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Article: BK Virus Nephropathy Due to KOM-3 Strain
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TitleBK Virus Nephropathy Due to KOM-3 Strain
 
AuthorsChan, GSW2
Tsoi, HW2
Wong, SSY2
Li, CL1
Tse, H2
Un I, K1
Yuen, KY2
Chan, KW2
 
KeywordsBK virus nephropathy
renal transplant
 
Issue Date2009
 
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkd
 
CitationAmerican Journal Of Kidney Diseases, 2009, v. 54 n. 1, p. 122-126 [How to Cite?]
DOI: http://dx.doi.org/10.1053/j.ajkd.2008.09.014
 
AbstractInterstitial nephritis caused by BK polyomavirus is an important complication of kidney transplantation. A diagnosis of BK virus nephropathy is established by a combination of characteristic histological, immunostaining, and ultrastructural findings. We report the first documented case of BK virus nephropathy caused by the KOM-3 strain in a patient after kidney transplantation. The biopsy specimen showed the characteristic histological and ultrastructural findings of BK virus, but was negative on immunostaining with a monoclonal antibody directed against BK virus large T antigen (LTag). Kidney tissue was subjected to polymerase chain reaction amplification using BK virus LTag-specific primers followed by DNA sequencing. Sequence results showed 100% homology to the KOM-3 strain, which has a 4-amino acid deletion in the C terminus of LTag compared with the reference sequence DUN strain. This deletion can explain the negative immunostaining results because the monoclonal antibody is directed against an epitope in this region. The patient lost his graft 2 months after diagnosis. Pathologists should be aware of this potential pitfall in interpreting immunostaining for BK virus. The incidence and prognostic implications of KOM-3 strain require additional studies. © 2009 National Kidney Foundation, Inc.
 
ISSN0272-6386
2013 Impact Factor: 5.756
 
DOIhttp://dx.doi.org/10.1053/j.ajkd.2008.09.014
 
ISI Accession Number IDWOS:000270214400019
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorChan, GSW
 
dc.contributor.authorTsoi, HW
 
dc.contributor.authorWong, SSY
 
dc.contributor.authorLi, CL
 
dc.contributor.authorTse, H
 
dc.contributor.authorUn I, K
 
dc.contributor.authorYuen, KY
 
dc.contributor.authorChan, KW
 
dc.date.accessioned2010-05-31T03:50:16Z
 
dc.date.available2010-05-31T03:50:16Z
 
dc.date.issued2009
 
dc.description.abstractInterstitial nephritis caused by BK polyomavirus is an important complication of kidney transplantation. A diagnosis of BK virus nephropathy is established by a combination of characteristic histological, immunostaining, and ultrastructural findings. We report the first documented case of BK virus nephropathy caused by the KOM-3 strain in a patient after kidney transplantation. The biopsy specimen showed the characteristic histological and ultrastructural findings of BK virus, but was negative on immunostaining with a monoclonal antibody directed against BK virus large T antigen (LTag). Kidney tissue was subjected to polymerase chain reaction amplification using BK virus LTag-specific primers followed by DNA sequencing. Sequence results showed 100% homology to the KOM-3 strain, which has a 4-amino acid deletion in the C terminus of LTag compared with the reference sequence DUN strain. This deletion can explain the negative immunostaining results because the monoclonal antibody is directed against an epitope in this region. The patient lost his graft 2 months after diagnosis. Pathologists should be aware of this potential pitfall in interpreting immunostaining for BK virus. The incidence and prognostic implications of KOM-3 strain require additional studies. © 2009 National Kidney Foundation, Inc.
 
dc.description.naturelink_to_subscribed_fulltext
 
dc.identifier.citationAmerican Journal Of Kidney Diseases, 2009, v. 54 n. 1, p. 122-126 [How to Cite?]
DOI: http://dx.doi.org/10.1053/j.ajkd.2008.09.014
 
dc.identifier.doihttp://dx.doi.org/10.1053/j.ajkd.2008.09.014
 
dc.identifier.epage126
 
dc.identifier.hkuros167072
 
dc.identifier.hkuros159779
 
dc.identifier.isiWOS:000270214400019
 
dc.identifier.issn0272-6386
2013 Impact Factor: 5.756
 
dc.identifier.issue1
 
dc.identifier.openurl
 
dc.identifier.pmid19022546
 
dc.identifier.scopuseid_2-s2.0-67449116335
 
dc.identifier.spage122
 
dc.identifier.urihttp://hdl.handle.net/10722/59447
 
dc.identifier.volume54
 
dc.languageeng
 
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkd
 
dc.publisher.placeUnited States
 
dc.relation.ispartofAmerican Journal of Kidney Diseases
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAdult
 
dc.subject.meshAntibodies, Monoclonal - immunology
 
dc.subject.meshAntigens, Viral, Tumor - genetics - immunology
 
dc.subject.meshBK Virus - genetics - immunology - pathogenicity
 
dc.subject.meshBase Sequence
 
dc.subject.meshGraft Rejection
 
dc.subject.meshHumans
 
dc.subject.meshKidney Failure, Chronic - surgery
 
dc.subject.meshKidney Transplantation - adverse effects
 
dc.subject.meshMale
 
dc.subject.meshMolecular Sequence Data
 
dc.subject.meshNephritis, Interstitial - diagnosis - virology
 
dc.subject.meshPolyomavirus Infections - diagnosis - etiology
 
dc.subject.meshPrognosis
 
dc.subject.meshTumor Virus Infections - diagnosis - etiology
 
dc.subjectBK virus nephropathy
 
dc.subjectrenal transplant
 
dc.titleBK Virus Nephropathy Due to KOM-3 Strain
 
dc.typeArticle
 
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<contributor.author>Tse, H</contributor.author>
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<description.abstract>Interstitial nephritis caused by BK polyomavirus is an important complication of kidney transplantation. A diagnosis of BK virus nephropathy is established by a combination of characteristic histological, immunostaining, and ultrastructural findings. We report the first documented case of BK virus nephropathy caused by the KOM-3 strain in a patient after kidney transplantation. The biopsy specimen showed the characteristic histological and ultrastructural findings of BK virus, but was negative on immunostaining with a monoclonal antibody directed against BK virus large T antigen (LTag). Kidney tissue was subjected to polymerase chain reaction amplification using BK virus LTag-specific primers followed by DNA sequencing. Sequence results showed 100% homology to the KOM-3 strain, which has a 4-amino acid deletion in the C terminus of LTag compared with the reference sequence DUN strain. This deletion can explain the negative immunostaining results because the monoclonal antibody is directed against an epitope in this region. The patient lost his graft 2 months after diagnosis. Pathologists should be aware of this potential pitfall in interpreting immunostaining for BK virus. The incidence and prognostic implications of KOM-3 strain require additional studies. &#169; 2009 National Kidney Foundation, Inc.</description.abstract>
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Author Affiliations
  1. Centro Hospitalar Conde De S Januario
  2. Queen Mary Hospital