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Article: Role of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery
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TitleRole of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery
 
AuthorsSeto, SW4
Lam, HY4
Lau, WS4
Au, ALS4
Lam, TY4
Chim, SSC4
Ngai, SM4
Chan, SW3
Leung, TY4
Yeung, JHK4
Kong, SK4
Leung, GPH1
Lee, SMY2
Kwan, YW4
 
Keywords5-hydroxytryptamine
Monoamine oxidase
Preeclampsia
Umbilical artery
 
Issue Date2009
 
PublisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
 
CitationEuropean Journal Of Pharmacology, 2009, v. 605 n. 1-3, p. 129-137 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ejphar.2008.12.054
 
AbstractWe investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (Nω-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development. © 2009 Elsevier B.V. All rights reserved.
 
ISSN0014-2999
2013 Impact Factor: 2.684
2013 SCImago Journal Rankings: 1.067
 
DOIhttp://dx.doi.org/10.1016/j.ejphar.2008.12.054
 
ISI Accession Number IDWOS:000264407300021
Funding AgencyGrant Number
Li Ka Shing Institute of Health Sciences and Institute of Vascular Medicine
Faculty of Medicine
Chinese University of Hong Kong2401149
2041231
2401296
2007.1.079
RGC4107/01M
4166/02M
2140565
Funding Information:

We thank the excellent assistance provided by nurses of the operating theatres of The Prince of Wales Hospital (Hong Kong), and women who agreed to donate the umbilical cords for this study. We are grateful to Li Ka Shing Institute of Health Sciences and Institute of Vascular Medicine (Faculty of Medicine, The Chinese University of Hong Kong) for financial supports (to YW Kwan). This project is financially supported by the RGC Earmarked Grants of Hong Kong (Ref. #: 4107/01M; 4166/02M, project code: 2140565), and Direct Grants for Research (The Chinese University of Hong Kong) (Reference no.: 2401149; Project code/ID: 2041231; 2401296; Reference no.: 2007.1.079). Mr. SW Seto, Ms. Alice LS Au and Ms TY Lam are recipients of post-graduate studentships of the Department of Pharmacology (The Chinese University of Hong Kong). Provision of the Student Campus Work Scheme by the Chou's Foundation Fund, and the Student Training & Campus Service Scheme (Shaw College, The Chinese University of Hong Kong) is also appreciated. Proofreading of the manuscript by Mr. Alan HY Lam is also acknowledged.

 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorSeto, SW
 
dc.contributor.authorLam, HY
 
dc.contributor.authorLau, WS
 
dc.contributor.authorAu, ALS
 
dc.contributor.authorLam, TY
 
dc.contributor.authorChim, SSC
 
dc.contributor.authorNgai, SM
 
dc.contributor.authorChan, SW
 
dc.contributor.authorLeung, TY
 
dc.contributor.authorYeung, JHK
 
dc.contributor.authorKong, SK
 
dc.contributor.authorLeung, GPH
 
dc.contributor.authorLee, SMY
 
dc.contributor.authorKwan, YW
 
dc.date.accessioned2010-05-31T03:52:19Z
 
dc.date.available2010-05-31T03:52:19Z
 
dc.date.issued2009
 
dc.description.abstractWe investigated the role(s) of monoamine oxidases (MAOs) on the altered 5-hydroxytryptamine (5-HT, serotonin)-induced tension development of the isolated umbilical artery of preeclamptic pregnancy of Chinese women. An enhanced 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy was observed when compared with that of normal pregnancy. The enhanced component of 5-HT-induced tension development was eradicated by clorgyline (a MAO-A inhibitor). Blockade of eNOS (endothelial isoform nitric oxide synthase) (Nω-nitro-l-arginine methyl ester), 5-HT transporter (citalopram), 5-HT receptor subtypes (5HT2B, SB 204741; 5-HT2C, RS 102221; 5-HT7, SB 269970), and endothelium denudation of the umbilical artery of normal pregnancy mimicked the enhanced 5-HT-induced tension development as observed in the preeclamptic tissues. In contrast, no apparent changes in 5-HT-induced tension development of the umbilical artery of preeclamptic pregnancy were observed with the same pharmacological manipulations. A decreased protein expression levels of MAO-A and eNOS (no iNOS and MAO-B expression was detected) and no change in caveolin-1 and 5-HT transporter expression were demonstrated in the umbilical artery (endothelium intact) lysate of preeclamptic pregnancy, compared to that of the umbilical artery of normal pregnancy. Thus, in the umbilical artery of preeclamptic pregnancy, a decrease of MAO-A and eNOS protein expression levels are probably associated with, or responsible for, the exaggerated 5-HT-induced tension development. © 2009 Elsevier B.V. All rights reserved.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationEuropean Journal Of Pharmacology, 2009, v. 605 n. 1-3, p. 129-137 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.ejphar.2008.12.054
 
dc.identifier.doihttp://dx.doi.org/10.1016/j.ejphar.2008.12.054
 
dc.identifier.epage137
 
dc.identifier.hkuros157518
 
dc.identifier.isiWOS:000264407300021
Funding AgencyGrant Number
Li Ka Shing Institute of Health Sciences and Institute of Vascular Medicine
Faculty of Medicine
Chinese University of Hong Kong2401149
2041231
2401296
2007.1.079
RGC4107/01M
4166/02M
2140565
Funding Information:

We thank the excellent assistance provided by nurses of the operating theatres of The Prince of Wales Hospital (Hong Kong), and women who agreed to donate the umbilical cords for this study. We are grateful to Li Ka Shing Institute of Health Sciences and Institute of Vascular Medicine (Faculty of Medicine, The Chinese University of Hong Kong) for financial supports (to YW Kwan). This project is financially supported by the RGC Earmarked Grants of Hong Kong (Ref. #: 4107/01M; 4166/02M, project code: 2140565), and Direct Grants for Research (The Chinese University of Hong Kong) (Reference no.: 2401149; Project code/ID: 2041231; 2401296; Reference no.: 2007.1.079). Mr. SW Seto, Ms. Alice LS Au and Ms TY Lam are recipients of post-graduate studentships of the Department of Pharmacology (The Chinese University of Hong Kong). Provision of the Student Campus Work Scheme by the Chou's Foundation Fund, and the Student Training & Campus Service Scheme (Shaw College, The Chinese University of Hong Kong) is also appreciated. Proofreading of the manuscript by Mr. Alan HY Lam is also acknowledged.

 
dc.identifier.issn0014-2999
2013 Impact Factor: 2.684
2013 SCImago Journal Rankings: 1.067
 
dc.identifier.issue1-3
 
dc.identifier.openurl
 
dc.identifier.pmid19248248
 
dc.identifier.scopuseid_2-s2.0-60349130008
 
dc.identifier.spage129
 
dc.identifier.urihttp://hdl.handle.net/10722/59539
 
dc.identifier.volume605
 
dc.languageeng
 
dc.publisherElsevier BV. The Journal's web site is located at http://www.elsevier.com/locate/ejphar
 
dc.publisher.placeNetherlands
 
dc.relation.ispartofEuropean Journal of Pharmacology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsEuropean Journal of Pharmacology . Copyright © Elsevier BV.
 
dc.subject5-hydroxytryptamine
 
dc.subjectMonoamine oxidase
 
dc.subjectPreeclampsia
 
dc.subjectUmbilical artery
 
dc.titleRole of monoamine oxidases in the exaggerated 5-hydroxytryptamine-induced tension development of human isolated preeclamptic umbilical artery
 
dc.typeArticle
 
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Author Affiliations
  1. The University of Hong Kong
  2. University of Macau
  3. Hong Kong Polytechnic University
  4. Chinese University of Hong Kong