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Article: Hypertension and the absence of EDHF-mediated responses favour endothelium-dependent contractions in renal arteries of the rat

TitleHypertension and the absence of EDHF-mediated responses favour endothelium-dependent contractions in renal arteries of the rat
Authors
KeywordsACh
COX
EP-1 receptors
NOS
TP receptors
Issue Date2008
PublisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1
Citation
British Journal Of Pharmacology, 2008, v. 155 n. 2, p. 217-226 How to Cite?
AbstractBackground and purpose: Experiments were designed to determine the modulation by nitric oxide (NO) and endothelium-dependent hyperpolarizations (EDHF-mediated responses) of endothelium-dependent contractions in renal arteries of normotensive and hypertensive rats. Experimental approach: Rings, with or without endothelium, of renal arteries of 8-month-old Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were suspended in myographs for isometric force recording. Key results: ACh evoked relaxations in preparations contracted with phenylephrine. L-NAME (inhibitor of NOS) attenuated (WKY) or abolished (SHR) these relaxations. TRAM-34 plus UCL 1684 (inhibitors of EDHF-mediated responses) did not decrease the relaxation, except in rings of WKY when L-NAME was also present. High concentrations of ACh caused a secondary increase in tension, augmented in rings of WKY by L-NAME or TRAM-34 plus UCL 1684. The increase in tension was prevented by indomethacin. Under baseline tension, ACh induced endothelium-dependent contractions, prevented by indomethacin (COX inhibitor) or terutroban (TP receptor antagonist). The calculated endothelium-dependent contractions were larger in rings of SHR compared with those of WKY. In preparations of SHR, the contractions were augmented by L-NAME in the presence of SC19220 (EP-1 receptor antagonist). In arteries of WKY, the endothelium-dependent contractions were augmented by TRAM-34 plus UCL 1684. The responses were reduced by SC19220. Conclusions and implications: In the renal artery of the rat, EDCF-mediated contractions are augmented by hypertension. The endothelium-dependent contractions are facilitated by NOS inhibition (in the presence of an EP-1 receptor antagonist) and by the withdrawal of EDHF-mediated responses. © 2008 Macmillan Publishers Limited All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/59556
ISSN
2015 Impact Factor: 5.259
2015 SCImago Journal Rankings: 2.368
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
FRM (Fondation pour la Recherche Medicale, France)SPE20050303429
Research Centre of Heart, Brain, Hormone and Healthy Aging of the University of Hong Kong
Research Grant council of Hong Kong
Funding Information:

This work was supported, in part, by the FRM (Fondation pour la Recherche Medicale, France) (Grant SPE20050303429 to FSM) and the Research Centre of Heart, Brain, Hormone and Healthy Aging of the University of Hong Kong and the Research Grant council of Hong Kong (PMV).

References

 

DC FieldValueLanguage
dc.contributor.authorMichel, FSen_HK
dc.contributor.authorMan, GSen_HK
dc.contributor.authorMan, RYKen_HK
dc.contributor.authorVanhoutte, PMen_HK
dc.date.accessioned2010-05-31T03:52:38Z-
dc.date.available2010-05-31T03:52:38Z-
dc.date.issued2008en_HK
dc.identifier.citationBritish Journal Of Pharmacology, 2008, v. 155 n. 2, p. 217-226en_HK
dc.identifier.issn0007-1188en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59556-
dc.description.abstractBackground and purpose: Experiments were designed to determine the modulation by nitric oxide (NO) and endothelium-dependent hyperpolarizations (EDHF-mediated responses) of endothelium-dependent contractions in renal arteries of normotensive and hypertensive rats. Experimental approach: Rings, with or without endothelium, of renal arteries of 8-month-old Wistar Kyoto rats (WKY) and spontaneously hypertensive rats (SHR) were suspended in myographs for isometric force recording. Key results: ACh evoked relaxations in preparations contracted with phenylephrine. L-NAME (inhibitor of NOS) attenuated (WKY) or abolished (SHR) these relaxations. TRAM-34 plus UCL 1684 (inhibitors of EDHF-mediated responses) did not decrease the relaxation, except in rings of WKY when L-NAME was also present. High concentrations of ACh caused a secondary increase in tension, augmented in rings of WKY by L-NAME or TRAM-34 plus UCL 1684. The increase in tension was prevented by indomethacin. Under baseline tension, ACh induced endothelium-dependent contractions, prevented by indomethacin (COX inhibitor) or terutroban (TP receptor antagonist). The calculated endothelium-dependent contractions were larger in rings of SHR compared with those of WKY. In preparations of SHR, the contractions were augmented by L-NAME in the presence of SC19220 (EP-1 receptor antagonist). In arteries of WKY, the endothelium-dependent contractions were augmented by TRAM-34 plus UCL 1684. The responses were reduced by SC19220. Conclusions and implications: In the renal artery of the rat, EDCF-mediated contractions are augmented by hypertension. The endothelium-dependent contractions are facilitated by NOS inhibition (in the presence of an EP-1 receptor antagonist) and by the withdrawal of EDHF-mediated responses. © 2008 Macmillan Publishers Limited All rights reserved.en_HK
dc.languageengen_HK
dc.publisherJohn Wiley & Sons Ltd. The Journal's web site is located at http://www.wiley.com/bw/journal.asp?ref=0007-1188&site=1en_HK
dc.relation.ispartofBritish Journal of Pharmacologyen_HK
dc.rightsBritish Journal of Pharmacology. Copyright © John Wiley & Sons Ltd.-
dc.subjectAChen_HK
dc.subjectCOXen_HK
dc.subjectEP-1 receptorsen_HK
dc.subjectNOSen_HK
dc.subjectTP receptorsen_HK
dc.subject.meshBiological Factors - deficiency-
dc.subject.meshEndothelium, Vascular - physiology - physiopathology-
dc.subject.meshHypertension - metabolism - physiopathology-
dc.subject.meshRenal Artery - physiopathology-
dc.subject.meshVasoconstriction - physiology-
dc.titleHypertension and the absence of EDHF-mediated responses favour endothelium-dependent contractions in renal arteries of the raten_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0007-1188&volume=155&issue=2&spage=217&epage=226&date=2008&atitle=Hypertension+and+the+absence+of+EDHF-mediated+responses+favor+endothelium-dependent+contractions+in+renal+arteries+of+the+raten_HK
dc.identifier.emailMan, RYK: rykman@hkucc.hku.hken_HK
dc.identifier.emailVanhoutte, PM: vanhoutt@hku.hken_HK
dc.identifier.authorityMan, RYK=rp00236en_HK
dc.identifier.authorityVanhoutte, PM=rp00238en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1038/bjp.2008.256en_HK
dc.identifier.pmid18574459-
dc.identifier.pmcidPMC2538696-
dc.identifier.scopuseid_2-s2.0-51349118085en_HK
dc.identifier.hkuros167577en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-51349118085&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume155en_HK
dc.identifier.issue2en_HK
dc.identifier.spage217en_HK
dc.identifier.epage226en_HK
dc.identifier.isiWOS:000258985100007-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridMichel, FS=7402556401en_HK
dc.identifier.scopusauthoridMan, GS=7003741547en_HK
dc.identifier.scopusauthoridMan, RYK=7004986435en_HK
dc.identifier.scopusauthoridVanhoutte, PM=7202304247en_HK

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