File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: BK Virus Nephropathy Due to KOM-3 Strain

TitleBK Virus Nephropathy Due to KOM-3 Strain
Authors
KeywordsBK virus nephropathy
renal transplant
Issue Date2009
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkd
Citation
American Journal Of Kidney Diseases, 2009, v. 54 n. 1, p. 122-126 How to Cite?
AbstractInterstitial nephritis caused by BK polyomavirus is an important complication of kidney transplantation. A diagnosis of BK virus nephropathy is established by a combination of characteristic histological, immunostaining, and ultrastructural findings. We report the first documented case of BK virus nephropathy caused by the KOM-3 strain in a patient after kidney transplantation. The biopsy specimen showed the characteristic histological and ultrastructural findings of BK virus, but was negative on immunostaining with a monoclonal antibody directed against BK virus large T antigen (LTag). Kidney tissue was subjected to polymerase chain reaction amplification using BK virus LTag-specific primers followed by DNA sequencing. Sequence results showed 100% homology to the KOM-3 strain, which has a 4-amino acid deletion in the C terminus of LTag compared with the reference sequence DUN strain. This deletion can explain the negative immunostaining results because the monoclonal antibody is directed against an epitope in this region. The patient lost his graft 2 months after diagnosis. Pathologists should be aware of this potential pitfall in interpreting immunostaining for BK virus. The incidence and prognostic implications of KOM-3 strain require additional studies. © 2009 National Kidney Foundation, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/59447
ISSN
2014 Impact Factor: 5.900
2014 SCImago Journal Rankings: 1.651
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, GSWen_HK
dc.contributor.authorTsoi, HWen_HK
dc.contributor.authorWong, SSYen_HK
dc.contributor.authorLi, CLen_HK
dc.contributor.authorTse, Hen_HK
dc.contributor.authorUn I, Ken_HK
dc.contributor.authorYuen, KYen_HK
dc.contributor.authorChan, KWen_HK
dc.date.accessioned2010-05-31T03:50:16Z-
dc.date.available2010-05-31T03:50:16Z-
dc.date.issued2009en_HK
dc.identifier.citationAmerican Journal Of Kidney Diseases, 2009, v. 54 n. 1, p. 122-126en_HK
dc.identifier.issn0272-6386en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59447-
dc.description.abstractInterstitial nephritis caused by BK polyomavirus is an important complication of kidney transplantation. A diagnosis of BK virus nephropathy is established by a combination of characteristic histological, immunostaining, and ultrastructural findings. We report the first documented case of BK virus nephropathy caused by the KOM-3 strain in a patient after kidney transplantation. The biopsy specimen showed the characteristic histological and ultrastructural findings of BK virus, but was negative on immunostaining with a monoclonal antibody directed against BK virus large T antigen (LTag). Kidney tissue was subjected to polymerase chain reaction amplification using BK virus LTag-specific primers followed by DNA sequencing. Sequence results showed 100% homology to the KOM-3 strain, which has a 4-amino acid deletion in the C terminus of LTag compared with the reference sequence DUN strain. This deletion can explain the negative immunostaining results because the monoclonal antibody is directed against an epitope in this region. The patient lost his graft 2 months after diagnosis. Pathologists should be aware of this potential pitfall in interpreting immunostaining for BK virus. The incidence and prognostic implications of KOM-3 strain require additional studies. © 2009 National Kidney Foundation, Inc.en_HK
dc.languageengen_HK
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkden_HK
dc.relation.ispartofAmerican Journal of Kidney Diseasesen_HK
dc.subjectBK virus nephropathyen_HK
dc.subjectrenal transplanten_HK
dc.subject.meshAdulten_HK
dc.subject.meshAntibodies, Monoclonal - immunologyen_HK
dc.subject.meshAntigens, Viral, Tumor - genetics - immunologyen_HK
dc.subject.meshBK Virus - genetics - immunology - pathogenicityen_HK
dc.subject.meshBase Sequenceen_HK
dc.subject.meshGraft Rejectionen_HK
dc.subject.meshHumansen_HK
dc.subject.meshKidney Failure, Chronic - surgeryen_HK
dc.subject.meshKidney Transplantation - adverse effectsen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNephritis, Interstitial - diagnosis - virologyen_HK
dc.subject.meshPolyomavirus Infections - diagnosis - etiologyen_HK
dc.subject.meshPrognosisen_HK
dc.subject.meshTumor Virus Infections - diagnosis - etiologyen_HK
dc.titleBK Virus Nephropathy Due to KOM-3 Strainen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0272-6386&volume=54&issue=1&spage=122&epage=126&date=2008&atitle=BK+virus+nephropathy+due+to+KOM-3+strain.en_HK
dc.identifier.emailTsoi, HW:hwtsoi@hkucc.hku.hken_HK
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_HK
dc.identifier.emailTse, H:herman@graduate.hku.hken_HK
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_HK
dc.identifier.emailChan, KW:hrmtckw@hku.hken_HK
dc.identifier.authorityTsoi, HW=rp00439en_HK
dc.identifier.authorityWong, SSY=rp00395en_HK
dc.identifier.authorityTse, H=rp00519en_HK
dc.identifier.authorityYuen, KY=rp00366en_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1053/j.ajkd.2008.09.014en_HK
dc.identifier.pmid19022546en_HK
dc.identifier.scopuseid_2-s2.0-67449116335en_HK
dc.identifier.hkuros167072en_HK
dc.identifier.hkuros159779-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67449116335&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume54en_HK
dc.identifier.issue1en_HK
dc.identifier.spage122en_HK
dc.identifier.epage126en_HK
dc.identifier.isiWOS:000270214400019-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, GSW=7202355321en_HK
dc.identifier.scopusauthoridTsoi, HW=6603822102en_HK
dc.identifier.scopusauthoridWong, SSY=13310021400en_HK
dc.identifier.scopusauthoridLi, CL=26653600300en_HK
dc.identifier.scopusauthoridTse, H=7006070596en_HK
dc.identifier.scopusauthoridUn I, K=26653738200en_HK
dc.identifier.scopusauthoridYuen, KY=36078079100en_HK
dc.identifier.scopusauthoridChan, KW=16444133100en_HK

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats