Article: Severe acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein

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TitleSevere acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein
AuthorsXu, K2 5
Zheng, BJ1
Zeng, R6
Lu, W2 5
Lin, YP1
Xue, L2
Li, L5
Yang, LL5
Xu, C5
Dai, J6
Wang, F5
Li, Q5
Dong, QX2
Yang, RF4
Wu, JR2
Sun, B2 3 5
Issue Date2009
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
CitationVirology, 2009, v. 388 n. 2, p. 279-285 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virol.2009.03.032
AbstractEight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein. © 2009 Elsevier Inc. All rights reserved.
ISSN0042-6822
2011 Impact Factor: 3.351
2011 SCImago Journal Rankings: 0.355
DOIhttp://dx.doi.org/10.1016/j.virol.2009.03.032
ISI Accession Number IDWOS:000266621400006
Funding AgencyGrant Number
National Natural Science Foundation of China30325018
30530700
30623003
30421005
Technology Commission of Shanghai Municipality07DZ22916
064319034
04DZI4902
04DZI9108
06DZ22032
04DZ19112
National 863 key project2006AA02A27
Sino-Germany center on SARS projectGZ238(202/11)
Funding Information:

We thank Prof. Vincent Deubel, Prof. Paul Zhou, Prof. Jing Zhong, Prof. Ke Lan, (institute Pasteur of Shanghai, CAS, China), Prof. Shi-Shan Yuan (Shanghai Institute of Animal Parasitology, Chinese Academy of Agriculture Science), Prof. Xue-Liang Zhu (Shanghai institute of Biological Science), Dr. Sheri Skinner (USA), Prof. Hao Shen (USA) and Prof. Hans Klenk (Marburg University, Germany) for reviewing the manuscript and for constructive suggestions. We thank Mr. Yi-Min Wang (Freiburg University, Germany) for helpful comments on this study. This work was supported by grants from the National Natural Science Foundation of China (30325018, 30530700, 30623003 and 30421005) and CAS project (KSCX1-YW-R-43), grants from the Technology Commission of Shanghai Municipality (07DZ22916, 064319034, 04DZI4902, 04DZI9108, 06DZ22032 and 04DZ19112), a grant from National 863 key project (2006AA02A27), a grant from the Sino-Germany center on SARS project (GZ238(202/11)), a grant from E-institutes of the Shanghai Universities Immunology Division and a grant from Li Kha Shing Foundation.

ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorXu, K
dc.contributor.authorZheng, BJ
dc.contributor.authorZeng, R
dc.contributor.authorLu, W
dc.contributor.authorLin, YP
dc.contributor.authorXue, L
dc.contributor.authorLi, L
dc.contributor.authorYang, LL
dc.contributor.authorXu, C
dc.contributor.authorDai, J
dc.contributor.authorWang, F
dc.contributor.authorLi, Q
dc.contributor.authorDong, QX
dc.contributor.authorYang, RF
dc.contributor.authorWu, JR
dc.contributor.authorSun, B
dc.date.accessioned2010-05-31T03:50:07Z
dc.date.available2010-05-31T03:50:07Z
dc.date.issued2009
dc.description.abstractEight accessory proteins have been identified in severe acute respiratory syndrome-associated coronavirus (SARS-CoV). They are believed to play roles in the viral life cycle and may contribute to the pathogenesis and virulence. ORF9b as one of these accessory proteins is located in subgenomic mRNA9 and encodes a 98 amino acid protein. However, whether 9b protein is a structural component of SARS-CoV particles remains unknown. In this study, we demonstrate that 9b protein is translated from bicistronic mRNA9 via leaky ribosome scanning and it is incorporated into both virus-like particles (VLPs) and purified SARS-CoV virions. Further analysis shows that sufficient incorporation of 9b protein into VLPs is dependent upon the co-expression of E and M proteins, but not upon the presence of either S or N protein. Our data indicate that 9b protein of SARS-CoV is another virion-associated accessory protein. This finding will lead to a better understanding of the properties of the SARS-CoV 9b protein. © 2009 Elsevier Inc. All rights reserved.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationVirology, 2009, v. 388 n. 2, p. 279-285 [How to Cite?]
DOI: http://dx.doi.org/10.1016/j.virol.2009.03.032
dc.identifier.citeulike4828419
dc.identifier.doihttp://dx.doi.org/10.1016/j.virol.2009.03.032
dc.identifier.epage285
dc.identifier.hkuros156490
dc.identifier.isiWOS:000266621400006
Funding AgencyGrant Number
National Natural Science Foundation of China30325018
30530700
30623003
30421005
Technology Commission of Shanghai Municipality07DZ22916
064319034
04DZI4902
04DZI9108
06DZ22032
04DZ19112
National 863 key project2006AA02A27
Sino-Germany center on SARS projectGZ238(202/11)
Funding Information:

We thank Prof. Vincent Deubel, Prof. Paul Zhou, Prof. Jing Zhong, Prof. Ke Lan, (institute Pasteur of Shanghai, CAS, China), Prof. Shi-Shan Yuan (Shanghai Institute of Animal Parasitology, Chinese Academy of Agriculture Science), Prof. Xue-Liang Zhu (Shanghai institute of Biological Science), Dr. Sheri Skinner (USA), Prof. Hao Shen (USA) and Prof. Hans Klenk (Marburg University, Germany) for reviewing the manuscript and for constructive suggestions. We thank Mr. Yi-Min Wang (Freiburg University, Germany) for helpful comments on this study. This work was supported by grants from the National Natural Science Foundation of China (30325018, 30530700, 30623003 and 30421005) and CAS project (KSCX1-YW-R-43), grants from the Technology Commission of Shanghai Municipality (07DZ22916, 064319034, 04DZI4902, 04DZI9108, 06DZ22032 and 04DZ19112), a grant from National 863 key project (2006AA02A27), a grant from the Sino-Germany center on SARS project (GZ238(202/11)), a grant from E-institutes of the Shanghai Universities Immunology Division and a grant from Li Kha Shing Foundation.

dc.identifier.issn0042-6822
2011 Impact Factor: 3.351
2011 SCImago Journal Rankings: 0.355
dc.identifier.issue2
dc.identifier.openurl
dc.identifier.pmid19394665
dc.identifier.scopuseid_2-s2.0-67349236733
dc.identifier.spage279
dc.identifier.urihttp://hdl.handle.net/10722/59439
dc.identifier.volume388
dc.languageeng
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yviro
dc.publisher.placeUnited States
dc.relation.ispartofVirology
dc.relation.referencesReferences in Scopus
dc.subject.meshAnimals
dc.subject.meshAntibodies, Viral - genetics - metabolism
dc.subject.meshCell Line
dc.subject.meshCercopithecus aethiops
dc.subject.meshDNA, Viral - genetics
dc.subject.meshMice
dc.subject.meshOpen Reading Frames - genetics
dc.subject.meshPlasmids
dc.subject.meshRNA, Messenger - chemistry - genetics
dc.subject.meshSARS Virus - genetics - immunology - metabolism
dc.subject.meshVero Cells
dc.subject.meshViral Structural Proteins - genetics - metabolism
dc.subject.meshVirion - genetics - metabolism
dc.titleSevere acute respiratory syndrome coronavirus accessory protein 9b is a virion-associated protein
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong
  2. Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences Chinese Academy of Sciences
  3. Shanghai University
  4. Academy of Military Medical Science China
  5. Chinese Academy of Sciences
  6. Shanghai Institute for Biological Sciences Chinese Academy of Sciences