Article: BK Virus Nephropathy Due to KOM-3 Strain

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TitleBK Virus Nephropathy Due to KOM-3 Strain
AuthorsChan, GSW2
Tsoi, HW2
Wong, SSY2
Li, CL1
Tse, H2
Un I, K1
Yuen, KY2
Chan, KW2
KeywordsBK virus nephropathy
renal transplant
Issue Date2009
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkd
CitationAmerican Journal Of Kidney Diseases, 2009, v. 54 n. 1, p. 122-126 [How to Cite?]
DOI: http://dx.doi.org/10.1053/j.ajkd.2008.09.014
AbstractInterstitial nephritis caused by BK polyomavirus is an important complication of kidney transplantation. A diagnosis of BK virus nephropathy is established by a combination of characteristic histological, immunostaining, and ultrastructural findings. We report the first documented case of BK virus nephropathy caused by the KOM-3 strain in a patient after kidney transplantation. The biopsy specimen showed the characteristic histological and ultrastructural findings of BK virus, but was negative on immunostaining with a monoclonal antibody directed against BK virus large T antigen (LTag). Kidney tissue was subjected to polymerase chain reaction amplification using BK virus LTag-specific primers followed by DNA sequencing. Sequence results showed 100% homology to the KOM-3 strain, which has a 4-amino acid deletion in the C terminus of LTag compared with the reference sequence DUN strain. This deletion can explain the negative immunostaining results because the monoclonal antibody is directed against an epitope in this region. The patient lost his graft 2 months after diagnosis. Pathologists should be aware of this potential pitfall in interpreting immunostaining for BK virus. The incidence and prognostic implications of KOM-3 strain require additional studies. © 2009 National Kidney Foundation, Inc.
ISSN0272-6386
2011 Impact Factor: 5.434
2011 SCImago Journal Rankings: 0.407
DOIhttp://dx.doi.org/10.1053/j.ajkd.2008.09.014
ISI Accession Number IDWOS:000270214400019
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChan, GSW
dc.contributor.authorTsoi, HW
dc.contributor.authorWong, SSY
dc.contributor.authorLi, CL
dc.contributor.authorTse, H
dc.contributor.authorUn I, K
dc.contributor.authorYuen, KY
dc.contributor.authorChan, KW
dc.date.accessioned2010-05-31T03:50:16Z
dc.date.available2010-05-31T03:50:16Z
dc.date.issued2009
dc.description.abstractInterstitial nephritis caused by BK polyomavirus is an important complication of kidney transplantation. A diagnosis of BK virus nephropathy is established by a combination of characteristic histological, immunostaining, and ultrastructural findings. We report the first documented case of BK virus nephropathy caused by the KOM-3 strain in a patient after kidney transplantation. The biopsy specimen showed the characteristic histological and ultrastructural findings of BK virus, but was negative on immunostaining with a monoclonal antibody directed against BK virus large T antigen (LTag). Kidney tissue was subjected to polymerase chain reaction amplification using BK virus LTag-specific primers followed by DNA sequencing. Sequence results showed 100% homology to the KOM-3 strain, which has a 4-amino acid deletion in the C terminus of LTag compared with the reference sequence DUN strain. This deletion can explain the negative immunostaining results because the monoclonal antibody is directed against an epitope in this region. The patient lost his graft 2 months after diagnosis. Pathologists should be aware of this potential pitfall in interpreting immunostaining for BK virus. The incidence and prognostic implications of KOM-3 strain require additional studies. © 2009 National Kidney Foundation, Inc.
dc.description.naturelink_to_subscribed_fulltext
dc.identifier.citationAmerican Journal Of Kidney Diseases, 2009, v. 54 n. 1, p. 122-126 [How to Cite?]
DOI: http://dx.doi.org/10.1053/j.ajkd.2008.09.014
dc.identifier.doihttp://dx.doi.org/10.1053/j.ajkd.2008.09.014
dc.identifier.epage126
dc.identifier.hkuros167072
dc.identifier.hkuros159779
dc.identifier.isiWOS:000270214400019
dc.identifier.issn0272-6386
2011 Impact Factor: 5.434
2011 SCImago Journal Rankings: 0.407
dc.identifier.issue1
dc.identifier.openurl
dc.identifier.pmid19022546
dc.identifier.scopuseid_2-s2.0-67449116335
dc.identifier.spage122
dc.identifier.urihttp://hdl.handle.net/10722/59447
dc.identifier.volume54
dc.languageeng
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkd
dc.publisher.placeUnited States
dc.relation.ispartofAmerican Journal of Kidney Diseases
dc.relation.referencesReferences in Scopus
dc.subject.meshAdult
dc.subject.meshAntibodies, Monoclonal - immunology
dc.subject.meshAntigens, Viral, Tumor - genetics - immunology
dc.subject.meshBK Virus - genetics - immunology - pathogenicity
dc.subject.meshBase Sequence
dc.subject.meshGraft Rejection
dc.subject.meshHumans
dc.subject.meshKidney Failure, Chronic - surgery
dc.subject.meshKidney Transplantation - adverse effects
dc.subject.meshMale
dc.subject.meshMolecular Sequence Data
dc.subject.meshNephritis, Interstitial - diagnosis - virology
dc.subject.meshPolyomavirus Infections - diagnosis - etiology
dc.subject.meshPrognosis
dc.subject.meshTumor Virus Infections - diagnosis - etiology
dc.subjectBK virus nephropathy
dc.subjectrenal transplant
dc.titleBK Virus Nephropathy Due to KOM-3 Strain
dc.typeArticle
Author Affiliations
  1. Centro Hospitalar Conde De S Januario
  2. Queen Mary Hospital