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Article: Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein

TitleSevere acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein
Authors
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
Citation
Virology, 2009, v. 388 n. 2, p. 279-285 How to Cite?
Abstract
Eight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein. © 2009 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/59439
ISSN
2013 Impact Factor: 3.278
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30325018
30530700
30623003
30421005
Technology Commission of Shanghai Municipality07DZ22916
064319034
04DZI4902
04DZI9108
06DZ22032
04DZ19112
National 863 key project2006AA02A27
Sino-Germany center on SARS projectGZ238(202/11)
Funding Information:

We thank Prof. Vincent Deubel, Prof. Paul Zhou, Prof. Jing Zhong, Prof. Ke Lan, (institute Pasteur of Shanghai, CAS, China), Prof. Shi-Shan Yuan (Shanghai Institute of Animal Parasitology, Chinese Academy of Agriculture Science), Prof. Xue-Liang Zhu (Shanghai institute of Biological Science), Dr. Sheri Skinner (USA), Prof. Hao Shen (USA) and Prof. Hans Klenk (Marburg University, Germany) for reviewing the manuscript and for constructive suggestions. We thank Mr. Yi-Min Wang (Freiburg University, Germany) for helpful comments on this study. This work was supported by grants from the National Natural Science Foundation of China (30325018, 30530700, 30623003 and 30421005) and CAS project (KSCX1-YW-R-43), grants from the Technology Commission of Shanghai Municipality (07DZ22916, 064319034, 04DZI4902, 04DZI9108, 06DZ22032 and 04DZ19112), a grant from National 863 key project (2006AA02A27), a grant from the Sino-Germany center on SARS project (GZ238(202/11)), a grant from E-institutes of the Shanghai Universities Immunology Division and a grant from Li Kha Shing Foundation.

References

 

Author Affiliations
  1. The University of Hong Kong
  2. Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences
  3. Shanghai University
  4. Academy of Military Medical Science China
  5. Chinese Academy of Sciences
  6. Shanghai Institute for Biological Sciences Chinese Academy of Sciences
DC FieldValueLanguage
dc.contributor.authorXu, Ken_HK
dc.contributor.authorZheng, BJen_HK
dc.contributor.authorZeng, Ren_HK
dc.contributor.authorLu, Wen_HK
dc.contributor.authorLin, YPen_HK
dc.contributor.authorXue, Len_HK
dc.contributor.authorLi, Len_HK
dc.contributor.authorYang, LLen_HK
dc.contributor.authorXu, Cen_HK
dc.contributor.authorDai, Jen_HK
dc.contributor.authorWang, Fen_HK
dc.contributor.authorLi, Qen_HK
dc.contributor.authorDong, QXen_HK
dc.contributor.authorYang, RFen_HK
dc.contributor.authorWu, JRen_HK
dc.contributor.authorSun, Ben_HK
dc.date.accessioned2010-05-31T03:50:07Z-
dc.date.available2010-05-31T03:50:07Z-
dc.date.issued2009en_HK
dc.identifier.citationVirology, 2009, v. 388 n. 2, p. 279-285en_HK
dc.identifier.issn0042-6822en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59439-
dc.description.abstractEight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein. © 2009 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_HK
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviroen_HK
dc.relation.ispartofVirologyen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshAntibodies, Viral - genetics - metabolismen_HK
dc.subject.meshCell Lineen_HK
dc.subject.meshCercopithecus aethiopsen_HK
dc.subject.meshDNA, Viral - geneticsen_HK
dc.subject.meshMiceen_HK
dc.subject.meshOpen Reading Frames - geneticsen_HK
dc.subject.meshPlasmidsen_HK
dc.subject.meshRNA, Messenger - chemistry - geneticsen_HK
dc.subject.meshSARS Virus - genetics - immunology - metabolismen_HK
dc.subject.meshVero Cellsen_HK
dc.subject.meshViral Structural Proteins - genetics - metabolismen_HK
dc.subject.meshVirion - genetics - metabolismen_HK
dc.titleSevere acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated proteinen_HK
dc.typeArticleen_HK
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=0042-6822&volume=388&issue=2&spage=279&epage=85&date=2009&atitle=Severe+Acute+Respiratory+Syndrome+Coronavirus+Accessory+Protein+9b+Is+a+Virion-Associated+Protein.+en_HK
dc.identifier.emailZheng, BJ:bzheng@hkucc.hku.hken_HK
dc.identifier.authorityZheng, BJ=rp00353en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.virol.2009.03.032en_HK
dc.identifier.pmid19394665en_HK
dc.identifier.scopuseid_2-s2.0-67349236733en_HK
dc.identifier.hkuros156490en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-67349236733&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume388en_HK
dc.identifier.issue2en_HK
dc.identifier.spage279en_HK
dc.identifier.epage285en_HK
dc.identifier.isiWOS:000266621400006-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridXu, K=7403282210en_HK
dc.identifier.scopusauthoridZheng, BJ=7201780588en_HK
dc.identifier.scopusauthoridZeng, R=35194877500en_HK
dc.identifier.scopusauthoridLu, W=36077781100en_HK
dc.identifier.scopusauthoridLin, YP=8591935100en_HK
dc.identifier.scopusauthoridXue, L=26649694000en_HK
dc.identifier.scopusauthoridLi, L=36064664900en_HK
dc.identifier.scopusauthoridYang, LL=31367697800en_HK
dc.identifier.scopusauthoridXu, C=37961070500en_HK
dc.identifier.scopusauthoridDai, J=35329114500en_HK
dc.identifier.scopusauthoridWang, F=23013658000en_HK
dc.identifier.scopusauthoridLi, Q=36067406200en_HK
dc.identifier.scopusauthoridDong, QX=24480732100en_HK
dc.identifier.scopusauthoridYang, RF=7403924666en_HK
dc.identifier.scopusauthoridWu, JR=7409253402en_HK
dc.identifier.scopusauthoridSun, B=24734369900en_HK
dc.identifier.citeulike4828419-

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