to be expressed by perikarya of striatal neurons at all ages examined. In contrast, GABABR2 immunoreactivity was mainly observed in choline acetyltransferase-positive striatal interneurons in PND 14 and adult rats. The present results indicate that there are differential patterns of developmental expression of GABA receptor subunits in the neostriatum, with important implications for the development of GABA systems in rat basal ganglia. Copyright © 2008 S. Karger AG.
Persistent Identifierhttp://hdl.handle.net/10722/59674
ISSN
2011 SCImago Journal Rankings: 0.130
References

 

DC FieldValueLanguage
dc.contributor.authorLau, WKen_HK
dc.contributor.authorZhang, HQen_HK
dc.contributor.authorChan, YSen_HK
dc.contributor.authorYung, KKLen_HK
dc.date.accessioned2010-05-31T03:55:00Z-
dc.date.available2010-05-31T03:55:00Z-
dc.date.issued2008en_HK
dc.identifier.citationNeuroembryology And Aging, 2008, v. 5 n. 3, p. 100-115en_HK
dc.identifier.issn1661-3406en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59674-
dc.description.abstractγ-Aminobutyric acid (GABA) is a major inhibitory neurotransmitter in the neostriatum. Functions of GABA are mediated by GABAA and GABAB receptors in the neostriatum. In order to investigate the developmental expression of GABA receptor subunits (GABAAα 1, GABAAα3 and GABAAα 6; GABAAβ2 and GABAAβ 3, and GABABR1 and GABABR 2) in the rat neostriatum, reverse transcriptase-polymerase chain reaction (RT-PCR) and immunofluorescence were performed. Tissues were obtained from rats on postnatal day (PND) 1, PND 7 and PND 14, and from adult rats. RT-PCR indicated that GABAAα1 and GABA Aα6 mRNA levels were low, but that GABA Aβ2 and GABAAβ3 mRNAs levels were high during the early postnatal period. Immunofluorescence revealed that GABAAα1 immunoreactivity was only observed in striatal interneurons in PND 14 and in adult rats. Immunoreactivity for GABAAα6 was only observed in PND 14 and adult animals. GABABR1 immunoreactivity was found to be expressed by perikarya of striatal neurons at all ages examined. In contrast, GABABR2 immunoreactivity was mainly observed in choline acetyltransferase-positive striatal interneurons in PND 14 and adult rats. The present results indicate that there are differential patterns of developmental expression of GABA receptor subunits in the neostriatum, with important implications for the development of GABA systems in rat basal ganglia. Copyright © 2008 S. Karger AG.en_HK
dc.languageengen_HK
dc.publisherS Karger AG. The Journal's web site is located at http://www.karger.com/nbaen_HK
dc.relation.ispartofNeuroembryology and Agingen_HK
dc.rightsNeuroembryology and Aging. Copyright © S Karger AG.en_HK
dc.subjectBasal gangliaen_HK
dc.subjectCholine acetyltransferase-immunoreactive neuronsen_HK
dc.subjectGene expressionen_HK
dc.subjectImmunofluorescenceen_HK
dc.subjectNeostriatumen_HK
dc.subjectParvalbumin-immunoreactive neuronsen_HK
dc.subjectReverse transcriptase-polymerase chain reactionen_HK
dc.subjectStriatal neuronsen_HK
dc.titleDifferential expression of GABA receptor subunit messenger ribonucleic acids and immunoreactivity in the rat neostriatum during postnatal developmenten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1661-3406&volume=5&spage=100&epage=115&date=2008&atitle=Differential+expression+of+GABA+receptor+subunits+messenger+ribonucleic+acids+and+immunoreactivity+in+the+rat+neostriatum+during+postnatal+developmenten_HK
dc.identifier.emailChan, YS: yschan@hkucc.hku.hken_HK
dc.identifier.authorityChan, YS=rp00318en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1159/000178808en_HK
dc.identifier.scopuseid_2-s2.0-57749170980en_HK
dc.identifier.hkuros164093en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-57749170980&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume5en_HK
dc.identifier.issue3en_HK
dc.identifier.spage100en_HK
dc.identifier.epage115en_HK
dc.publisher.placeSwitzerlanden_HK
dc.identifier.scopusauthoridLau, WK=7402933189en_HK
dc.identifier.scopusauthoridZhang, HQ=7409196725en_HK
dc.identifier.scopusauthoridChan, YS=7403676627en_HK
dc.identifier.scopusauthoridYung, KKL=13605496000en_HK

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