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Article: Extracellular matrix stability of primary mammalian chondrocytes and intervertebral disc cells cultured in alginate-based microbead hydrogels

TitleExtracellular matrix stability of primary mammalian chondrocytes and intervertebral disc cells cultured in alginate-based microbead hydrogels
Authors
KeywordsAlginate microbeads
Cell encapsulation
Chondrocytes
Chondrogenic extracellular matrix
Intervertebral disc cells
Issue Date2008
PublisherCognizant Communication Corp
Citation
Cell Transplantation, 2008, v. 17 n. 10-11, p. 1181-1192 How to Cite?
AbstractThree-dimensional alginate constructs are widely used as carrier systems for transplantable cells. In the present study, we evaluated the chondrogenic matrix stability of primary rat chondrocytes and intervertebral disc (IVD) cells cultured in three different alginate-based microbead matrices to determine the influence of microenvironment on the cellular and metabolic behaviors of chondrogenic cells confined in alginate microbeads. Cells entrapped in calcium, strontium, or barium ion gelled microbeads were monitored with the live/dead dual fluorescent cell viability assay kit and the 1,9-dimethylmethylene blue (DMB) assay designed to evaluate sulfated glycosaminoglycan (s-GAG) production. Expression of chondrogenic extracellular matrix (ECM) synthesis was further evaluated by semiquantitative RT-PCR of sox9, type II collagen, and aggrecan mRNAs. Results indicate that Ca and Sr alginate maintained significantly higher population of living cells compared to Ba alginate (p < 0.05). Production of s-GAG was similarly higher in Ca and Sr alginate microbead cultures compared to Ba alginate microbeads. Although there was no significant difference between strontium and calcium up to day 14 of culture, Sr alginate showed remarkably improved cellular and metabolic activities on long-term cultures, with chondrocytes expressing as much as 31% and 44% greater s-GAG compared to calcium and barium constructs, respectively, while IVD cells expressed 63% and 74% greater s-GAG compared to calcium and barium constructs, respectively, on day 28. These findings indicate that Sr alginate represent a significant improvement over Ca- and Ba alginate microbeads for the maintenance of chondrogenic phenotype of primary chondrocytes and IVD cells. Copyright © 2008 Cognizant Comm. Corp.
Persistent Identifierhttp://hdl.handle.net/10722/59476
ISSN
2015 Impact Factor: 3.427
2015 SCImago Journal Rankings: 1.161
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorAbbah, SAen_HK
dc.contributor.authorLu, WWen_HK
dc.contributor.authorPeng, SLen_HK
dc.contributor.authorAladin, DMKen_HK
dc.contributor.authorLi, ZYen_HK
dc.contributor.authorTam, WKen_HK
dc.contributor.authorCheung, KMCen_HK
dc.contributor.authorLuk, KDKen_HK
dc.contributor.authorZhou, GQen_HK
dc.date.accessioned2010-05-31T03:50:59Z-
dc.date.available2010-05-31T03:50:59Z-
dc.date.issued2008en_HK
dc.identifier.citationCell Transplantation, 2008, v. 17 n. 10-11, p. 1181-1192en_HK
dc.identifier.issn0963-6897en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59476-
dc.description.abstractThree-dimensional alginate constructs are widely used as carrier systems for transplantable cells. In the present study, we evaluated the chondrogenic matrix stability of primary rat chondrocytes and intervertebral disc (IVD) cells cultured in three different alginate-based microbead matrices to determine the influence of microenvironment on the cellular and metabolic behaviors of chondrogenic cells confined in alginate microbeads. Cells entrapped in calcium, strontium, or barium ion gelled microbeads were monitored with the live/dead dual fluorescent cell viability assay kit and the 1,9-dimethylmethylene blue (DMB) assay designed to evaluate sulfated glycosaminoglycan (s-GAG) production. Expression of chondrogenic extracellular matrix (ECM) synthesis was further evaluated by semiquantitative RT-PCR of sox9, type II collagen, and aggrecan mRNAs. Results indicate that Ca and Sr alginate maintained significantly higher population of living cells compared to Ba alginate (p < 0.05). Production of s-GAG was similarly higher in Ca and Sr alginate microbead cultures compared to Ba alginate microbeads. Although there was no significant difference between strontium and calcium up to day 14 of culture, Sr alginate showed remarkably improved cellular and metabolic activities on long-term cultures, with chondrocytes expressing as much as 31% and 44% greater s-GAG compared to calcium and barium constructs, respectively, while IVD cells expressed 63% and 74% greater s-GAG compared to calcium and barium constructs, respectively, on day 28. These findings indicate that Sr alginate represent a significant improvement over Ca- and Ba alginate microbeads for the maintenance of chondrogenic phenotype of primary chondrocytes and IVD cells. Copyright © 2008 Cognizant Comm. Corp.en_HK
dc.languageengen_HK
dc.publisherCognizant Communication Corpen_HK
dc.relation.ispartofCell Transplantationen_HK
dc.subjectAlginate microbeadsen_HK
dc.subjectCell encapsulationen_HK
dc.subjectChondrocytesen_HK
dc.subjectChondrogenic extracellular matrixen_HK
dc.subjectIntervertebral disc cellsen_HK
dc.titleExtracellular matrix stability of primary mammalian chondrocytes and intervertebral disc cells cultured in alginate-based microbead hydrogelsen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0963-6897&volume=17 &issue=10-11&spage=1181&epage=92&date=2008&atitle=Extracellular+matrix+stability+of+primary+mammalian+chondrocytes+and+intervertebral+disc+cells+cultured+in+alginate-based+microbead+hydrogels.en_HK
dc.identifier.emailLu, WW:wwlu@hku.hken_HK
dc.identifier.emailCheung, KMC:cheungmc@hku.hken_HK
dc.identifier.emailLuk, KDK:hcm21000@hku.hken_HK
dc.identifier.emailZhou, GQ:wormoscz@gmail.comen_HK
dc.identifier.authorityLu, WW=rp00411en_HK
dc.identifier.authorityCheung, KMC=rp00387en_HK
dc.identifier.authorityLuk, KDK=rp00333en_HK
dc.identifier.authorityZhou, GQ=rp00527en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.3727/096368908787236648-
dc.identifier.pmid19181212-
dc.identifier.scopuseid_2-s2.0-60549096691en_HK
dc.identifier.hkuros166608en_HK
dc.identifier.hkuros144001-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-60549096691&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume17en_HK
dc.identifier.issue10-11en_HK
dc.identifier.spage1181en_HK
dc.identifier.epage1192en_HK
dc.identifier.isiWOS:000262771400008-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridAbbah, SA=14032930600en_HK
dc.identifier.scopusauthoridLu, WW=7404215221en_HK
dc.identifier.scopusauthoridPeng, SL=13402746900en_HK
dc.identifier.scopusauthoridAladin, DMK=23491673700en_HK
dc.identifier.scopusauthoridLi, ZY=35784563200en_HK
dc.identifier.scopusauthoridTam, WK=36961689000en_HK
dc.identifier.scopusauthoridCheung, KMC=7402406754en_HK
dc.identifier.scopusauthoridLuk, KDK=7201921573en_HK
dc.identifier.scopusauthoridZhou, GQ=23394245100en_HK

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