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Article: Ganoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function.

TitleGanoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function.
Authors
Issue Date2008
PublisherBioMed Central Ltd. The Journal's web site is located at http://www.jhoonline.org
Citation
Journal Of Hematology & Oncology, 2008, v. 1, p. 9 How to Cite?
AbstractBACKGROUND: Previous studies demonstrated Ganoderma lucidum polysaccharides (GL-PS), a form of bioactive beta-glucan can stimulate the maturation of monocyte-derived dendritic cells (DC). The question of how leukemic cells especially in monocytic lineage respond to GL-PS stimuli remains unclear. RESULTS: In this study, we used in vitro culture model with leukemic monocytic cell-lines THP-1 and U937 as monocytic effectors cells for proliferation responses and DCs induction. We treated the THP-1 and U937 cells with purified GL-PS (100 microg/mL) or GL-PS with GM-CSF/IL-4. GL-PS alone induced proliferative response on both THP-1 and U937 cells but only THP-1 transformed into typical DC morphology when stimulated with GL-PS plus GM-CSF/IL-4. The transformed THP-1 DCs had significant increase expression of HLA-DR, CD40, CD80 and CD86 though not as high as the extent of normal monocyte-derived DCs. They had similar antigen-uptake ability as the normal monocyte-derived DCs positive control. However, their potency in inducing allogeneic T cell proliferation was also less than that of normal monocyte-derived DCs. CONCLUSION: Our findings suggested that GL-PS could induce selected monocytic leukemic cell differentiation into DCs with immuno-stimulatory function. The possible clinical impact of using this commonly used medicinal mushroom in patients with monocytic leukemia (AML-M4 and M5) deserved further investigation.
Persistent Identifierhttp://hdl.handle.net/10722/59517
ISSN
2015 Impact Factor: 6.263
2015 SCImago Journal Rankings: 2.245
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorChan, WKen_HK
dc.contributor.authorCheung, CCen_HK
dc.contributor.authorLaw, HKen_HK
dc.contributor.authorLau, YLen_HK
dc.contributor.authorChan, GCen_HK
dc.date.accessioned2010-05-31T03:51:50Z-
dc.date.available2010-05-31T03:51:50Z-
dc.date.issued2008en_HK
dc.identifier.citationJournal Of Hematology & Oncology, 2008, v. 1, p. 9en_HK
dc.identifier.issn1756-8722en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59517-
dc.description.abstractBACKGROUND: Previous studies demonstrated Ganoderma lucidum polysaccharides (GL-PS), a form of bioactive beta-glucan can stimulate the maturation of monocyte-derived dendritic cells (DC). The question of how leukemic cells especially in monocytic lineage respond to GL-PS stimuli remains unclear. RESULTS: In this study, we used in vitro culture model with leukemic monocytic cell-lines THP-1 and U937 as monocytic effectors cells for proliferation responses and DCs induction. We treated the THP-1 and U937 cells with purified GL-PS (100 microg/mL) or GL-PS with GM-CSF/IL-4. GL-PS alone induced proliferative response on both THP-1 and U937 cells but only THP-1 transformed into typical DC morphology when stimulated with GL-PS plus GM-CSF/IL-4. The transformed THP-1 DCs had significant increase expression of HLA-DR, CD40, CD80 and CD86 though not as high as the extent of normal monocyte-derived DCs. They had similar antigen-uptake ability as the normal monocyte-derived DCs positive control. However, their potency in inducing allogeneic T cell proliferation was also less than that of normal monocyte-derived DCs. CONCLUSION: Our findings suggested that GL-PS could induce selected monocytic leukemic cell differentiation into DCs with immuno-stimulatory function. The possible clinical impact of using this commonly used medicinal mushroom in patients with monocytic leukemia (AML-M4 and M5) deserved further investigation.en_HK
dc.languageengen_HK
dc.publisherBioMed Central Ltd. The Journal's web site is located at http://www.jhoonline.orgen_HK
dc.relation.ispartofJournal of hematology & oncologyen_HK
dc.rightsJournal of Hematology & Oncology. Copyright © BioMed Central Ltd.en_HK
dc.titleGanoderma lucidum polysaccharides can induce human monocytic leukemia cells into dendritic cells with immuno-stimulatory function.en_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1756-8722&volume=1&issue=9&spage=1&epage=12&date=2008&atitle=Ganoderma+lucidum+polysaccharides+can+induce+human+monocytic+leukemia+cells+into+dendritic+cells+with+immuno-stimulatory+functionen_HK
dc.identifier.emailLau, YL:lauylung@hkucc.hku.hken_HK
dc.identifier.emailChan, GC:gcfchan@hkucc.hku.hken_HK
dc.identifier.authorityLau, YL=rp00361en_HK
dc.identifier.authorityChan, GC=rp00431en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.pmid18644156-
dc.identifier.scopuseid_2-s2.0-77953049253en_HK
dc.identifier.hkuros153948en_HK
dc.identifier.volume1en_HK
dc.identifier.spage9en_HK
dc.identifier.epage9en_HK
dc.identifier.isiWOS:000272008500001-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChan, WK=35080149500en_HK
dc.identifier.scopusauthoridCheung, CC=36902188600en_HK
dc.identifier.scopusauthoridLaw, HK=7101939394en_HK
dc.identifier.scopusauthoridLau, YL=7201403380en_HK
dc.identifier.scopusauthoridChan, GC=16160154400en_HK

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