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Article: Identification of Amino Acid Substitutions in Avian Influenza Virus (H5N1) Matrix Protein 1 by Using Nanoelectrospray MS and MS/MS

TitleIdentification of Amino Acid Substitutions in Avian Influenza Virus (H5N1) Matrix Protein 1 by Using Nanoelectrospray MS and MS/MS
Authors
Issue Date2009
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jasms
Citation
Journal Of The American Society For Mass Spectrometry, 2009, v. 20 n. 2, p. 312-320 How to Cite?
AbstractMatrix protein 1 (M1), the major structural protein of the avian influenza virus, plays a critical role in regulation of viral RNA transcription via interaction with RNA and transportation of RNP cores. Mutations in M1 have been frequently observed in the highly virulent avian influenza H5N1 virus, which might be crucial to the pathogenic function. Here we report the characterization of mutated peptides in M1 purified from highly pathogenic avian influenza virus H5N1 by nanoelectrospray MS and MS/MS analyses on a quadrupole-time-of-flight mass spectrometer (Q-TOFMS). The specificity of tandem mass spectrometry allowed the identification of six amino acid (AA) substitutions in M1, including R95K, A166V, I168T, N207S, N224S, and R230K. Two commonly observed modifications such as oxidation and deamidation were accurately assigned in the protein. Bioinformatics analysis suggested some relationship between the amino acid substitution and structural property of M1 protein. Discussions on de novo sequencing of MS/MS spectra, especially in dealing with the AA substitutions, were provided. © 2009 American Society for Mass Spectrometry.
Persistent Identifierhttp://hdl.handle.net/10722/59420
ISSN
2015 Impact Factor: 3.031
2015 SCImago Journal Rankings: 1.322
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, Nen_HK
dc.contributor.authorSong, Wen_HK
dc.contributor.authorLee, KCen_HK
dc.contributor.authorWang, Pen_HK
dc.contributor.authorChen, Hen_HK
dc.contributor.authorCai, Zen_HK
dc.date.accessioned2010-05-31T03:49:44Z-
dc.date.available2010-05-31T03:49:44Z-
dc.date.issued2009en_HK
dc.identifier.citationJournal Of The American Society For Mass Spectrometry, 2009, v. 20 n. 2, p. 312-320en_HK
dc.identifier.issn1044-0305en_HK
dc.identifier.urihttp://hdl.handle.net/10722/59420-
dc.description.abstractMatrix protein 1 (M1), the major structural protein of the avian influenza virus, plays a critical role in regulation of viral RNA transcription via interaction with RNA and transportation of RNP cores. Mutations in M1 have been frequently observed in the highly virulent avian influenza H5N1 virus, which might be crucial to the pathogenic function. Here we report the characterization of mutated peptides in M1 purified from highly pathogenic avian influenza virus H5N1 by nanoelectrospray MS and MS/MS analyses on a quadrupole-time-of-flight mass spectrometer (Q-TOFMS). The specificity of tandem mass spectrometry allowed the identification of six amino acid (AA) substitutions in M1, including R95K, A166V, I168T, N207S, N224S, and R230K. Two commonly observed modifications such as oxidation and deamidation were accurately assigned in the protein. Bioinformatics analysis suggested some relationship between the amino acid substitution and structural property of M1 protein. Discussions on de novo sequencing of MS/MS spectra, especially in dealing with the AA substitutions, were provided. © 2009 American Society for Mass Spectrometry.en_HK
dc.languageengen_HK
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/jasmsen_HK
dc.relation.ispartofJournal of the American Society for Mass Spectrometryen_HK
dc.subject.meshAmino Acid Sequenceen_HK
dc.subject.meshAmino Acid Substitutionen_HK
dc.subject.meshAnimalsen_HK
dc.subject.meshChick Embryoen_HK
dc.subject.meshInfluenza A Virus, H5N1 Subtype - chemistryen_HK
dc.subject.meshMolecular Sequence Dataen_HK
dc.subject.meshNanotechnology - methodsen_HK
dc.subject.meshSpectrometry, Mass, Electrospray Ionization - methodsen_HK
dc.subject.meshTandem Mass Spectrometry - methodsen_HK
dc.subject.meshViral Matrix Proteins - chemistry - geneticsen_HK
dc.titleIdentification of Amino Acid Substitutions in Avian Influenza Virus (H5N1) Matrix Protein 1 by Using Nanoelectrospray MS and MS/MSen_HK
dc.typeArticleen_HK
dc.identifier.emailChen, H:hlchen@hkucc.hku.hken_HK
dc.identifier.authorityChen, H=rp00383en_HK
dc.description.naturelink_to_OA_fulltext-
dc.identifier.doi10.1016/j.jasms.2008.10.010en_HK
dc.identifier.pmid19019697-
dc.identifier.scopuseid_2-s2.0-58249089286en_HK
dc.identifier.hkuros163962en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-58249089286&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume20en_HK
dc.identifier.issue2en_HK
dc.identifier.spage312en_HK
dc.identifier.epage320en_HK
dc.identifier.isiWOS:000263004500017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, N=36079934800en_HK
dc.identifier.scopusauthoridSong, W=23490429800en_HK
dc.identifier.scopusauthoridLee, KC=14060567400en_HK
dc.identifier.scopusauthoridWang, P=13907209900en_HK
dc.identifier.scopusauthoridChen, H=26643315400en_HK
dc.identifier.scopusauthoridCai, Z=7402904946en_HK

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