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Article: Mechanism-based small molecule probes for labeling CD38 on live cells

TitleMechanism-based small molecule probes for labeling CD38 on live cells
Authors
KeywordsAntibody labeling
Cell activity
Controlled study
Human
Human cell
Molecular mechanics
Molecular probe
Mutant
Polyacrylamide gel electrophoresis
Protein expression
Protein synthesis
Ultraviolet radiation
Wild type
Issue Date2009
PublisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.html
Citation
Journal Of The American Chemical Society, 2009, v. 131 n. 5, p. 1658-1659 How to Cite?
AbstractCD38 is a type II transmembrane glycoprotein with multiple functions. It acts as an ecto-enzyme as well as a receptor. The enzymatic activity catalyzes the formation of two potent Ca 2+ releasing agents: cyclic adenosine diphosphate ribose (cADPR) from nicotinamide adenine dinucleotide (NAD) and nicotinic acid adenine dinucleotide phosphate (NAADP) from NAD phosphate (NADP). The receptor function of CD38 leads to the phosphorylation of intracellular signaling proteins and the up-regulation of cytokine production in immune cells. These two functions of CD38 underlie its involvement in various biological processes, such as hormone secretion, immune cell differentiation, and immune responses. Clinically, CD38 is used as a negative prognosis marker for chronic lymphatic leukemia (CLL). However, a clear molecular understanding of CD38's role in physiology and pathology is still lacking. To facilitate the study of CD38 at cellular and molecular levels, here we report a mechanism-based method for fluorescently labeling CD38 on live cells. This labeling method does not interfere with the receptor function of CD38 and the downstream signaling. The labeling method is thus a useful tool to study the receptor function of CD38 in live cells. In addition, since the mechanism-based labeling also inhibits the enzymatic activity of CD38, it should be useful for dissecting the receptor function of CD38 without interference from its enzyme function in complicated biological processes. Copyright © 2009 American Chemical Society.
Persistent Identifierhttp://hdl.handle.net/10722/59670
ISSN
2015 Impact Factor: 13.038
2015 SCImago Journal Rankings: 7.123
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
NIH USPHS
NYSTEM
Funding Information:

This work is partly supported by The Camille and Henry Dreyfus New Faculty Award Program (H.L.) and grants from the NIH USPHS (A.Y.) and NYSTEM (A.Y.).

References

 

DC FieldValueLanguage
dc.contributor.authorJiang, Hen_HK
dc.contributor.authorCongleton, Jen_HK
dc.contributor.authorLiu, Qen_HK
dc.contributor.authorMerchant, Pen_HK
dc.contributor.authorMalavasi, Fen_HK
dc.contributor.authorLee, HCen_HK
dc.contributor.authorHao, Qen_HK
dc.contributor.authorYen, Aen_HK
dc.contributor.authorLin, Hen_HK
dc.date.accessioned2010-05-31T03:54:55Z-
dc.date.available2010-05-31T03:54:55Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of The American Chemical Society, 2009, v. 131 n. 5, p. 1658-1659en_HK
dc.identifier.issn0002-7863en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59670-
dc.description.abstractCD38 is a type II transmembrane glycoprotein with multiple functions. It acts as an ecto-enzyme as well as a receptor. The enzymatic activity catalyzes the formation of two potent Ca 2+ releasing agents: cyclic adenosine diphosphate ribose (cADPR) from nicotinamide adenine dinucleotide (NAD) and nicotinic acid adenine dinucleotide phosphate (NAADP) from NAD phosphate (NADP). The receptor function of CD38 leads to the phosphorylation of intracellular signaling proteins and the up-regulation of cytokine production in immune cells. These two functions of CD38 underlie its involvement in various biological processes, such as hormone secretion, immune cell differentiation, and immune responses. Clinically, CD38 is used as a negative prognosis marker for chronic lymphatic leukemia (CLL). However, a clear molecular understanding of CD38's role in physiology and pathology is still lacking. To facilitate the study of CD38 at cellular and molecular levels, here we report a mechanism-based method for fluorescently labeling CD38 on live cells. This labeling method does not interfere with the receptor function of CD38 and the downstream signaling. The labeling method is thus a useful tool to study the receptor function of CD38 in live cells. In addition, since the mechanism-based labeling also inhibits the enzymatic activity of CD38, it should be useful for dissecting the receptor function of CD38 without interference from its enzyme function in complicated biological processes. Copyright © 2009 American Chemical Society.en_HK
dc.languageengen_HK
dc.publisherAmerican Chemical Society. The Journal's web site is located at http://pubs.acs.org/journals/jacsat/index.htmlen_HK
dc.relation.ispartofJournal of the American Chemical Societyen_HK
dc.subjectAntibody labelingen_HK
dc.subjectCell activityen_HK
dc.subjectControlled studyen_HK
dc.subjectHumanen_HK
dc.subjectHuman cellen_HK
dc.subjectMolecular mechanicsen_HK
dc.subjectMolecular probeen_HK
dc.subjectMutanten_HK
dc.subjectPolyacrylamide gel electrophoresisen_HK
dc.subjectProtein expressionen_HK
dc.subjectProtein synthesisen_HK
dc.subjectUltraviolet radiationen_HK
dc.subjectWild typeen_HK
dc.titleMechanism-based small molecule probes for labeling CD38 on live cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLee, HC: leehc@hku.hken_HK
dc.identifier.emailHao, Q: qhao@hku.hken_HK
dc.identifier.authorityLee, HC=rp00545en_HK
dc.identifier.authorityHao, Q=rp01332en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1021/ja808387gen_US
dc.identifier.pmid19191692-
dc.identifier.pmcidPMC2644825-
dc.identifier.scopuseid_2-s2.0-67849110365en_HK
dc.identifier.hkuros154373en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67849110365&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume131en_HK
dc.identifier.issue5en_HK
dc.identifier.spage1658en_HK
dc.identifier.epage1659en_HK
dc.identifier.isiWOS:000264792100018-
dc.publisher.placeUnited Statesen_HK
dc.identifier.f10001157228-
dc.identifier.scopusauthoridJiang, H=27171339900en_HK
dc.identifier.scopusauthoridCongleton, J=36713168800en_HK
dc.identifier.scopusauthoridLiu, Q=35215401600en_HK
dc.identifier.scopusauthoridMerchant, P=36714135000en_HK
dc.identifier.scopusauthoridMalavasi, F=7004453948en_HK
dc.identifier.scopusauthoridLee, HC=26642959100en_HK
dc.identifier.scopusauthoridHao, Q=7102508868en_HK
dc.identifier.scopusauthoridYen, A=7102918526en_HK
dc.identifier.scopusauthoridLin, H=8686527600en_HK

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